Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,128	0	A	G	55.9%	29.3 / 66.3	59	K27R (AAA→AGA)	stfR	putative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base A (17/9);  new base G (17/16);  total (34/25)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGG  >  NC_000913/1428896‑1429376                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         ccgtcgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACATAACTGCTCCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACgg                                                                                                                                                                                                                                         <  2:138272/241‑1 (MQ=255)                  gCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTgg                                                                                                                                                                                                                       <  2:105552/251‑1 (MQ=255)                            gctccGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGagaa                                                                                                                                                                                                             <  1:236472/248‑4 (MQ=255)                               ccGGCAGTGGCGGCTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATc                                                                                                                                                                                                           <  2:94541/250‑1 (MQ=255)                               ccGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATc                                                                                                                                                                                                           <  2:219515/250‑1 (MQ=255)                                         cGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCAAGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGcc                                                                                                                                                                                                >  1:62573/1‑251 (MQ=255)                                              ggCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGGGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCAACACGGTGGTGGTGAACAAGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCg                                                                                                                                                                                           >  2:4148/1‑251 (MQ=255)                                                     tGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGAACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTCCAGc                                                                                                                                                                                    >  1:221935/1‑251 (MQ=255)                                                      gACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAg                                                                                                                                                                                     <  1:151649/249‑1 (MQ=255)                                                        cggattCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATg                                                                                                                                                                                 <  1:265776/247‑1 (MQ=255)                                                        cGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATg                                                                                                                                                                                 >  1:19160/1‑251 (MQ=255)                                                          gTTTCATCGTCGGTATCGGTTATCAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGATTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGGGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GAAAAACGTAACAGGACCACCTTGGTGGAGAACACGCTGGCCTAAGAAAATCCGGATACAGCCGGTCGTGACAGCATgtg                                                                                                                                                                               >  2:27811/1‑249 (MQ=255)                                                           actCATCGTTGGGGCCGGTTATAAATTCTGATTAGACAGGTACCACAGTGTTATGACAGCCCGCCGGAACCGGTGGGTTTTTTCGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTGCTGAAAGACGGCACAGGGAAACCGGGAAAGAAATGCACCATTAAGCTGAAA‑GCCAGACGGAAAAGCACCACAGTGGAGGTGAAGAAGGTGGGCTAAGAGAATCAGAATGAAGACGGGCGATAAAGCaanga                                                                                                                                                                               >  2:252723/3‑246 (MQ=255)                                                                 cgttggggtCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGatgtggag                                                                                                                                                                        <  2:230321/244‑8 (MQ=255)                                                                    tggggtCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTa                                                                                                                                                                      <  1:64067/246‑1 (MQ=255)                                                                      gggtCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACgg                                                                                                                                                                   <  2:95662/249‑1 (MQ=255)                                                                         tCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAg                                                                                                                                                                                                                                                 >  2:247889/1‑170 (MQ=255)                                                                         tCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAg                                                                                                                                                                                                                                                 <  1:247889/170‑1 (MQ=255)                                                                              tataAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTAc                                                                                                                                                            >  1:178478/1‑250 (MQ=255)                                                                               ataAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCTGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGGAACCGCACCACGGTGGTGGTGAACACGCTGACCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTAc                                                                                                                                                            >  1:110224/1‑249 (MQ=255)                                                                                taAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGc                                                                                                                                                         >  2:30462/1‑251 (MQ=255)                                                                                      cTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTAtt                                                                                                                                                   >  1:61033/1‑251 (MQ=255)                                                                                           tAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTTCAGCGTTATTCTGt                                                                                                                                               <  1:192861/250‑1 (MQ=255)                                                                                            aGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAgtgtcatcctnc                                                                                                                                               >  2:259729/1‑238 (MQ=255)                                                                                            aGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGGGGAGTACGGTCAGTACAgtgtcatcctgc                                                                                                                                               >  2:141663/1‑238 (MQ=255)                                                                                               cAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggtt                                                                                                                                          >  1:45618/1‑235 (MQ=255)                                                                                                  gTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGt                                                                                                                                                              >  1:83077/1‑228 (MQ=255)                                                                                                  gTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGt                                                                                                                                                              <  2:83077/228‑1 (MQ=255)                                                                                                      cacaGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACggtggtggt                                                                                                                                                                                                                                  <  1:242048/156‑1 (MQ=255)                                                                                                      cacaGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACggcggtggt                                                                                                                                                                                                                                  >  2:242048/1‑156 (MQ=255)                                                                                                         aGTGTTATGACAGCACGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAAAGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTcc                                                                                                                                >  2:36495/1‑251 (MQ=255)                                                                                                                          ccgGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GAAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTGGGTGGAAGGATTCCCGCCGTCACATGCCggg                                                                                                               <  1:229607/251‑1 (MQ=255)                                                                                                                                      ggCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCgtg                                                                                                   >  2:206035/1‑251 (MQ=255)                                                                                                                                                    gTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcaca                                                                                     <  2:62573/251‑4 (MQ=255)                                                                                                                                                     tGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACCGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcaca                                                                                     >  2:225220/1‑247 (MQ=255)                                                                                                                                                      gAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGGAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAAAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAAc                                                                                   >  2:99797/1‑251 (MQ=255)                                                                                                                                                            ggaaGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGTACTGCACCATTCAGCTGAAATGCAGA‑CGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTAAGAGAATCCGGATGAAGCCGGGCGTGACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGTCGGGGCCATCACCGTGTATGAAGATTCCCAAccgggga                                                                             <  2:227830/248‑6 (MQ=255)                                                                                                                                                              aaGTAAAGATTTCAGGTGTAAGGAAAGACGGCCAAGGAAAACGGTTACAGAACGGCACAATCCAGCTGAAA‑GCAAACGGTAAGAGCACCACGTTGGTGGTGAACGCGCTGGCCTCGGAAAATCCGGATGAAGCCGGGCGTTACAGCTTGGACGTTGAGTACGGTCAGTCCAGCGTTAGTCTGTTGGTGGAAGTGTTGCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACg                                                                           <  1:109299/250‑1 (MQ=255)                                                                                                                                                                gTAAAGATTTCAGGTGTACTGAAAGACGGCACAGAAAACCCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAACCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCt                                                                         <  1:55765/251‑1 (MQ=255)                                                                                                                                                                  aaaGATTTCACGTGTACTGCAAGACGGCACAGGAAAACCGGAACAGCTCTCCAAACTCAAGCTGAAA‑GCAAAACGTAACAGCACCACTGAGGTGGTGCACCCACTGGCCTCAGAACATCCTGATGAAGCCGGGCGCTCCAGCCTGGACGTTGCGTACGGTCCGTACCGCGTTCTTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGATGATTCTCAACCCGGTACGCTgc                                                                       >  2:42664/1‑250 (MQ=255)                                                                                                                                                                    aGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAAt                                                                     <  1:141663/251‑1 (MQ=255)                                                                                                                                                                         tcagtagtccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACCGCTCCACGGTGGTGGTGAACACGGTGGGCTCAGAGACTCCGGATGAAGCCGGGCTTTACa                                                                                                                                                                                      <  2:129091/124‑1 (MQ=255)                                                                                                                                                                         tCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACa                                                                                                                                                                                      >  1:129091/1‑133 (MQ=255)                                                                                                                                                                              tgtACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCg                                                           >  1:41676/1‑251 (MQ=255)                                                                                                                                                                                    gAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGcc                                                      >  2:214565/1‑250 (MQ=255)                                                                                                                                                                                      aaGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATg                                                   >  2:136597/1‑251 (MQ=255)                                                                                                                                                                                        gACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGAc                                                 >  2:77606/1‑251 (MQ=255)                                                                                                                                                                                           ggCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACgg                                               >  1:124276/1‑250 (MQ=255)                                                                                                                                                                                                 ggAAAACCGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGgatga                                        <  1:90499/251‑1 (MQ=255)                                                                                                                                                                                                      aCCGGTACAAAACTTCATAATCCAGCTGAAC‑GCAAAACGTAACAGCACCACGGTGGTGTGGAACCCCCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTTTGGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGGTTATGAAGATTCTCAACCCGGTACGCTGAATGATTTGCTCGGTGCCATGACGGAGGATGATGccc                                   <  1:248010/251‑1 (MQ=255)                                                                                                                                                                                                        cGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCGCCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCgg                                 <  2:49299/251‑2 (MQ=255)                                                                                                                                                                                                        cGGTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCgg                                 >  1:92518/1‑250 (MQ=255)                                                                                                                                                                                                         ggTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggc                                >  2:27293/1‑249 (MQ=255)                                                                                                                                                                                                          gTACAGAACTGCACCATTCAGCTGAAA‑GCCAGACGCAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggcc                               >  2:199717/1‑248 (MQ=255)                                                                                                                                                                                                                aaCTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGc                         >  1:216701/1‑251 (MQ=255)                                                                                                                                                                                                                       caATCTAGATGAAA‑GCAAAACGTAACAGCACCACGGGGGAGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCAGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGAGTCCCGTCCGGAGGCACTgcgc                  >  1:231342/1‑251 (MQ=255)                                                                                                                                                                                                                             aGCTGAAA‑GCAAAACGTAACAGAACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGTTGCCATACCGGAGGATGATGCCCGTCCGGAGGCACTGCGCAGtttt            >  2:206302/1‑251 (MQ=255)                                                                                                                                                                                                                              gCTGAAA‑GCATAACGTACCGGCACCACGGTGGGGGTAAACGCCCTGGCCTAAGAAAAGCCGGTGGAAGCCGGGGGTTACTGATTGGCCTTTGAGTACGGTCATTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTg           <  2:94410/251‑1 (MQ=255)                                                                                                                                                                                                                               cTGAAA‑GCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCAGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcgtcttg           >  1:41709/1‑235 (MQ=255)                                                                                                                                                                                                                                        aaaaCGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGAtgg  >  1:132927/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                         CCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAA‑GCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGG  >  NC_000913/1428896‑1429376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑