Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,231,363	G→A	D60N (GAT→AAT)	umuC →	DNA polymerase V catalytic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,231,363	0	G	A	96.7%	100.1 / NA	30	D60N (GAT→AAT)	umuC	DNA polymerase V catalytic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/13);  minor base C (1/0);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAGATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG  >  NC_000913/1231122‑1231598                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            tCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTtc                                                                                                                                                                                                                                    >  2:378154/1‑251 (MQ=255)    gtagtGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTtcgtc                                                                                                                                                                                                                                 >  1:307849/1‑251 (MQ=255)            gATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGAGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAACATGGGCGATCCCTGGTTCAAACAAAAACATCTGTTTCGTCGCTGTGGc                                                                                                                                                                                                                         >  1:166111/1‑251 (MQ=255)             aTACGCTGGATGTCTTTGGTGTTGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGCCGGTGTTTCGCCCTGATTTATGGGGTTAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCg                                                                                                                                                                                                                        >  2:257216/1‑251 (MQ=255)                   tGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCACACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGttt                                                                                                                                                                                                                  >  2:289303/1‑251 (MQ=255)                    ggATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTGCGTCGCTGTGGCGTGGtt                                                                                                                                                                                                                   <  1:325685/249‑1 (MQ=255)                              ggtgtggtgATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTagcagc                                                                                                                                                                                                       <  2:292312/251‑1 (MQ=255)                                 gtggtgATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAAt                                                                                                                                                                                                    <  2:148026/251‑1 (MQ=255)                                            cgtcgtTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTa                                                                                                                                                                                         <  1:276263/251‑1 (MQ=255)                                                           gcgcTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCa                                                                                                                                                                           >  1:155154/1‑250 (MQ=255)                                                            cgcTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAAt                                                                                                                                                                         >  1:228783/1‑251 (MQ=255)                                                                           tctGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACg                                                                                                                                                          >  2:325159/1‑251 (MQ=255)                                                                            ctGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGAGGTCGAc                                                                                                                                                           <  1:261269/249‑1 (MQ=255)                                                                                gATGTAAACGCGTTTTATGCAAGCTGTGTGACGGTGTTTCGCCCTGATTTCTGGGGTAAACCGGTGGATGTGCTATCGAATAATGACGGTTGCGCTATCGCCCGAAAAGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGAGGTTTGCTTTAACAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGGCGCttg                                                                                                                                                      >  2:228352/1‑248 (MQ=255)                                                                                                                  tgtTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTAtt                                                                                                                   >  1:247141/1‑251 (MQ=255)                                                                                                                                          aaCCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACa                                                                                           <  2:250623/251‑1 (MQ=255)                                                                                                                                          aaCCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACa                                                                                           <  2:155154/251‑1 (MQ=255)                                                                                                                                            ccGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCTTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAgg                                                                                         <  1:373058/251‑1 (MQ=255)                                                                                                                                                     gtgCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGAGTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTa                                                                                 >  2:206366/1‑250 (MQ=255)                                                                                                                                                          aTCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTc                                                                           <  1:23103/251‑1 (MQ=255)                                                                                                                                                               ataatGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGAt                                                                      <  1:158234/251‑1 (MQ=255)                                                                                                                                                                    gACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATctga                                                                  <  1:99518/250‑1 (MQ=255)                                                                                                                                                                            cGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTg                                                         >  1:54411/1‑251 (MQ=255)                                                                                                                                                                                                  gCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGt                                   <  1:259445/251‑1 (MQ=255)                                                                                                                                                                                                      aGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCt                                >  1:340467/1‑250 (MQ=255)                                                                                                                                                                                                                  ttAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGGGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCAt                   >  1:256196/1‑251 (MQ=255)                                                                                                                                                                                                                   tAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATc                  >  2:105102/1‑251 (MQ=255)                                                                                                                                                                                                                   tAAAATGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATc                  >  1:370933/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGGGCGATCCCTGGTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTAc              >  2:68160/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggTTCAAACAAAAAAATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTggg  <  2:126827/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            TCAGTAGTGAAGATACGCTGGATGTCTTTGGTGTGGTGATCCACGTCGTTAAGGCGATGCGCTGATGTTTGCCCTCTGTGATGTAAACGCGTTTTATGCCAGCTGTGAGACGGTGTTTCGCCCTGATTTATGGGGTAAACCGGTGGTTGTGCTATCGAATAATGACGGTTGCGTTATCGCCCGAAACGCTGAGGCAAAGGCGCTTGGCGTTAAAATGGGCGATCCCTGGTTCAAACAAAAAGATCTGTTTCGTCGCTGTGGCGTGGTTTGCTTTAGCAGCAATTATGAGCTTTACGCAGACATGAGCAATCGGGTGATGTCGACGCTGGAAGAGCTATCGCCCCGCGTCGAGATTTACAGTATTGATGAGGCATTCTGCGATCTGACAGGTGTGCGTAATTGTCGCGATCTGACTGATTTTGGCAGAGAAATTCGCGCAACGGTGCTACAACGTACCCATCTTACTGTTGGTGTGGG  >  NC_000913/1231122‑1231598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑