Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,634,5970CA57.1% 0.7 / 46.5 35V97F (GTT→TTT) tfaQputative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base A (9/11);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TCCAGCAACGAGATTTCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTAT  >  NC_000913/1634392‑1634842
                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
tCCAGCAACGAGGTTTCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGcc                                                                                                                                                                                                           >  1:195014/1‑251 (MQ=11)
  cAGCAACGAGATTTCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCgg                                                                                                                                                                                                         <  1:172300/251‑1 (MQ=31)
        cGAGGTTTCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTCCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCa                                                                                                                                                                                                   <  1:165465/251‑1 (MQ=11)
         gaggtttCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCAc                                                                                                                                                                                                  <  1:18090/247‑1 (MQ=11)
                      ccGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAAGTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATagacggt                                                                                                                                                                                      <  2:163174/250‑7 (MQ=11)
                                           cagcaTCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                                                                                                <  1:185983/251‑1 (MQ=31)
                                           cagcaTCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTGTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATTGTCTTCAACg                                                                                                                                                                <  2:167927/251‑1 (MQ=11)
                                                   tGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGCTTTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGTGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGCCTTCAACGAGATGccc                                                                                                                                                        >  2:159810/1‑250 (MQ=11)
                                                                              aCCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑g                                                                                                                             <  2:160090/250‑1 (MQ=31)
                                                                                  gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTTCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCa                                                                                                                          <  2:38344/249‑1 (MQ=14)
                                                                                  gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACg                                                                                                                                                      <  1:183331/222‑1 (MQ=31)
                                                                                  gCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATACGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACg                                                                                                                                                      >  2:183331/1‑222 (MQ=14)
                                                                                      cagtcttttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGGGCCGTTCCACTTCTGATATTCCCCTCCCGGCGAGAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAag                                                                                                                     <  2:65943/243‑1 (MQ=11)
                                                                                      caggcttttttttGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCACCACTGTTGAAAACA‑GCCACAAagc                                                                                                                    >  1:122160/8‑251 (MQ=11)
                                                                                      cAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGCTGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAA‑CACGCCACGAagc                                                                                                                    >  2:276596/1‑251 (MQ=9)
                                                                                             ttttttGTTACTTCCGCCTCCCCGACCCGGAACAGTTTTTCTGCTTCCGTCTCCGTCACCCAGGCTTTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCGCGTCATAGACGGTTTTACCCCGATGGGCTTCAACGAGATGCCACGATGCCTCATCACTGTTTAAATCA‑GCCCCAAAGCCAGCCg                                                                                                              <  2:112562/250‑1 (MQ=11)
                                                                                              tttttGCTTCTTCCGACTCCCGGATCCGGAACAGTTTTTCTGCATCCGTAACCTTCACCCAGGCTGTGCCGTTCCACTTCTGCTATTCCCCTCCCGGCGATAACCAGGCAAAATTTTCCGGCAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTCTTACCCCGATGGTCTTCAACGAGATGCCACCATGCCTCATCACTGTTGAAAACA‑CCCACACAGCCAGCNggc                                                                                                            >  2:262200/1‑250 (MQ=11)
                                                                                                              cTCCCGGATCCGGAACAGTTTTTCTGCTCCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGACACGATGCCTCATCACTGTTGAACACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCa                                                                                             >  2:132663/1‑250 (MQ=11)
                                                                                                                cccGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAat                                                                                           >  2:53523/1‑250 (MQ=31)
                                                                                                                   ggATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACACAGGCTGTGCCGTTACACTTATGAAACTACCCTTCCGGCGATAACCAGGTAACCTTTTCCGGTCACGGACCGAGATCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACAGTTTTAGCCCGATGATCTTCAACGAGATGACACAATGCCTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCAGGCGTTGCAATCTCg                                                                                       >  1:154346/1‑251 (MQ=11)
                                                                                                                          gAACAGTTTTTCTGCTTCCGTATACTTCACCCAGGCTGTGCAGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGATCAGAAATAAATAACGCGACGCAGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GACACGAAGCCAGCCGGAATATCAGGCGGTGCAATATCGGTACTGt                                                                                >  2:275872/1‑251 (MQ=11)
                                                                                                                            aCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTAcccc                                                                                                                                                                              <  1:259003/156‑1 (MQ=11)
                                                                                                                            aCAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCAACGTCATAGACGGTTTTAcccc                                                                                                                                                                              >  2:259003/1‑156 (MQ=11)
                                                                                                                               gTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCCCTGTTTAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc                                                                            <  1:167460/250‑1 (MQ=11)
                                                                                                                                 ttttCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATAGGTACTTTTTGcagg                                                                         >  1:35696/1‑248 (MQ=11)
                                                                                                                                          tCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTg                                                                 <  2:49556/250‑1 (MQ=31)
                                                                                                                                                    tCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCgg                                                       <  2:201881/250‑1 (MQ=31)
                                                                                                                                                     cACCCAGGCTGTGCCGTTCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCTCGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGaa                                                     <  1:152209/251‑1 (MQ=11)
                                                                                                                                                            gCTGTGCCGTTCCACTTCCGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCGGTTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCAGAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGAGGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGTCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGc                                              <  1:269444/251‑1 (MQ=11)
                                                                                                                                                               gtgCCGTTCCACTTCTGAAACTCCCCTTCCGTCGATAACCAGGTAACATTTTCCGTTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGTAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCa                                             <  2:196572/249‑1 (MQ=14)
                                                                                                                                                                      tCCACTTCTGATATTCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTc                                     >  1:110437/1‑250 (MQ=11)
                                                                                                                                                                           ttCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGAACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAAt                               >  2:181379/1‑251 (MQ=14)
                                                                                                                                                                              tGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATaa                             >  2:77342/1‑250 (MQ=31)
                                                                                                                                                                                  attCCCCTCCCGGCGATAACCAGGTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTc                         >  1:177559/3‑250 (MQ=11)
                                                                                                                                                                                                        ggTAAAATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAGACGGTTTTACCCCGATGGTCTTCAACGAGATGCCACGATGCCTCATCACTGTTGAAAACA‑GCCACAAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCAGGCAGACCGGTATGAGGCGGAATATATGCGTCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATtat  <  2:195014/251‑1 (MQ=11)
                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
TCCAGCAACGAGATTTCTTCCTCCGTTGCAATTTCCAGATCTGCAGCATCCTGAAGCGGCGCAATATGCTCACTGGCTACCTGCATCAGGTTGTTTTTTGTTTCTTCCGCCTCCCGGATCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACA‑GCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTGGCAGACCTGTATGAGGCGGAATATATGCATCACCTTCACCAATAAATTCATTAGTTCCGGCCAGCAGATTAT  >  NC_000913/1634392‑1634842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑