Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,089,330	G→A	S176S (TCC→TCT)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,089,330	0	G	A	96.7%	93.1 / NA	30	S176S (TCC→TCT)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/11);  minor base C (1/0);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

TCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTT  >  NC_000913/1089097‑1089571                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  tcgcCATAAGGCCAAACAAAAACGTGTGGATTTCCCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTtct                                                                                                                                                                                                                                    <  1:92345/249‑1 (MQ=255)        aaGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACttgt                                                                                                                                                                                                                           >  1:328886/1‑251 (MQ=255)                                                  gCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGcc                                                                                                                                                                                  >  2:131573/1‑250 (MQ=255)                                                                      aCAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGc                                                                                                                                                             >  2:75690/1‑251 (MQ=255)                                                                        aGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTg                                                                                                                                                           <  1:131573/251‑1 (MQ=255)                                                                             ccAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGt                                                                                                                                                       >  1:129741/1‑250 (MQ=255)                                                                               aGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATAGACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATc                                                                                                                                                    <  2:198384/251‑1 (MQ=255)                                                                                   gAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGAcc                                                                                                                                                <  1:71664/251‑1 (MQ=255)                                                                                   gAATTCTTTCCCGGTATTATGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGAcc                                                                                                                                                >  1:138028/1‑251 (MQ=255)                                                                                        cTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGCTTTACATATACAGGCAATAGGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACt                                                                                                                                           >  2:190775/1‑251 (MQ=255)                                                                                               gaaaTTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGATCCGAGAACGCGCACCTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGCCCCAACTGCCGACg                                                                                                                                    >  2:14514/4‑251 (MQ=255)                                                                                                    tCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGc                                                                                                                               >  1:264596/1‑251 (MQ=255)                                                                                                                                       aaaggCACGATTTACATATAAAGGCAATAAGCTGAGGGTGGAATTCGCCTGAATCCCGTAGTGAGAATTCCTTGTAGGGGAAGCGAGCTAAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGTCCTCTGGAAGGCCTGAAGAATTg                                                                                            <  2:210433/247‑1 (MQ=255)                                                                                                                                             aCGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGa                                                                                         >  1:130099/1‑248 (MQ=255)                                                                                                                                              aaacgctgcttttctgatcttaatatGCCGGGGGCATTATCTTGAATAACGTAGGGAGAATTCCATGGATGAGACGCGAGCTAAATGAGCCGAGAACGCGCAACTTCTCGCCCTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTGCTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATCCAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAc                                                                                     <  2:148722/226‑1 (MQ=255)                                                                                                                                                     cATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGt                                                                               >  1:19733/1‑250 (MQ=255)                                                                                                                                                                  tAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAc                                                                                     >  1:148722/1‑231 (MQ=255)                                                                                                                                                                     gCTGCCGGTGGCATTAGGCTGAATAATGTAGTGAGAATTCCATGTATGCGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCAAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTATggg                                                                 >  2:359455/1‑247 (MQ=255)                                                                                                                                                                                 aTTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCaaa                                                    >  1:189542/1‑249 (MQ=255)                                                                                                                                                                                         aaatcctgtattGAGAATTCCAGTTATTAGAACCGAGCTCACCGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTGCTTGTTTATCCTCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTTGAAGGCCTGAAGAATTGGGAAGACGCGTGTATAAAAACTCTGATAGCCTTCATCAAAAGTCAGCACt                                          <  2:211670/240‑1 (MQ=255)                                                                                                                                                                                          aaTACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACt                                          >  2:83430/1‑250 (MQ=255)                                                                                                                                                                                                 tAGTGAGAATCCCATGTGTGGGATGCGAGCTAACCGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTAAGCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCACCTGCCGGCGGGGGCCCATACAGCAGGTGACGGGAATGCTCGAAGAATTGGGAAGACTCGGGTATAAAAACTCTGGTAGCGGTCTTCAAAAGTCACCACGACATCtt                                   <  2:349832/250‑1 (MQ=255)                                                                                                                                                                                                  agtgggaagtcctagTATGAGACGCGAGCTCAACGACCCGAGAACGCGCAACGTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCTACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCAGACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATGAAAAGTCAGCACTACATCtttt                                 <  2:71699/237‑1 (MQ=255)                                                                                                                                                                                                  aGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCTGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCtttt                                 >  2:261233/1‑251 (MQ=255)                                                                                                                                                                                                             cATGTATGAGAAGCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCCCTACAGCTTTTTCCGGAAGCgg                      >  2:318423/1‑251 (MQ=255)                                                                                                                                                                                                                         gCGAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGTTTTTCCTCCTCg          <  1:272338/251‑1 (MQ=255)                                                                                                                                                                                                                           gAGCTCAACGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGAt        >  1:287854/1‑251 (MQ=255)                                                                                                                                                                                                                            aGCTCAACGAGCCGCGAACGCGAAATTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGCTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATTAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACCACAGCTTTTTCCGGAACAGGTTTTACTCCTCGAtg       >  2:73471/1‑251 (MQ=255)                                                                                                                                                                                                                                cAACGAGCCGAGAACGCGCAACTGCGCGCACTTGTTGCCACGGTGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGAACCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGGCATCAAAAGTCAGCACTACAGCGTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGc    <  2:239077/250‑1 (MQ=255)                                                                                                                                                                                                                                 aaCGAGCCGAGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCtt  >  1:226371/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                  TCGCCATAAGGCCAAACAAAAACGTGTGGATTTACCTCAACCTTTGTACGCAGGTATTCCGTCATTTTTACAGCATCCAGACGAATTCTTTCCCGGTATTCTGCTGCGGTTTCATACCGTGCGTGGTCAGTAAAATATGCACGATTTACATATACAGGCAATAAGCTGCCGGTGGCATTAGCCTGAATACCGTAGTGAGAATTCCATGTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTT  >  NC_000913/1089097‑1089571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑