Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	394,482	G→A	intergenic (+64/‑24)	yaiT → / → iprA	putative autotransporter YaiT/putative DNA‑binding transcriptional regulator IprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	394,482	0	G	A	100.0%	94.0 / NA	27	intergenic (+64/‑24)	yaiT/iprA	putative autotransporter YaiT/putative DNA‑binding transcriptional regulator IprA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

ACGGCGATTCCATCGATAACGGTACTGAAGGGTCTGCGGTACGTGTTGGTCTGGGTACTCAGTTTAGCTTCACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCTCCTTATATTATGGGGCTGGCT  >  NC_000913/394249‑394727                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      aCGGCGATTCCATCGATAACGGTACTGAAGGGTCTGCGGTACGTGTTGGTCTGGGTACTCAGTTTAGCTTCACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGAc                                                                                                                                                                                                                                      >  2:39806/1‑251 (MQ=255)         tCCATCGATAACGGTACTGAAGGGTCTGCGGTACGTGTTGGTCTGGGTACTCAGTTTAGCTTCACCAAGAACTTCAGCGCATATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAAGACGGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGATCATTACGGCAAAGGACGCGCaaa                                                                                                                                                                                                                               <  2:18540/250‑1 (MQ=255)                   aCGGTACTGAAGGGTCTGCGGTACGTGTTGGTCTGGGTACTCAGTTTAGCTTCACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTa                                                                                                                                                                                                                    <  1:193404/251‑1 (MQ=255)                                                     gggTACTCAGTTTAGCTTCACCAAGACCTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGAt                                                                                                                                                                                   >  1:325644/1‑250 (MQ=255)                                                                     ttCACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGAt                                                                                                                                                                   >  1:119037/1‑250 (MQ=255)                                                                       cACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACg                                                                                                                                                                <  1:129992/251‑1 (MQ=255)                                                                        aCCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTTGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGTATTGGACGAGCATTACTGCAAAGTACGCGCAAATGTTATCTGTATTTAATCCTCTTCAGGGATTTGGTAAGCGCGATAAATGTTTGTCTAGATACgg                                                                                                                                                               <  2:219687/251‑1 (MQ=255)                                                                         ccAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGt                                                                                                                                                              >  2:246961/1‑251 (MQ=255)                                                                                      cgcCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGt                                                                                                                                                 >  1:137299/1‑251 (MQ=255)                                                                                           ataCCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGGCCAGATACGGTACGCGCTTCGAGTTta                                                                                                                                              <  2:42432/249‑1 (MQ=255)                                                                                                                   gtggtgACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTcc                                                                                                                    >  2:139596/1‑251 (MQ=255)                                                                                                                      gtgACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGt                                                                                                                 >  1:284946/1‑251 (MQ=255)                                                                                                                          cGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTCAATCTACCTGGAAATATTATTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGACCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTACAGTGATg                                                                                                             >  1:165232/1‑251 (MQ=255)                                                                                                                                    gACTGGTCCGCGAACGTGGGTGGTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCGAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCTAGTTTAATAATGAAAAGCAAGTTATATTTTCCCGGGATTTCACTAACGa                                                                                                   <  2:301606/251‑1 (MQ=255)                                                                                                                                     aCTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTAAGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGaggggaagaaagaa                                                                                                  <  1:188975/251‑14 (MQ=255)                                                                                                                                        ggTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAg                                                                                                 <  2:38098/249‑1 (MQ=255)                                                                                                                                         gTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATa                                                                                              <  1:134278/251‑1 (MQ=255)                                                                                                                                                          ttAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTag                                                                               >  2:209318/1‑249 (MQ=255)                                                                                                                                                                                            aaaTACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGCCGAACATTACTGCAAAGGACGCGCAAATGTTATCTGAAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTAc                                           >  1:372107/1‑251 (MQ=255)                                                                                                                                                                                            aaaTACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTAc                                           >  1:110857/1‑251 (MQ=255)                                                                                                                                                                                              aTACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACtt                                         <  2:116840/251‑1 (MQ=255)                                                                                                                                                                                                   ggTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCg                                     <  1:216198/250‑1 (MQ=255)                                                                                                                                                                                                            aTCTAAATAATGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTAccc                            >  1:128632/1‑250 (MQ=255)                                                                                                                                                                                                                   taatGCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCTCCt                    >  1:97356/1‑251 (MQ=255)                                                                                                                                                                                                                       gCCCGTCAAGGATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCGCCTtat                 <  1:139596/250‑1 (MQ=255)                                                                                                                                                                                                                                ggATTTGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCTCCTTATATTATGGggc       >  1:200125/1‑251 (MQ=255)                                                                                                                                                                                                                                     tGACGAGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCTCCTTATATTATGGggctggct  >  1:206182/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      ACGGCGATTCCATCGATAACGGTACTGAAGGGTCTGCGGTACGTGTTGGTCTGGGTACTCAGTTTAGCTTCACCAAGAACTTCAGCGCCTATACCGATGCTAACTACCTCGGTGGTGGTGACGTAGATCAAGACTGGTCCGCGAACGTGGGTGTTAAATATACCTGGTAATATTCTTCACTCCGAAGAAATACTGGTAATTTAATCTAAATAATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTTAAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTCGAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTCGTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACCCAGGCTCCTTATATTATGGGGCTGGCT  >  NC_000913/394249‑394727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑