Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,255	C→T	V183V (GTG→GTA)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,255	0	C	T	100.0%	138.2 / NA	40	V183V (GTG→GTA)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

GATCCCACGGCAGCACCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCA  >  NC_000913/1260017‑1260499                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     gATCCCACGGCAGCACCAGTTGCGGCAGCAGTTGCGGAATACCGTTTCCCTGAGAACCATCCGCCAGGACGTAGTGGAATTGCGAACCGACGGTAGCAACATGTCCGCCGAGCAGGTTACCAATCCCCATCACCGCGCAACCATCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATCCCTAGCTTCACAATGCCAATGGCAGCATCACCTACATTAATGGTc                                                                                                                                                                                                                                          <  1:59970/251‑1 (MQ=255)           cagcaCCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCa                                                                                                                                                                                                                                <  2:289286/251‑1 (MQ=255)                   gTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTGGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAaataat                                                                                                                                                                                                                        >  2:182633/1‑251 (MQ=255)                      gCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATgc                                                                                                                                                                                                                      <  1:134338/250‑1 (MQ=255)                       cGGCAGAAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCCTCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCAAACGGCAGCATCACCTAACTTAATGGTCGGCAGCGCCATAAATACTgcgc                                                                                                                                                                                                                    >  2:335628/1‑251 (MQ=255)                                  gCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACACGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAACTAATGCGCCGACTTTTTg                                                                                                                                                                                                          >  1:209140/1‑250 (MQ=255)                                      aaTACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGt                                                                                                                                                                                                         >  1:277840/1‑247 (MQ=255)                                         aCCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAAt                                                                                                                                                                                                  <  1:204175/251‑1 (MQ=255)                                               aCCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCg                                                                                                                                                                                            <  2:287740/251‑1 (MQ=255)                                                 ccTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCggg                                                                                                                                                                                          >  2:81800/1‑251 (MQ=255)                                                   ggagaACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTGTGTAGATAATGTTCCGGGAc                                                                                                                                                                                        <  2:16360/250‑1 (MQ=255)                                                        aCCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCTCCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGTACATggg                                                                                                                                                                                   <  2:226564/251‑1 (MQ=255)                                                             cGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCAtt                                                                                                                                                                              <  2:198916/251‑1 (MQ=255)                                                               gCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTg                                                                                                                                                                            <  1:272954/251‑1 (MQ=255)                                                                                            ggTAGCATCATGTCCGCCGAGCAGGTTTACAATCCCCATCCCCGCTCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATTCCCAGACTCTGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCCCCTACATTAATGGTCGGCAGCGCCATAAATAATGCTCCGTCTTTTTTTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGATAAAATCTTTAATCTGCATg                                                                                                                                                <  1:178774/250‑1 (MQ=255)                                                                                                     aTGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATgg                                                                                                                                      <  1:274225/251‑1 (MQ=255)                                                                                                             cgAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCg                                                                                                                              <  2:224602/251‑1 (MQ=255)                                                                                                              gAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCg                                                                                                                              >  2:255938/1‑250 (MQ=255)                                                                                                                   ggTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATAccc                                                                                                                        <  2:144902/251‑1 (MQ=255)                                                                                                                     gtAACAATCCCCTTCCCCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTTCCGATGGTGATCCCGATACCCGa                                                                                                                      <  2:76780/250‑1 (MQ=255)                                                                                                                      tAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGGGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCg                                                                                                                       >  1:195339/1‑249 (MQ=255)                                                                                                                                         gcAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACgg                                                                                                  >  1:293270/1‑251 (MQ=255)                                                                                                                                                    gcAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAat                                                                                        >  2:172549/1‑250 (MQ=255)                                                                                                                                                      aaGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAatat                                                                                      >  2:128045/1‑250 (MQ=255)                                                                                                                                                        ggCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACt                                                                                   >  1:172681/1‑251 (MQ=255)                                                                                                                                                            ggAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAAt                                                                               <  1:332772/251‑1 (MQ=255)                                                                                                                                                               aGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATc                                                                              >  1:111733/1‑249 (MQ=255)                                                                                                                                                                 gTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGc                                                                          <  2:103529/251‑1 (MQ=255)                                                                                                                                                                  tGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCg                                                                         >  2:164050/1‑251 (MQ=255)                                                                                                                                                                          tAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGc                                                                 >  1:33696/1‑251 (MQ=255)                                                                                                                                                                                            cgGCCAAAACACAAGAATACCTAGCGTCACAATGCCACTGGCAGCATCACCTACATTACTGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATCCTGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGAGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGa                                                >  1:344504/1‑250 (MQ=255)                                                                                                                                                                                                cAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAAGCAGGCGACCAAAGCGGGCCAGACCCATCAGAATCaa                                           <  2:234818/251‑1 (MQ=255)                                                                                                                                                                                                  aaaaaaaaGCATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCaaa                                          >  2:216924/1‑250 (MQ=255)                                                                                                                                                                                                         aGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATccc                                  <  1:255938/251‑1 (MQ=255)                                                                                                                                                                                                           aaTACCTAGCGTCACAATGCCAATGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCg                                >  1:190839/1‑251 (MQ=255)                                                                                                                                                                                                                                aaTGGCAGCATCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACcagc            >  1:236610/1‑250 (MQ=255)                                                                                                                                                                                                                                    gcagcaTCACCTACATTAATGGTCGGCAGCGCCATAACTAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGCTACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTcc       >  2:369831/1‑251 (MQ=255)                                                                                                                                                                                                                                     cagcaTCACCTACATTAATGGTCGAGAGCGCGATAAATAAGGCGCCGACTTTTTGTAGATAATTGTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGGTGGTGATCGCGATACCCGAGGGGAAACCTAAGGAGACGGAAACCGGAGGATGCTCAAGCAGGCTAGCAAAGCGGGCGAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCt      <  1:339274/251‑1 (MQ=255)                                                                                                                                                                                                                                      agcaTCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTg     >  1:315006/1‑251 (MQ=255)                                                                                                                                                                                                                                         aTCACCTACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCGGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCa  <  2:47698/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     GATCCCACGGCAGCACCAGTTGCGGCAGCAGTTGCGGAATACCGTTACCCTGAGAACCATCGGCCAGGACGTAGTGGAATTGCGAACCGATGGTAGCAACATGTCCGCCGAGCAGGTTAACAATCCCCATCACCGCGCAACCAGCCAGCAAGGCCGGAAGGTGACCGGGTAAACGAATGCCCAGACGCGGCCAAAAAACAAGAATACCTAGCGTCACAATGCCAATGGCAGCATCACCCACATTAATGGTCGGCAGCGCCATAAATAATGCGCCGACTTTTTGTAGATAATGTTCCGGGACATGGGCCATTTGCAGACCGAGAAAATCTTTAATCTGCATGGTACCGATGGTGATCCCGATACCCGAGGTGAAACCTAAGGTGACGGAAACCGGAATATACTCAATCAGGCGACCAAAGCGTGCCAGACCCATCAGAATCAAAAAGATCCCCGACAGCAAGGTCGCAACCAGCAGTCCTGCCA  >  NC_000913/1260017‑1260499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑