Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,251,2720AC54.5% 4.2 / 12.0 11A69A (GCA→GCC) dhaRDNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (0/6);  total (3/8)
Fisher's exact test for biased strand distribution p-value = 6.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATCAACATTTCAAACAGGTACTCTGGAACTGGGCC  >  NC_000913/1251023‑1251497
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                 
aaaTTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCAc                                                                                                                                                                                                                                  >  1:155935/1‑251 (MQ=255)
                 cccTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGTCATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTg                                                                                                                                                                                                                 <  1:238606/250‑1 (MQ=255)
                           aTAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCg                                                                                                                                                                                                       <  1:141367/251‑1 (MQ=255)
                                           atGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCAt                                                                                                                                                                                       >  1:3573/1‑251 (MQ=255)
                                                                          aaagatctcctttattggaaaCCTACTGGGAGCAAGGCAATAAGCTGTTGAAACGGGAAAAATGGAACTAACAACATCAGAGCCAGGGTGTGTCATTGGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGCCGCGCGGGCAGGCGGCGCTGGAAGATGCCGGGAAAT‑ATATGGCCCGGCGAGAGGGTGTGTTGTTGATCCTCAATAAATCCGACTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTg                                                                                                                                                        <  2:222138/232‑1 (MQ=255)
                                                                                   ccTTAATTGCACCCTCCTGGGAGCGATGAAATAAGCTGATGAAACGGGAGACAGGGACCTTACCACATCAGGCCCAGGGCGTGACATTGGCTTCTATTTACCGGCGTAAGAAAGCGATGGTGACGCTCGGGCGGGCGGCGTTGGAATATGCCGGGGAAT‑ATATGGCCCCGCGAGAGTGTGCGTTGTTTATCCTCGATAAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTa                                                                                                                                               <  1:241482/251‑1 (MQ=255)
                                                                                                     gggAGCGATGCAATAAGCTGAGGAAACGGGAGACATGGAACGTACCACATCAGCCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGTTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGAAAT‑ATATGGCCCCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAAt                                                                                                                             <  2:221546/251‑1 (MQ=255)
                                                                                                                   aaGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTg                                                                                                               >  2:101682/1‑251 (MQ=255)
                                                                                                                                                                                                        tGCTGACGCGCGGGCAGGCTGCGCTGGAAGATCCCTGGGAAT‑ATTTGGCCCGGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATCAACAttt                           <  1:33878/250‑1 (MQ=255)
                                                                                                                                                                                                                   gggCAGGTTGCGCTGAAAGA‑GCCCGGGAATTATTGGGCCCCGGGAGAGGGTGCGTTTTTTATCCTCAAGAAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATCAACATTTCAAACAGGTAct               <  2:155935/251‑1 (MQ=255)
                                                                                                                                                                                                                                 ggAAGATCCCTGGGAAT‑TTAGGGCCCCGCGAGAGTTTGCGATGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATCAACATTTCAAACAGGTACTCTGGAACTGGGcc  <  2:208251/250‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                 
AAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTTAATTGCAACCTCCTGGGAGCGATGCAATAAGCTGATGAAACGGGAGACATGGAACGTACCACATCAGGCCCAGGGCGTGACATTTGCTTCTATTTATCGGCGTAAGAAAGCGATGCTGACGCTCGGGCAGGCTGCGCTGGAAGATGCCTGGGAAT‑ATATGGCACCGCGAGAGTGTGCGCTGTTTATCCTCGATGAAACCGCCTGCATTCTCAGCCGTAATGGCGATCCGCAAACCTTGCAGCAGCTAAGTGCACTGGGATTCAATGACGGCACGTATTGCGCCGAGGGAATTATTGGTACTTGTGCGCTATCGTTAGCGGCTATCTCTGGTCAGGCCGTGAAAACGATGGCCGATCAACATTTCAAACAGGTACTCTGGAACTGGGCC  >  NC_000913/1251023‑1251497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑