Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,802	G→A	L227F (CTC→TTC)	ybiO ←	moderate conductance mechanosensitive channel YbiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,802	0	G	A	100.0%	124.2 / NA	35	L227F (CTC→TTC)	ybiO	moderate conductance mechanosensitive channel YbiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

ATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAC‑CAAACACTAATACCG  >  NC_000913/844576‑845047                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       atgatgACGTTCGCCAGCGCACCTATCTGTACATTCCCCTGATTAGAGATAATCGGCCCGGCCACAATCAGGCCATACCCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAAGCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGAt                                                                                                                                                                                                                                <  2:239739/251‑1 (MQ=255)                aGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGActgcctgc                                                                                                                                                                                                                 >  1:27775/1‑251 (MQ=255)                      ccTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTc                                                                                                                                                                                                             >  1:114338/1‑249 (MQ=255)                         aTCTGTCCATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGt                                                                                                                                                                                                         <  1:129474/250‑1 (MQ=255)                                                        gCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAAACGTGAACGGGCGCAGCTCCGCCACGTTTTGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGGTGAAAAACAAACTTTGTGGGAAAGCGATGTTGCGACTGCCTGGATGCAGTTTGTCGCGTAATACCGGTCCGGCAAATAATGTc                                                                                                                                                                         <  2:185973/251‑1 (MQ=255)                                                         cACGGCCACAATCAGGCCCTAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTc                                                                                                                                                                         >  1:116330/1‑250 (MQ=255)                                                             gCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGc                                                                                                                                                                    <  1:304360/251‑1 (MQ=255)                                                                        gCCATAACCTATCAAAATGTTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGAAAAGGCGTTGAACAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACATGGCCGACAAATAATGTAAGGGCCAGTAAcagc                                                                                                                                                          >  2:144755/1‑250 (MQ=255)                                                                                cTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGat                                                                                                                                                  >  1:326799/1‑250 (MQ=255)                                                                                   tCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTACCGGGCGCTGTCGTCCTGAATCGTGCACGGGCGCCGCTCCGCCACGTTTGGGCAAAAACTCAGGCGTAGTACGGCTTTGAACAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGATGTCGCTTACTACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGGCGataat                                                                                                                                               >  1:345790/1‑250 (MQ=255)                                                                                              aaCCATCTCAGGCTACGGCTCCAGTAACTGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTTCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGTCAAAGGCTTTGAAAAACAATCTTTGTTGGAAAGCGATGTGGCGACTTCGTGCATGCAGGTTGTCGCTTAATGCCTGGCCGGCAAATAATGGCAGGGTCTGTAACAGCAGGTCGATAATAAACGCCCCGAt                                                                                                                                   <  1:340057/251‑1 (MQ=255)                                                                                                      cAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTAAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAatcatca                                                                                                                           <  1:26267/251‑2 (MQ=255)                                                                                                      cAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTAAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCg                                                                                                                           <  1:26274/251‑1 (MQ=255)                                                                                                              gacgccAGTAACGGGCGCCCTCTGCATGAATCGGGACCGGGCGCAGTTCCGCCACGTTTGGGAAAAAAAGCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGGTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGGAACAGCAGGTCGATAATAAACGCCCCGATAATTATCGCCGGAAGc                                                                                                                   <  2:301740/247‑1 (MQ=255)                                                                                                               acTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCt                                                                                                                  <  1:82599/251‑1 (MQ=255)                                                                                                                 tCCAGTAACGGGCGCTCTCGTCCTGAATCGAGACCGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAACATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGc                                                                                                                >  2:178109/1‑251 (MQ=255)                                                                                                                           ggCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTtgc                                                                                                      >  2:83914/1‑251 (MQ=255)                                                                                                                            gcgcTCTTGTCCTGAATCGTGAACGGGCGCAGCTCCGACACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCATGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAACCGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTtgct                                                                                                     >  2:26484/1‑251 (MQ=255)                                                                                                                                     tCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACg                                                                                            >  1:311871/1‑251 (MQ=255)                                                                                                                                                             tCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTgccc                                                                    >  1:221782/1‑248 (MQ=255)                                                                                                                                                                 ccACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCAtt                                                                <  2:326799/251‑1 (MQ=255)                                                                                                                                                                    cGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTg                                                             <  2:2705/251‑1 (MQ=255)                                                                                                                                                                            cAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAg                                                     <  1:86984/251‑1 (MQ=255)                                                                                                                                                                              aaaaaTCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCg                                                   <  2:235305/251‑1 (MQ=255)                                                                                                                                                                                 aagcAGGCGTAGTACGGCGTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGGAGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCATGATTTTTTTGCCGCGCCAACTGACCCAGTTTGCGATACAGCGGCa                                                <  2:194564/248‑1 (MQ=255)                                                                                                                                                                                     aGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTgc                                            <  1:87247/251‑1 (MQ=255)                                                                                                                                                                                                 gCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAAt                                >  1:124881/1‑251 (MQ=255)                                                                                                                                                                                                     tGAAAAATTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTGGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGGCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCTCAGGCGAATCAg                             <  2:312606/250‑1 (MQ=255)                                                                                                                                                                                                          aaTTCAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTa                       <  1:139820/251‑1 (MQ=255)                                                                                                                                                                                                              cAATGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACCGCGGCAGTGCGCACAGGCGAATCAGCCAGTaaaa                    >  2:333407/1‑250 (MQ=255)                                                                                                                                                                                                               aaTGAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAc                  >  2:368741/1‑251 (MQ=255)                                                                                                                                                                                                                  gAGGGCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGAGGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCTGAAGCTGCAACCAGTTGCTGCGCGCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAC‑Caa              <  1:23066/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCAAAGGCGTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAC‑CAAac            >  1:199855/1‑250 (MQ=255)                                                                                                                                                                                                                             cGTTGAAAAACAAACATTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGAACAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAAAGCCCCGATAATCATCGCCGGAAGCTGCGACCAGGTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCCGTGCGAACAGGCGAATCAGCCAATAACAACACAAA‑ACTAATAcc   >  2:334825/1‑251 (MQ=255)                                                                                                                                                                                                                              gTTGAAAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAC‑CAAACACTAATACCg  <  2:341964/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ATGATGACGTTCGCCAGCGCACCTATCTGTACATTCACCTGATTAGAGATAATCGGCACGGCCACAATCAGGCCATAACCTATCAAACTGCTTAACCAGCTCAGGCGACGACTCCAGTAACGGGCGCTCTCGTCCTGAATCGTGAACGGGCGCAGCTCCGCCACGTTTGGGCAAAAAATCAGGCGTAGTACGGCTTTGAAAAATTCAATGAGGGCAAAGGCGTTGAGAAACAAACTTTGTTGGAAAGCGATGGTGCGACTGCCTGCATTCAGGTTGTCGCTTAATACCTGGCCGACAAATAATGTCAGGGCCAGTAACAGCAGGTCGATAATAAACGCCCCGATAATCATCGCCGGAAGCTGCAACCAGTTGCTGCGCTCACGATTTTTTTGCCGCGCCCACTGACCCATTTTGCGATACAGCGGCAGTGCGCACAGGCGAATCAGCCAGTAAAAAC‑CAAACACTAATACCG  >  NC_000913/844576‑845047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑