Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,820,156	T→G	Q619P (CAG→CCG)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,820,156	0	T	G	100.0%	112.3 / NA	32	Q619P (CAG→CCG)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/16);  total (16/16)

CCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCGCAGC  >  NC_000913/2819913‑2820400                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ccGGTTACCGGTTGAATACGACGAACGCCTGCACCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTTCGGGTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCggtgt                                                                                                                                                                                                                                                  <  1:200815/248‑1 (MQ=255) ccGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGAGGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGtt                                                                                                                                                                                                                                               <  1:53702/251‑1 (MQ=255)  cGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGACGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTc                                                                                                                                                                                                                                              <  1:114372/251‑1 (MQ=255)       aCCGCGTCGATACGACGAACGCCTGCAGCAGTACCCAATTCAGAGATGATGCGAAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCa                                                                                                                                                                                                                                          <  2:221369/250‑1 (MQ=255)            ttCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCtt                                                                                                                                                                                                                                    >  2:7202/1‑251 (MQ=255)              cGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCg                                                                                                                                                                                                                                  >  1:108951/1‑251 (MQ=255)                                        cccGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCAt                                                                                                                                                                                                        <  1:89611/251‑1 (MQ=255)                                         ccGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTCCGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGTTCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGCCTTAGACCGCACGAATCTCTCCTGGTTTCATc                                                                                                                                                                                                       >  2:180231/1‑251 (MQ=255)                                               cAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCg                                                                                                                                                                                                 <  1:74155/251‑1 (MQ=255)                                                     tgatgCGAAACAGACCAATATCACCAGTGCGGCTGGCGCGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTtgtgt                                                                                                                                                                                           >  2:94562/1‑251 (MQ=255)                                                     ggatgCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCTCCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTtgtgt                                                                                                                                                                                           <  2:188572/250‑1 (MQ=255)                                                            gAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATTTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGt                                                                                                                                                                                    >  1:104167/1‑251 (MQ=255)                                                              aCAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCg                                                                                                                                                                                  >  2:21565/1‑251 (MQ=255)                                                                                ggCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCg                                                                                                                                                                <  2:217861/250‑1 (MQ=255)                                                                                   ggCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGtt                                                                                                                                                              >  1:61658/1‑250 (MQ=255)                                                                                           tGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTg                                                                                                                                                      >  1:122584/1‑250 (MQ=255)                                                                                                          aaCTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATAc                                                                                                                                      >  1:74329/1‑251 (MQ=255)                                                                                                                               aTGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGgcg                                                                                                                 <  1:94562/251‑1 (MQ=255)                                                                                                                                       cACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTtgtg                                                                                                                                                                                            >  1:154499/1‑168 (MQ=255)                                                                                                                                       cACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTtgtg                                                                                                                                                                                            <  2:154499/168‑1 (MQ=255)                                                                                                                                                    acatcatACTTCTCGCAGAAGAGCGCCAGCGCACCTTTCGCTTTCGCCGCTTCGAGCTCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGCACCTGGCGCAGCGCAGCGTACATCAGGTg                                                                                            >  2:183898/2‑251 (MQ=255)                                                                                                                                                     cagcctACTTCTCTCCGAACTACGTCCTCGCCCCTTTCGCTTTCGCCGCTTCGATAACTATGATGTTGTTTTTGATCGGCAAATTGCGACTAATTGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTGTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGGCGAAGCGCAGCGCCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATTAGTACCCAGAACCTGGCGCAGCGCATCGTGTATCAGGTGc                                                                                           <  2:222750/246‑1 (MQ=255)                                                                                                                                                           aCTTCTCGCCGAACAGAGCCATCGCACCTTTCGCTCTCGCCGCTTGGAGATCCATGATGTTGGGTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGGTGTGGGAGAAGTCGAAGGGCAGCGCCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCCGCGCAGCGTGCATCAGGTGCGTTggg                                                                                     <  2:60686/251‑3 (MQ=255)                                                                                                                                                                   ccGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGAtt                                                                             <  1:83736/251‑1 (MQ=255)                                                                                                                                                                         aGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCACATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGACGAAGCGCAGCACCTTGTAGTTAACCAGTGAACCTTTCGGCGATACCTGAGTACCCAGAACCTGGCGCAGCGCTGCGTGCATCAGAGGAGTTGCGGaacgatacagatg                                                                       >  2:68696/1‑239 (MQ=255)                                                                                                                                                                              cATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATAc                                                                  <  2:153412/251‑1 (MQ=255)                                                                                                                                                                                  gCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACAAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGgc                                                              >  1:144760/1‑251 (MQ=255)                                                                                                                                                                                   cACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGgcg                                                             <  1:190713/251‑1 (MQ=255)                                                                                                                                                                                           gctttcgaCGCTTCGAGATCAATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGc                                                     >  2:79073/1‑251 (MQ=255)                                                                                                                                                                                              ttcgcCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCt                                                   <  1:186959/250‑1 (MQ=255)                                                                                                                                                                                                          agaTCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGc                                      >  2:4084/1‑251 (MQ=255)                                                                                                                                                                                                                                           cgacgaATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCgc     <  2:9975/251‑1 (MQ=255)                                                                                                                                                                                                                                              cgaATCGGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGCGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGCACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATAAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCccagc  >  2:56776/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     CCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCAGTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACCAGTGAACCTTTCTGCGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCGCAGC  >  NC_000913/2819913‑2820400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑