Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,135,180	IS2 (–) +5 bp	coding (1346‑1350/2181 nt)	katG →	catalase/hydroperoxidase HPI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4135180 =	0 (0.000)	20 (0.920)	19/490	0.3	100%	coding (1346/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4135184	0 (0.000)	24 (1.100)	23/490	0.1	100%	coding (1350/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134940‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGC  <  NC_000913/4499511‑4499291                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    GTAAACCGACAATGCTGGTGACCGACCTGCCGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAGCGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATT                                                                                                                                                                                                                         <  2:60018/251‑1              GCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATT                                                                                                                                                                                                             <  1:194988/250‑1                 GGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTC                                                                                                                                                                                                           <  1:184874/249‑1                   TGACCGACCTTACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGGGCCGAAAGAAGATCTGATCTGGCTAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCC                                                                                                                                                                                                          >  2:169189/1‑248                     ACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCTTGCCTGGTTAAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAAACCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTACAGAC                                                                                                                                                                                                      >  1:205405/1‑250                       CGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATC                                                                                                                                                                                                   <  2:153587/251‑1                        aaccggacgcttctgttttATCCAGAGTTCGAGAAGGTCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCT                                                                                                                                                                                                  <  2:114697/232‑1                             GACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGAGCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTA                                                                                                                                                                                              <  1:145413/250‑1                                                               TCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGACTTTACCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGACCGCTGCCGCAGAT                                                                                                                                                            >  1:10917/1‑250                                                                   GTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCC                                                                                                                                                       <  2:23016/251‑1                                                                          CCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAACGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCG                                                                                                                                                >  2:138105/1‑251                                                                            cCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAG                                                                                                                                              <  1:148429/250‑1                                                                                                            GCCCGTGCATGGTTCAAACTGACGCAACGTGATATGGGGCCGAAATCTCGCTACATCGGGCCTGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCTATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTC                                                                                                              <  2:211480/251‑1                                                                                                                GTGCCTGGTTCCAACAGACGCACAGGGATCTGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGCAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGCTCTACAACCCGACAGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCCTTCGTTc                                                                                                          >  2:17259/1‑250                                                                                                                     TGGTTCAAACTGACGCACAGGGATATGGGGCCGACATCTCGCTACATCGAGCCGGCACACCCGCAAGCAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTCCAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATAACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGAGGATGCCATTCGTTATAATG                                                                                                     >  1:139862/1‑251                                                                                                                                             ATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCTACCGAGCAGGCCATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCAGTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCT                                                                              <  1:176902/250‑1                                                                                                                                                GGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGC                                                                          <  1:167003/251‑1                                                                                                                                                      AAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAATCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGCACGCCTCTGCAAGGTTCTTTGCTGCCGT                                                                    >  2:115608/1‑251                                                                                                                                                                               TGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGCTCTACAACCCGCCCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCCTTACAAGCCCGCTGCCGCAGATATTGCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAACGTTCGAACCCCTCTGCAAGGTTCTCTGCTGCCGTTAACCCGTCTGGTTTGGGCATGAT                                            >  1:156953/1‑250                                                                                                                                                                                   gacAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTG                                        >  2:17708/4‑250                                                                                                                                                                                                                 gcaGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTT‑ATCGTTTTCACGAAGCT        <  2:54442/248‑1                                                                                                                                                                                                                 GCCGCAGCCGATCTACAACCCGACCGATCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGC         <  1:89202/251‑1                                                                                                                                                                                                                       GCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTG   <  2:161164/251‑1                                                                                                                                                                                                                        CCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGC  >  1:208899/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    GTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134940‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGC  <  NC_000913/4499511‑4499291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.