| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 884,185 | G→A | E171E (GAG→GAA) | mdfA → | multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 884,185 | 0 | G | A | 85.3% | 79.6 / ‑0.3 | 34 | E171E (GAG→GAA) | mdfA | multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base G (4/1); new base A (12/17); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTT > NC_000913/883944‑884427 | ggtTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGAtgtt < 1:275237/250‑1 (MQ=255) cACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGGTtgttttgttt < 2:277287/250‑1 (MQ=255) gTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGTCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCg < 2:378232/249‑1 (MQ=255) gCAATAGTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAAAGGGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATACATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCCTTGGc > 1:233073/1‑251 (MQ=255) cAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGGTCCATGTGCTGCCCTGGGGAGGGG‑TGTTTTTTTTGTTTG‑CCGCCTTGGcg > 2:293890/1‑250 (MQ=255) ttGCTGGCGCAAAACATTGAAAAATTCACCCTGTTGCGCTTCTAACAGGGCCCAAGCCTCTGTTTCATTGGCGCTGTGGGATACGACGAAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACAGCGATGATGGCGAACGGGGCGATGAATGATCCGGTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTTGA‑CAGAGATGTTTGTTTTGTTTG‑CCGCATTGGCcgcggt > 1:28585/1‑249 (MQ=255) ggCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGTTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGAtctc < 2:185194/249‑1 (MQ=255) gcAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAAAGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGGGGGCGCGGCGGGGATCCCTGTGCTGCCCTGGGGAGGGG‑TGTTTGTTTTGTTTG‑CCCCATTTGCCGCGATCTCCtttt > 2:268455/1‑251 (MQ=255) gcAAAACATTGAAAAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGATTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGAACCATTTGCTGCCCTGGGGAGGGG‑TGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCtttt > 2:178939/1‑251 (MQ=255) cAAACCATTGAACAATTCACCCGGTTGCGCGTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCttttt < 2:135501/251‑1 (MQ=255) aaaaCATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCGACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTGTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTc < 2:249966/251‑1 (MQ=255) tGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGGCCGCTGGTGGGCGCGGCGTGGATCCATTTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCt < 1:246227/225‑1 (MQ=255) gTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCt < 1:143576/250‑1 (MQ=255) gcgcTTCTTGCAGGGCATAAGCCTCTGTTTCATAGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCCACTTGGTCCGCTGGGGGGCGCGGCGTGGCTCCCTGGTCTGCCCTGGGGAGGGG‑TGTTTGTTTTGTTTT‑CCGCATTGGCAGCGGTCTCCTTTTTCGGGCTGCAACGAGCCATTCCTGaa > 2:148484/1‑250 (MQ=255) aGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCCTTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGt > 1:158313/1‑251 (MQ=255) gggCATACGCCTCTGTTTCATTGGCGCTGCGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCCCGCGt > 2:217733/1‑250 (MQ=255) gggCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑GAGGGATGTTTGTTTTGTTTG‑CCGCCTTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGt > 2:119090/1‑250 (MQ=255) cATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAGCGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATTTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAg < 1:186148/251‑1 (MQ=255) gtgGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGACCGTGGCGCTGATTGCTCCGCTACTTGGTCCGCGGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTGGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCAGGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACt < 2:23551/251‑1 (MQ=255) gggATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCAGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCg < 1:107444/251‑1 (MQ=255) aGGAATCTTTCGAAGGGGCGGTGTGTATCAAGATCCCCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTTCTTGTTCCGCGGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTGGTTTC‑CCGCATTGGCAGCGATCTCCTTTTCCGGTCTGCAACGAGCCATGCCTGAATCCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGGTACTATAAGCTg < 2:225604/251‑1 (MQ=255) gTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTtgtg > 1:87066/1‑251 (MQ=255) gcTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCgggggggggggggg > 1:28890/1‑242 (MQ=255) ggCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGCGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCggggggggggggggcggggg > 2:90957/1‑236 (MQ=255) gCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGCAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCAGCTTTGTGGCgggggggggggggagggggtg > 2:237353/1‑235 (MQ=255) cGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGggcggcgggccggggcttt > 1:333373/1‑236 (MQ=255) gTGGAGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGAggggggggagagggtgggggtgggttt > 2:14682/1‑229 (MQ=255) ggCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGGgctggggctgggggtggttgggt > 2:85546/1‑240 (MQ=255) ccGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGc < 1:50640/251‑1 (MQ=255) ccGCTACTGGGTCCGCTGGTGGGGGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTT‑TTTGTCCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCGAAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCTTGACTATAAGCTGGTGCTGAAGATCGGCCGCGTTGTGGCGGGGGCGCTGGTGCTGGGATTCGTTAGTCTGCCGTTGCTGGc < 1:150610/251‑1 (MQ=255) aCTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AAGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTg < 1:122129/249‑1 (MQ=255) gtgtgCTGCCCTGGG‑AGGGGTTGGTTGTTTTGTTGG‑CCGCATTGGCAGCGTTCTCCTTTTTGGGTCTGCAAGGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACACGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGc < 2:266686/250‑1 (MQ=255) ggcctgggg‑AAGGGAGGTTTGTTTTGTTTG‑CCGTATGGGAAGGGTTCTCCTTTTGCGGCCTGCAACGGGCCATTCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAg < 2:292006/245‑1 (MQ=255) cccTGGG‑AAGGGTTTTTTGTTTTGTTGG‑CCGCATTGGCAGCGATCTCCATTTTCGGTCTGCAACGGGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTTGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGtt < 1:367124/251‑1 (MQ=255) | GGTTTATCGTCACCTGTCTGGCAATATTGCTGGCGCAAAACATTGAACAATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCTTCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTGGGCGCGGCGTGGATCCATGTGCTGCCCTGGG‑AGGGGATGTTTGTTTTGTTTG‑CCGCATTGGCAGCGATCTCCTTTTTCGGTCTGCAACGAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGCTGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGTCTGCCGTTGCTGGCGTGGATCGCCCAGTCGCCGATTATCATCATTACCGGCGAGCAGTT > NC_000913/883944‑884427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |