Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,6610CG55.9% 5.1 / 55.1 35T75T (ACG→ACC) tfaQputative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base G (9/10);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTA  >  NC_000913/1634435‑1634887
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   
cagcaTCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                                                                                                                                             <  1:185983/251‑1 (MQ=31)
cagcaTCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTGTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATTGTCTTCAACg                                                                                                                                                                                                             <  2:167927/251‑1 (MQ=11)
        tGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGTGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGCCTTCAACGAGATGccc                                                                                                                                                                                                     >  2:159810/1‑250 (MQ=11)
                                   aCCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑g                                                                                                                                                                          <  2:160090/250‑1 (MQ=31)
                                       gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTTCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCa                                                                                                                                                                       <  2:38344/249‑1 (MQ=14)
                                       gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACg                                                                                                                                                                                                   <  1:183331/222‑1 (MQ=31)
                                       gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATACGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACg                                                                                                                                                                                                   >  2:183331/1‑222 (MQ=14)
                                           cagtcttttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGGGCCGTTCCACTTCTGATATTCCCCTCCCGGCGAGAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAag                                                                                                                                                                  <  2:65943/243‑1 (MQ=11)
                                           caggcttttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCACCACTGTTGAAAACA‑GCCACAAagc                                                                                                                                                                 >  1:122160/8‑251 (MQ=11)
                                           cAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGCTGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAA‑CACGCCACGAagc                                                                                                                                                                 >  2:276596/1‑251 (MQ=9)
                                                  ttttttGTTACTTCCGCCTCCCCGACCCGGAACAGTTTTTCTGCTTCCGTCTCCGTCACCCAGGCTTTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCGCGTCATAGACGGTTTTACCCCGATGGGCTTCAACGAGATGCCACGATGCCTCATCACTGTTTAAATCA‑GCCCCAAAGCCAGCCg                                                                                                                                                           <  2:112562/250‑1 (MQ=11)
                                                   tttttGCTTCTTCCGACTCCCGGATCCGGAACAGTTTTTCTGCATCCGTAACCTTCACCCAGGCTGTGCCGTTCCACTTCTGCTATTCCCCTCCCGGCGATAACCAGGCAAAATTTTCCGGCAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTCTTACCCCGATGGTCTTCAACGAGATGCCACCATGCCTCATCACTGTTGAAAACA‑CCCACACAGCCAGCNggc                                                                                                                                                         >  2:262200/1‑250 (MQ=11)
                                                                   cTCCCGGATCCGGAACAGTTTTTCTGCTCCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGACACGATGCCTCATCACTGTTGAACACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCa                                                                                                                                          >  2:132663/1‑250 (MQ=11)
                                                                     cccGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAat                                                                                                                                        >  2:53523/1‑250 (MQ=31)
                                                                        ggATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACACAGGCTGTGCCGTTACACTTATGAAACTACCCTTCCGGCGATAACCAGGTAACCTTTTCCGGTCACGGACCGAGATCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACAGTTTTAGCCCGATGATCTTCAACGAGATGACACAATGCCTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCAGGCGTTGCAATCTCg                                                                                                                                    >  1:154346/1‑251 (MQ=11)
                                                                               gAACAGTTTTTCTGCTTCCGTATACTTCACCCAGGCTGTGCAGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGATCAGAAATAAATAACGCGACGCAGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GACACGAAGCCAGCCGGAATATCAGGCGGTGCAATATCGGTACTGt                                                                                                                             >  2:275872/1‑251 (MQ=11)
                                                                                 aCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTAcccc                                                                                                                                                                                                                           <  1:259003/156‑1 (MQ=11)
                                                                                 aCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTTTTAcccc                                                                                                                                                                                                                           >  2:259003/1‑156 (MQ=11)
                                                                                    gTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCCCTGTTTAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                                                                         <  1:167460/250‑1 (MQ=11)
                                                                                      ttttCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATAGGTACTTTTTGcagg                                                                                                                      >  1:35696/1‑248 (MQ=11)
                                                                                               tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTg                                                                                                              <  2:49556/250‑1 (MQ=31)
                                                                                                         tCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCgg                                                                                                    <  2:201881/250‑1 (MQ=31)
                                                                                                          cACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCTCGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGaa                                                                                                  <  1:152209/251‑1 (MQ=11)
                                                                                                                 gCTGTGCCGTTCCACTTCCGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCGGTTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCAGAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGAGGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGTCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGc                                                                                           <  1:269444/251‑1 (MQ=11)
                                                                                                                    gtgCCGTTCCACTTCTGAAACTCCCCTTCCGTCGATAACCAGGTAACATTTTCCGTTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGTAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCa                                                                                          <  2:196572/249‑1 (MQ=14)
                                                                                                                           tCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTc                                                                                  >  1:110437/1‑250 (MQ=11)
                                                                                                                                ttCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGAACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAAt                                                                            >  2:181379/1‑251 (MQ=14)
                                                                                                                                   tGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATaa                                                                          >  2:77342/1‑250 (MQ=31)
                                                                                                                                       attCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTc                                                                      >  1:177559/3‑250 (MQ=11)
                                                                                                                                                             ggTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATtat                                               <  2:195014/251‑1 (MQ=11)
                                                                                                                                                                   aTTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAAttt                                         >  1:11385/1‑251 (MQ=11)
                                                                                                                                                                     tttCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAAttttt                                       >  2:103370/1‑251 (MQ=31)
                                                                                                                                                                         cGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTCCCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATgg                                   <  2:6980/251‑1 (MQ=11)
                                                                                                                                                                                         tCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCAc                    <  2:35696/250‑1 (MQ=11)
                                                                                                                                                                                                          gTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTa  <  2:74590/251‑1 (MQ=31)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                   
CAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTATAAATTTTTATGGTCCGTGGTTGTTCACTCATTCTGAATGCCATTA  >  NC_000913/1634435‑1634887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑