Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,751	Δ1 bp	coding (1295/1374 nt)	mdtK →	multidrug efflux pump MdtK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,751	0	C	.	100.0%	87.0 / NA	20	coding (1295/1374 nt)	mdtK	multidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (9/11);  total (9/11)

GCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCT  >  NC_000913/1744512‑1744962                                                                                                                                                                                                                                                |                                                                                                                                                                                                                    gctggctgCGCATTTTATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTatgat                                                                                                                                                                                                         <  2:117297/251‑1 (MQ=255)                            ggcggTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑TGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACg                                                                                                                                                                              <  1:41472/251‑1 (MQ=255)                                  atatCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGcc                                                                                                                                                                        >  2:103471/1‑251 (MQ=255)                                    atCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCg                                                                                                                                                                       <  1:73181/250‑1 (MQ=255)                                                 cTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCt                                                                                                                                                         >  1:26974/1‑251 (MQ=255)                                                      gccAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTGACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAAc                                                                                                                                                    <  1:132868/250‑1 (MQ=255)                                                                             ttGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCTCTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACa                                                                                                                              <  1:69524/250‑1 (MQ=255)                                                                                           aGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCAc                                                                                                               <  1:103471/251‑1 (MQ=255)                                                                                               aCGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTCTGCGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACgggg                                                                                                           >  2:74521/1‑250 (MQ=255)                                                                                                   gTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTgcg                                                                                                       <  1:126256/251‑1 (MQ=255)                                                                                                       cATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCAtt                                                                                                                                                           <  1:175143/195‑1 (MQ=255)                                                                                                       cATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCAtt                                                                                                                                                           >  2:175143/1‑195 (MQ=255)                                                                                                        aTTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGTTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGt                                                                                                  <  2:223048/251‑1 (MQ=255)                                                                                                                         tACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTgg                                                                                 >  1:135115/1‑251 (MQ=255)                                                                                                                         tACGGCTTACTGGGTGCTGGGCTTGCAAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTg                                                                                  <  2:119355/250‑1 (MQ=255)                                                                                                                                               ttgccacggggatataTTCTGGCACTCGCCGATCTGGTCGTTAAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCACCGAGCATCCCGCTAATACAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCACGCTGGAGAGTTGCGCCAATCCCGTCtt                                                           >  2:23723/13‑251 (MQ=255)                                                                                                                                                                   ggCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTCTGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTc                                          >  1:154810/1‑248 (MQ=255)                                                                                                                                                                      aCTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCg                                    >  2:99028/1‑251 (MQ=255)                                                                                                                                                                                                     ccAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGtt      >  2:432/1‑250 (MQ=255)                                                                                                                                                                                                        gcagGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGG‑AGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTAtct  <  1:99028/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                    GCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTGGCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTTATCT  >  NC_000913/1744512‑1744962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑