Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,086,957 G→A A297V (GCT→GTT)  pgaC ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,086,9570GA96.9% 104.5 / ‑6.9 32A297V (GCT→GTT) pgaCpoly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (19/12);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTG  >  NC_000913/1086758‑1087196
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
aTGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCAttcat                                                                                                                                                                                                >  2:132049/1‑247 (MQ=255)
        cAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGcac                                                                                                                                                                                       >  1:332274/1‑251 (MQ=255)
            gATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTg                                                                                                                                                                                   <  1:331843/251‑1 (MQ=255)
               atGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGcc                                                                                                                                                                                 <  2:79847/250‑1 (MQ=255)
                         tcaatATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGgc                                                                                                                                                                                                                                      >  1:163701/1‑187 (MQ=255)
                         tcaatATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGgc                                                                                                                                                                                                                                      <  2:163701/187‑1 (MQ=255)
                                           ccGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGAtt                                                                                                                                                    >  1:185639/1‑251 (MQ=255)
                                               cAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAAc                                                                                                                                                >  1:148843/1‑251 (MQ=255)
                                                 aGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTg                                                                                                                                              >  1:96306/1‑251 (MQ=255)
                                                    tGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCa                                                                                                                                            <  2:332274/250‑1 (MQ=255)
                                                         tGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCCGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACACCCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCGCGTAAAAAATCGTCCACTGATTCAACTGCAGCtttc                                                                                                                                      >  2:24970/1‑249 (MQ=255)
                                                           cGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCCTCCACTGATTCAACTGCCGCTTcc                                                                                                                                      >  2:118089/1‑249 (MQ=255)
                                                                  aatGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGGCGCTGTTTCCACATCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGGGGCTCGTAAAAAAGCGTCCACTGATTCAACTGCAGCTTCCAGCTaata                                                                                                                              <  1:232411/250‑1 (MQ=255)
                                                                       aaCCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTaatatcaat                                                                                                                         >  2:34301/1‑250 (MQ=255)
                                                                            aCCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCCCTTATTCAACTCCAGCTTCCCGCTAATCTCAATATCTTc                                                                                                                   >  2:206718/1‑251 (MQ=255)
                                                                             ccAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATTTCTTCg                                                                                                                  >  1:241709/1‑251 (MQ=255)
                                                                                         aGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATct                                                                                                      >  1:75273/1‑250 (MQ=255)
                                                                                                    gtcgtcAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGGTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGt                                                                                           <  2:127947/250‑1 (MQ=255)
                                                                                                          aGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTTCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATCTCGTCACTCC‑AGTAACCCAc                                                                                    >  1:48977/1‑251 (MQ=255)
                                                                                                                 aatCAAAAAACAGCGTCCACATTCGAAAGGTTTCTTTGCGCCACAACCTTGTCATATTTGTGAGGAATACTTCTGCACCGCCCTTAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGGCCACTTATTCAACTGCAGCTTTCTGCTAATATCAAGATCTTGGGTGAGCATATCGTAACTGC‑AGGAACCCACTTcggc                                                                              <  2:355400/248‑4 (MQ=255)
                                                                                                                                   acaTTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAAt                                                           <  1:340799/251‑1 (MQ=255)
                                                                                                                                       tCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCCACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCCCTTCTGCCAGGGCGCTGCGACGAAATgagg                                                       >  2:183476/1‑248 (MQ=255)
                                                                                                                                                  cTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTACCCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCg                                            >  1:210589/1‑251 (MQ=255)
                                                                                                                                                      gcgcCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGaaa                                        <  2:285553/251‑1 (MQ=255)
                                                                                                                                                       cgcCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAAc                                       >  1:117685/1‑251 (MQ=255)
                                                                                                                                                       caccacactcatGTCATATATTTGAGTAATACTTCTGCACCGCCCTGAACACAGAGCAGACGCTGTTTACACAGCACTTTTAACGTTTCAGGCATTAATATCAAGCACAGTGCCCGTGGCTCGTAAAAAATCGTACACTGAATCACATGCAGCTTCCAGCTAACATACATATCTTTGGTGATCATATCGTCACTCCCATGAACCC‑CCTATGCCAGCGCGCTGCGACGAAATTCGGCAATCACACCCGAAAc                                       >  2:42488/12‑251 (MQ=255)
                                                                                                                                                         ccACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACgg                                     <  1:290351/251‑1 (MQ=255)
                                                                                                                                                              aCCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAAt                                <  2:73702/251‑1 (MQ=255)
                                                                                                                                                                                 gAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCTGAAACGGTAAATACGTTTCCATAGATACGCt             <  2:12102/251‑1 (MQ=255)
                                                                                                                                                                                 gAATACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGCAACGGTAAATACGTTTCCATAGATACGCt             >  1:111873/1‑251 (MQ=255)
                                                                                                                                                                                   aTACTTCTGCACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTgg           <  1:243301/251‑1 (MQ=255)
                                                                                                                                                                                            cACCGCCCTGAACCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTg  >  1:241557/1‑251 (MQ=255)
                                                                                                                                                                                                       |                                                                                                                                                                                                                                                
ATGTGTCGCAGCGATATGTGTCAATTCAATATTTAACGGTACACCGGCAAGTTGGACTGCGTAAATAATGAAACCGACCAGGCAGGTGAAGGCCCATATTGTCGTCAGGCAGTATTCAAAAAACAGCGGCCACATTCGAAAGTTTTCTTTGCGCCACAACCTTGTCATATTTTTGAGGAATACTTCTGCACCGCCCTGAGCCCAGCGCAGGCGCTGTTTCCACAGCCCTTTTAACGTTTCAGGCATTAATATCCAGCACAGTGCCCGTGGCTCGTAAAAAATCGTCCACTGATTCAACTGCAGCTTCCAGCTAATATCAATATCTTCGGTGATCATATCGTCACTCC‑AGTAACCCACTTCTGCCAGGGCGCTGCGACGAAATGCGGCAATAACACCGGAAACGGTAAATACGTTTCCATAGATACGCTGGGTTCGCTTG  >  NC_000913/1086758‑1087196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑