Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,331	G→A	G99E (GGG→GAG)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,331	0	G	A	100.0%	71.7 / NA	21	G99E (GGG→GAG)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/10);  total (11/10)

AAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATAAATACGCCGGGAATATCTGA  >  NC_000913/1064107‑1064568                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              aaaTGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCAGTATTTATGAGAATATTGCGTACGAATGATAGACAAATTTATTCAGAGGTTTCAGGAGGTATTACAtatacta                                                                                                                                                                                                                     <  1:25987/251‑1 (MQ=255)         aCGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAatat                                                                                                                                                                                                             >  1:350696/1‑251 (MQ=255)                                              aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCgg                                                                                                                                                                        <  2:364576/251‑1 (MQ=255)                                                           tgtTTTATGCGGTTATACAATGAACTCAGACGAAAAGCAACTGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTc                                                                                                                                                              >  2:180089/1‑248 (MQ=255)                                                                     ggTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATcaca                                                                                                                                                 <  1:141734/251‑1 (MQ=255)                                                                                                  aaTGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACtt                                                                                                                    >  2:281127/1‑251 (MQ=255)                                                                                                                           aaTGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTAGTGGTATAACTTTATGGAGTATATTGAAAACATTAAt                                                                                           <  2:134947/251‑1 (MQ=255)                                                                                                                                           aaaaGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGttt                                                                           <  1:97056/251‑1 (MQ=255)                                                                                                                                             aaGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGttttt                                                                         >  1:319340/1‑251 (MQ=255)                                                                                                                                                                 aatgataatgatCTCTCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGt                                                                                                                                                                                                           >  2:350100/1‑101 (MQ=255)                                                                                                                                                                 aatgataatgatCTATCATTATTTATGAGAATATTGCGTACTAATGAGACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGt                                                                                                                                                                                                           <  1:350100/101‑1 (MQ=255)                                                                                                                                                                      taatgatCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGaa                                                 >  2:86700/1‑250 (MQ=255)                                                                                                                                                                       aatgatCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTGTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGaaa                                                <  2:283587/250‑1 (MQ=255)                                                                                                                                                                          gatCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCagg                                                                                                                                                                                                                                      >  2:157721/1‑65 (MQ=255)                                                                                                                                                                          gatCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCagg                                                                                                                                                                                                                                      <  1:157721/65‑1 (MQ=255)                                                                                                                                                                               aacATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAAtatacgtt                                       >  2:209358/3‑247 (MQ=255)                                                                                                                                                                                      cttatGAGAATATTGCGTACTAATCATACACCACTTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTccagcc                                >  2:25432/2‑251 (MQ=255)                                                                                                                                                                                                 atTGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATa                      >  1:114853/1‑250 (MQ=255)                                                                                                                                                                                                   tGCGTACTAATGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATAAAt                   >  1:221100/1‑251 (MQ=255)                                                                                                                                                                                                            aTGATACACAAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAAGATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGATTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATAAATACGCCGGGa          <  2:266839/251‑1 (MQ=255)                                                                                                                                                                                                                    caAATTTATTCAGAGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATAAATACGCCGGGAATATCTGa  <  1:122724/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              AAATGAGTACGAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAATGATACACAAATTTATTCAGGGGTTTCAGGAGGTATTACATATACTATACAATATGTTCGAGATATTGATATTGTTAGAGTGTCCTTGCCGGGCAGAGCTTCAGAGTCTATCACAGATTTTAAAGGTTATTATTGGTATAACTTTATGGAGTATATTGAAAACATTAATGCGTGTGATGATGTTTTTTCTGAGTATTGTTTTGATGATGAAAATATAAGTGTCCAGCCAGAGCGGATAAATACGCCGGGAATATCTGA  >  NC_000913/1064107‑1064568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑