Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,237,855	C→T	N95N (AAC→AAT)	dadA →	D‑amino acid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,237,855	0	C	T	100.0%	112.7 / NA	36	N95N (AAC→AAT)	dadA	D‑amino acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/13);  total (23/13)

GCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGC  >  NC_000913/1237616‑1238099                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gcCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTCCGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGAAATTTCGACAcc                                                                                                                                                                                                                                             >  1:15747/1‑249 (MQ=255)              aTCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCCAATGTTACGTAATTGCGACACCAGCCACTACATGGa                                                                                                                                                                                                                               >  1:254568/1‑250 (MQ=255)                            gAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCACTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATCTGCTGCACCGTGGGCGGAACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTAGTCTACCCGGTACGCAGTTCCAGTTGAAATGGCTGTTGCAAATGTTACGTACTTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGAt                                                                                                                                                                                                                >  1:223128/1‑251 (MQ=255)                                  aCCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACCCCAGCCACAACCTGGAAAACAAAGGGCGGGTGGTGCg                                                                                                                                                                                                          >  2:81506/1‑251 (MQ=255)                                                    ccGGGGGCCGCCCTGGCAACCAGTGCAGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCCGTTGAAATGGATGTGGCAAATGTTACGTAATTTCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCg                                                                                                                                                                                        >  1:120183/1‑251 (MQ=255)                                                                  ggAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAg                                                                                                                                                                          <  1:332438/251‑1 (MQ=255)                                                                    aaaCCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCa                                                                                                                                                                        <  1:302519/251‑1 (MQ=255)                                                                          gTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCTTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTgcgc                                                                                                                                                                  <  2:255004/251‑1 (MQ=255)                                                                                            aaaTCTCCCCCGGATATGCTGCACCGTGGGCGGAACCAGGTGTGCCTTTAAAAGCGATTCAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGGGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTc                                                                                                                                                  >  1:24015/1‑249 (MQ=255)                                                                                                ctcCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATg                                                                                                                                            >  1:21407/1‑251 (MQ=255)                                                                                                  cccccGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTAt                                                                                                                                             <  1:113171/248‑1 (MQ=255)                                                                                                                 cACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGGGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGAATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGGCAGGGTggg                                                                                                                           >  2:313003/1‑251 (MQ=255)                                                                                                                       gggCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGGACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTTCGTAATTGCGACACCAGCCACTACCTGGAAACCAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGTAGGGCGTCAGGGTGGGACGCTg                                                                                                                     >  2:322402/1‑251 (MQ=255)                                                                                                                             cACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACTAGCCACTACATGGAAAACAAAGGGCGGATGGGGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTg                                                                                                               >  2:260133/1‑251 (MQ=255)                                                                                                                                   gtgtGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCCTTGCGCGCCGAAACCCATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGt                                                                                                         >  1:51422/1‑251 (MQ=255)                                                                                                                                       gCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGGACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGAGGGTGCGTCTGGCGGAAAACAGCCGGGATTGCCTGAAAGCATTGCGCCGCGAAACCAAAATTCAGGATGAAGGGCCGCTGGGATGGACGCTGCCACTGGTCCGTACCg                                                                                                     >  2:122771/1‑251 (MQ=255)                                                                                                                                                aGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTAGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGt                                                                                            <  1:277265/251‑1 (MQ=255)                                                                                                                                                 gCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTa                                                                                           <  1:237687/251‑1 (MQ=255)                                                                                                                                                  cGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCCTTTTGACGGTACGCAGTTCCCGATGAAATGGGTGAGGCAAATGTTAAGTAATTGAGACACCAGCCACTACCTGGAAAACAAAAGGCGGATGGTGCGTCGGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCCGTATGAAGGGCGTCAGGGTGGGACGCTGCACCTGTTCCGCACCGAACAACAGTAt                                                                                          >  1:341409/1‑251 (MQ=255)                                                                                                                                                                 ccccgcgcCAGGCGCCGCGGGGGGTTCGTCTGGACGGTACGCAGTTCCAGTGGAAATGGATGTGGAAAATGTTACGTAATTGGAACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCcgc                                                                           <  2:247628/247‑1 (MQ=255)                                                                                                                                                                  ccAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGAGGTGGCAAATGTTACGAAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCcgcg                                                                          <  2:170781/251‑1 (MQ=255)                                                                                                                                                                   cAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGa                                                                         <  1:275234/251‑1 (MQ=255)                                                                                                                                                                                   tGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATTCAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGa                                                          >  2:103716/1‑250 (MQ=255)                                                                                                                                                                                                    ggTACGCAGTTCCAGTTGAAATGGATGTGGCAACTGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGAGCGTCTTGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGt                                         >  2:163508/1‑250 (MQ=255)                                                                                                                                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTc                                                                                                                                                  >  1:200185/1‑140 (MQ=255)                                                                                                                                                                                                         gCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTc                                                                                                                                                  <  2:200185/140‑1 (MQ=255)                                                                                                                                                                                                                     gAAATGGATGTGGCAAATGTTACGTAATTGCGACACCAGCAACTACATGGAAAACACAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATcc                        >  1:21045/1‑250 (MQ=255)                                                                                                                                                                                                                         gggATGTGGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAgccg                   <  1:54473/250‑1 (MQ=255)                                                                                                                                                                                                                           gATGTGGCAAATGTTCCGTCATTGCGACACCCGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGCATCAGCTGCTGGAATCCAgccgcc                 >  2:220458/1‑251 (MQ=255)                                                                                                                                                                                                                              gtgGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTg               <  1:228106/250‑1 (MQ=255)                                                                                                                                                                                                                               tgGCAAATGTTACGTAATTGCGACACCAGCCCCTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGc             >  1:343046/1‑251 (MQ=255)                                                                                                                                                                                                                               tgGCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGGGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGc             >  1:329819/1‑251 (MQ=255)                                                                                                                                                                                                                                 gaaaaTGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                                                     >  1:306466/3‑193 (MQ=255)                                                                                                                                                                                                                                 gCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                                                     >  1:306465/1‑193 (MQ=255)                                                                                                                                                                                                                                 gCAAATGTTACGTAATTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATc                                                                     <  2:306465/193‑1 (MQ=255)                                                                                                                                                                                                                                          aCGTAATTGCGACACCAGCCACTACATGGAAAACACAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGc  >  2:150851/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GCCTGGTACTTAAATCAGGCAGGACATGAGGTCACCGTCATTGATCGGGAGCCGGGGGCCGCCCTGGAAACCAGTGCCGCGAATGCCGGGCAAATCTCCCCCGGATATGCTGCACCGTGGGCGGCACCAGGTGTGCCTTTAAAAGCGATTAAATGGATGTTCCAGCGCCATGCGCCGCTGGCGGTTCGTCTCGACGGTACGCAGTTCCAGTTGAAATGGATGTGGCAAATGTTACGTAACTGCGACACCAGCCACTACATGGAAAACAAAGGGCGGATGGTGCGTCTGGCGGAATACAGCCGTGATTGCCTGAAAGCATTGCGCGCCGAAACCAATATTCAGTATGAAGGGCGTCAGGGTGGGACGCTGCAACTGTTCCGTACCGAACAACAGTATGAAAATGCGACCCGCGATATCGCCGTGCTGGAAGATGCCGGCGTACCGTATCAGCTGCTGGAATCCAGCCGCCTGGCGGAAGTGGAGC  >  NC_000913/1237616‑1238099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑