Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,737,137	IS2 (+) +5 bp	coding (37‑41/825 nt)	bax ←	putative glycoside hydrolase Bax

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3187426	NA (NA)	16 (0.740)	15/488	0.4	100%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3737137 =	0 (0.000)					coding (41/825 nt)	bax	putative glycoside hydrolase Bax
*	?	NC_000913	= 3737141	0 (0.000)	27 (1.240)	26/490	0.1	100%	coding (37/825 nt)	bax	putative glycoside hydrolase Bax
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

AATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187202‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTT  >  NC_000913/3737137‑3737377                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    AATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTAT                                                                                                                                                                                                                         >  1:209109/1‑251      CAGATAGCTTCGTGAAAACGATAAAGCGTGACTGCATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACATTGCAGAGGCAATCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGTATATCGCTCGCAACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGATAAGTGATAACATATGGCTGGCAATATAGGGGCAATTCCTAGAATCATCGCCCCATCTCGTCGGATGGGcg                                                                                                                                                                                                                    >  2:191930/1‑249            gaTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAA                                                                                                                                                                                                              <  2:63695/249‑1                            AAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGG                                                                                                                                                                                              >  1:191473/1‑251                                         ATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAAGCATCAGGTCTCCTGGTCGGATTTAATCA                                                                                                                                                                                 <  1:189755/251‑1                                         ATCAGTATCATGCCCAAACCAGACGGGGTAACGGCAGCAAAGAACCTTGCAGAGACTTTAGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATATGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATC                                                                                                                                                                                  >  2:170974/1‑250                                                             AGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGTTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGATTGTAAGGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCTGCATATCGTCGTATGGGAGTCAAAATCAGCAGGTCTCCTGCGCTGATTTAATCATTCCAACACCTTATATTTTT                                                                                                                                                             <  1:209389/251‑1                                                                  GGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATGATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCTTATGGGAGTCAAAATCATCAGGTCTCCTGGTCTGATTTAATCATTCCAACACCTTATATTTTTCACAA                                                                                                                                                        <  2:109878/251‑1                                                                                                          TTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATAATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGT                                                                                                                >  1:86340/1‑251                                                                                                                                                                                          tcAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAA                                 >  2:86805/3‑250                                                                                                                                                                                                         GTCTGGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCG                  <  1:86805/250‑1                                                                                                                                                                                                             GGAAATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAA             <  1:82080/251‑1                                                                                                                                                                                                                 ATATAGGGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCA         <  1:122575/251‑1                                                                                                                                                                                                                   ATAGGGGAAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTACTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGC       <  1:195648/251‑1                                                                                                                                                                                                                       GGGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATCTCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACGATGTCACGTGTTAACGATTCAGATTGGCGCTAAATGGCAGAAAATGTGGGGGTTGACGAAAAATTCAGCCGTT   <  1:68588/251‑1                                                                                                                                                                                                                        GGCAAATCCAAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTT  <  1:23067/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    AATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187202‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aAGAATCATCGCCCCATATCGTCGTATGGGAGTCAAAATCATCAGGTCTCCTGGTCGGATTTAATCATTCCAACACCTTATATTTTTCACAAATTTGAGAGTTGAATCTCAAATCATATCAAAAATAGCTGTCAAGAGCACCCCAAGGAATAGTCCAAATCTGAAACTATGTCACGTGTTAACGATTCAGATTGGCGCTAAATCGCAGAAAATGTGGGGGTTATCGCAAAATTCAGCCGTTT  >  NC_000913/3737137‑3737377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.