Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,536,236	IS5 (+) +4 bp	intergenic (‑2183/‑358)	topAI ← / → ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	20 (0.730)	20/488	0.4	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	4536236 =	0 (0.000)					intergenic (‑2183/‑361)	topAI/ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
*	?	NC_000913	= 2290113	NA (NA)	13 (0.470)	11/490	1.1	92.8%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 4536239	1 (0.040)					intergenic (‑2186/‑358)	topAI/ythB	toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

GATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/2288681‑2289161                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       aaTAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTGTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAAt                                                                                                                                                                                                                                            <  2:100920/250‑1 (MQ=255)              tAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAAACAGCCGGATTTTTTTTTCTATGAAAACACCCTCCACTGTCTCACCATACTCAAAAGTTTATGCCAAAGCTATTGGGGGATAAAAGGAAGGGGCGGATAAACGGGGCAAtt                                                                                                                                                                                                                               >  2:229028/1‑251 (MQ=255)              tAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                                                                                                                                <  1:207979/250‑1 (MQ=255)                      aGTATTCTGCGGCGCCATTCATTTCAATGTACGGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAGCTGTCTCGCCATACTGAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAGTTCTTCTCgg                                                                                                                                                                                                                       <  2:248753/251‑1 (MQ=255)                              tcggcgCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTTCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACt                                                                                                                                                                                                                <  2:2684/248‑1 (MQ=255)                                                   tACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCAAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTTCAGGACAACACCATCAACTGTCTCACCATACTCAACAGATGATTCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGGAATTCTTCTTGGCT‑GACTCAGTCCTTTCATTTGTTCAT‑Gtt                                                                                                                                                                                        >  1:177375/1‑251 (MQ=255)                                                           cccTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCCTTTCATTTCTTCAT‑GTTTGAGCCgg                                                                                                                                                                                >  1:147424/1‑250 (MQ=255)                                                                             attattACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCGGGTTATCTTTTTTTCCGACAACTCCTTCCACTCTCTCACCCTTCCCCACAGTTGATGCCCATGCTATGGGGGGAAGTTAGTGAGGAGCGGATAAAAGGGGAAATTCTTTTCGGCTAGA‑TCAGTCATTTCATTTCTTCATTGTTTG‑GGCGATTTTTTCTCCCGTacatt                                                                                                                                                              >  1:148559/1‑247 (MQ=255)                                                                               tattaCTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGc                                                                                                                                                             <  1:184746/250‑1 (MQ=255)                                                                                    cttacACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCTTCTTATTGGCGGTCCCCTAAACCAGCCGGTTATATTTTTTCAAGGCAACAAACTACACTGTTTCACCATACTCAACAGTTGATGCCCATGCGATGGGTGAAAATAAAGAAAGAGCGAATAAGCGGGGGAGTTCTTCTCGGCA‑GACTCCGTCATTTTCTTTCTTTTT‑GTTTTTGCCGCTTTTTTCTCCCGCAAATGCCTTGa                                                                                                                                                        >  2:170042/1‑250 (MQ=255)                                                                                     ttacACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCCGCTGGATATCTTTTTTCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTTATGCCAATGCTATTGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTTTCGGCT‑GAGTCAGACATTTTATTTCTTCAT‑GCTTGAGCCGATTTTTTCTCCCGAAAATTCCTTgcat                                                                                                                                                      >  1:48859/1‑248 (MQ=37)                                                                                              aTTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTTCCATAAACCAGCTGGATATCTTTTTTCAGGACCACACCATCAACTGTCTCACCCTACTCAACAGGTTATGCCAATGCTATGGGTGAATATAAGGGAGGGGCGAATAAGCGGGGAAATTCTTCTTGGCT‑GACTCAGTCCATTCCTTTTTTCAT‑GGTTTAGGCGTGTTTTTTTCCCGTAAATGCCTTGAAT‑CCGCCTAtt                                                                                                                                            >  1:24983/1‑251 (MQ=255)                                                                                                  ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGGTATCTTTTTTCCGGACAACACCATCAACTGTCTCACCATACTCAACAGTTTATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GGCTCAGTCATTTCATTTTTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTAttt                                                                                                                                           >  1:205828/1‑248 (MQ=255)                                                                                                               tctcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTTAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGGGCGAATAAGCGGGGGAATTCTTCTCGGCT‑GACTCCGTCATTTCATTTCTTCAT‑GTTTGCGCCGATTTTTTCTCCCGTAAATGCCTTTAATACA‑CCTATTTTAAACCGTT‑CTTCGcc                                                                                                                          >  2:66651/1‑251 (MQ=255)                                                                                                                           ttgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATATTTTTCCAGGACAACACCATCAAATGTCTCACCATACTCAACAGTTGATGCCCATGCTATGGGTGAATATAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTTAGCCGATTTTTTTTCCCGTAAATGCCTTGAAT‑TAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGGGtt                                                                                                              >  2:55915/1‑251 (MQ=25)                                                                                                                                              cGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTgag                                                                                           <  1:55915/251‑1 (MQ=255)                                                                                                                                                 tCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                             >  2:195718/1‑246 (MQ=255)                                                                                                                                                 tCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGAGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTg                                                                                             >  1:106388/1‑246 (MQ=255)                                                                                                                                                    cATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATcagatcggaagagcacacgttct                                                                                     >  1:25362/1‑229 (MQ=255)                                                                                                                                                    cATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATc                                                                                                           <  2:25362/229‑1 (MQ=255)                                                                                                                                                             gCTGGATATCTTTTTCCAGGACAACACCATCAACAGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGCATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTAAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGAc                                                                            >  1:261483/1‑251 (MQ=255)                                                                                                                                                                    atCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCa                                                                      <  1:134196/250‑1 (MQ=255)                                                                                                                                                                                         aTCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATTCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGtt                                                >  2:163612/1‑251 (MQ=255)                                                                                                                                                                                            aaCTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGc                                             >  2:83309/1‑251 (MQ=255)                                                                                                                                                                                                                          cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATc               <  1:15194/251‑1 (MQ=38)                                                                                                                                                                                                                             tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATcaa             >  1:236403/1‑248 (MQ=37)                                                                                                                                                                                                                                       gAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTCTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAc  <  2:205828/251‑1 (MQ=14)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       GATAACCGTCATTTAATACTGAGTATTCTGCGGCGCCATTCATTTCAATGTACTGATACCCTCCGTTAATTTCAGTATTATTACTTACACCAAATTCTTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCT‑GACTCAGTCATTTCATTTCTTCAT‑GTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAAT‑CAGCCTATTT‑AGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/2288681‑2289161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.