Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,897	G→A	E426K (GAA→AAA)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,897	0	G	A	100.0%	187.3 / NA	53	E426K (GAA→AAA)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/19);  total (34/19)

TACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCC  >  NC_000913/991655‑992135                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               tACGCACCATGCGCGGATTGCAGTTTGCAGAAGAAGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCg                                                                                                                                                                                                                                        >  1:35136/1‑251 (MQ=255)            cgcgGATTGCAGTTTGCAGAAGACTCCAGCCCGATGGCGCCCCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATTATCCACCCCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGTCGATGAAAGATGCGTCTAATGTCGa                                                                                                                                                                                                                             <  1:300659/251‑1 (MQ=255)               ggATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACACGATCCGACCGGATCTGGTCATTGAGATGAACAACTTCTACACACTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTACAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGAtct                                                                                                                                                                                                                          >  1:46812/1‑251 (MQ=255)                                  aCGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTgg                                                                                                                                                                                                       >  1:328393/1‑251 (MQ=255)                                       aGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTAc                                                                                                                                                                                                    >  1:375302/1‑249 (MQ=255)                                          ccGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGcc                                                                                                                                                                                                >  1:249789/1‑250 (MQ=255)                                               ggCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCCGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCg                                                                                                                                                                                          >  2:279864/1‑251 (MQ=255)                                                 cgcACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACGCCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGt                                                                                                                                                                                        <  1:206721/251‑1 (MQ=255)                                                    ccccGATCCGCCCGGATATGGTCAGTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTaca                                                                                                                                                                                     <  1:135539/250‑1 (MQ=255)                                                               ccGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGCATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGAc                                                                                                                                                                          >  1:334316/1‑251 (MQ=255)                                                                cGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGAcc                                                                                                                                                                         >  2:274547/1‑251 (MQ=255)                                                                  gATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGCCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCAAGCGTCATGATGGTAGAGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAACGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGt                                                                                                                                                                       >  2:334521/1‑251 (MQ=255)                                                                              gagaTGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGAGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAAATTCAAGAAAGGGATGCAGCTTTCTTTAGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCCTTTCCGCCGTTGGTACAGCCAGTCCGGAACACCGATTGTGACCGTCAAAGACGACt                                                                                                                                                            >  1:351478/1‑250 (MQ=255)                                                                                   gAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGCAGTGATTCGCATGATCCACACCCTGCTTGGCGAAAAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAACGACGACTACAATc                                                                                                                                                      >  2:191687/1‑251 (MQ=255)                                                                                        acTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGACTGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGa                                                                                                                                                  >  2:114151/1‑250 (MQ=255)                                                                                              acacCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCg                                                                                                                                             >  1:79492/1‑249 (MQ=255)                                                                                                  ccTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGt                                                                                                                                       >  1:313414/1‑251 (MQ=255)                                                                                                       ccGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACAcc                                                                                                                                  <  1:114151/251‑1 (MQ=255)                                                                                                        cGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACAccc                                                                                                                                 >  1:89167/1‑251 (MQ=255)                                                                                                                 gaaggccggcgAAGTGAATCGAATGCTCCACACCCTGCTTGGCGAAGAAAACTTCCACACAGGGATGCAGCTTTATTTCGAACGTCATGATGATAGTGCAGCGACCTGTGACGACTTTTTGCAGGCGATGAAAGATGCGTCGAATGACGATCTCTCCCATTTCCGCCGGTGGTACAGCCAGTCCGGCACACCGATCGTGACCGTCAAAGACGACTACATTCCGCAAACCGAGCATAACACCCTCACCagcc                                                                                                                        >  2:209113/11‑248 (MQ=255)                                                                                                                 gaAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTGCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATca                                                                                                                        <  2:293637/251‑1 (MQ=255)                                                                                                                  aaGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATcag                                                                                                                       <  1:326934/251‑1 (MQ=255)                                                                                                                  aaGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATc                                                                                                                         >  1:23623/1‑249 (MQ=255)                                                                                                                            aaGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACg                                                                                                             <  2:297967/251‑1 (MQ=255)                                                                                                                                gATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACgcc                                                                                                           >  1:145940/1‑249 (MQ=255)                                                                                                                                           tCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGAt                                                                                              >  1:21488/1‑251 (MQ=255)                                                                                                                                                        tGGCGAAGAAAACTTCCCGAACGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGAAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGt                                                                                                                                       >  1:349487/1‑197 (MQ=255)                                                                                                                                                        tGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTAGTGCGAGCGTCATGATGGGAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTTACCGTCAAAGACGACTACAATCCGGAAAccttgcagt                                                                                                                                       <  2:349487/197‑8 (MQ=255)                                                                                                                                                              agaaAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGc                                                                           >  2:165803/1‑251 (MQ=255)                                                                                                                                                                      tCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCatat                                                                    <  2:249789/250‑1 (MQ=255)                                                                                                                                                                      tCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATAtt                                                                   >  2:18128/1‑251 (MQ=255)                                                                                                                                                                       ccAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTc                                                                  >  1:261362/1‑251 (MQ=255)                                                                                                                                                                          gAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGAGGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGCCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGt                                                               >  2:262133/1‑251 (MQ=255)                                                                                                                                                                              gggATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGCTGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGc                                                           >  2:278646/1‑251 (MQ=255)                                                                                                                                                                               ggATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGCTCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCAAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGcc                                                          >  2:133526/1‑251 (MQ=255)                                                                                                                                                                                         ttATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCTATCTCTCCCATTTCCGCCGTTGGTACAGCCATTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCACAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTg                                                 <  1:284392/250‑1 (MQ=255)                                                                                                                                                                                         ttATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCTCTGCATATTCCGTTTGCCATCGAACTg                                                 <  1:316421/250‑1 (MQ=255)                                                                                                                                                                                         ttATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACt                                                  <  1:88445/249‑1 (MQ=255)                                                                                                                                                                                             tgcGAGCGTCATGAGGGTAGTGCAGCGACCGGTGACGCCTTTGTACGGGCGATGAAAAATGCGTCAAATGTCGACCTTTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATg                                             <  2:72067/248‑1 (MQ=255)                                                                                                                                                                                                 aGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                        <  1:9797/251‑1 (MQ=255)                                                                                                                                                                                                 aGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                                        >  1:301431/1‑251 (MQ=255)                                                                                                                                                                                                    gTCATCATGATAGTGCAGCGACCTCTGCCGAATTTGTGCAGGCGATGACAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTACGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGCTCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGa                                      >  2:219413/1‑250 (MQ=255)                                                                                                                                                                                                        tgatgGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGc                                  >  1:246529/1‑250 (MQ=255)                                                                                                                                                                                                           gggTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAg                              <  2:89167/250‑1 (MQ=255)                                                                                                                                                                                                                gTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATc                         >  1:359344/1‑251 (MQ=255)                                                                                                                                                                                                                  gCAGCGACCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATccc                       >  2:203579/1‑251 (MQ=255)                                                                                                                                                                                                                        aCCTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCatat                                                                    >  1:48072/1‑200 (MQ=255)                                                                                                                                                                                                                        aCCTGTGACGACTTTGGGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCatat                                                                    <  2:48072/200‑1 (MQ=255)                                                                                                                                                                                                                         ccTGTGACGACTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAATCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGc                  >  2:192216/1‑249 (MQ=255)                                                                                                                                                                                                                              gacgacTTTGTGCAGGCGATGAAAGATGCGTCGCATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTACAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGACAgg           >  1:236456/1‑251 (MQ=255)                                                                                                                                                                                                                               ccgacTTTGTGCGGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTCCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGaaatgc          <  2:118311/250‑4 (MQ=255)                                                                                                                                                                                                                               acgacTTTGTGCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGc          <  2:222726/251‑1 (MQ=255)                                                                                                                                                                                                                                       gtgCAGGCGATGAAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATcc  <  2:375302/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               TACGCACCATGCGCGGATTGCAGTTTGCAGAAGACGCCAGCCCGATGGCGCACCCGATCCGCCCGGATATGGTCATTGAGATGAACAACTTCTACACCCTGACCGTTTACGAGAAGGGCGCGGAAGTGATTCGCATGATCCACACCCTGCTTGGCGAAGAAAACTTCCAGAAAGGGATGCAGCTTTATTTCGAGCGTCATGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGCAGAAAGGCGGTCATCC  >  NC_000913/991655‑992135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑