Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	787,088	G→A	L170L (CTG→TTG)	gpmA ←	2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	787,088	0	G	A	100.0%	142.9 / NA	40	L170L (CTG→TTG)	gpmA	2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

CTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGT  >  NC_000913/786846‑787333                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      cTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGttt                                                                                                                                                                                                                                               >  1:11271/1‑251 (MQ=255)                        ccgccgCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCgg                                                                                                                                                                                                                        >  2:40805/1‑251 (MQ=255)                                      gCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAgcgc                                                                                                                                                                                                          >  1:98888/1‑251 (MQ=255)                                                cGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTcc                                                                                                                                                                                                <  1:16213/251‑1 (MQ=255)                                                 gTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCATGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATCTCACCAGCGCACGTAAAGAGTTAACGTGTGCAGAGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCg                                                                                                                                                                                               >  2:265332/1‑251 (MQ=255)                                                 gTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATCTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCg                                                                                                                                                                                               >  2:265322/1‑251 (MQ=255)                                                      cATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGc                                                                                                                                                                                          <  1:223864/251‑1 (MQ=255)                                                      cATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGc                                                                                                                                                                                          <  1:52189/251‑1 (MQ=255)                                                         tACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGc                                                                                                                                                                                       >  2:220438/1‑251 (MQ=255)                                                          aCCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGc                                                                                                                                                                                       <  2:270634/250‑1 (MQ=255)                                                           cccAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGc                                                                                                                                                                                       >  2:373106/1‑249 (MQ=255)                                                                         tttCAGCGGTTTGAAATTCTCGTCGAACTCATACACCCGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTc                                                                                                                                                                        >  2:193616/1‑250 (MQ=255)                                                                                        aTTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAAcgcg                                                                                                                                                        <  2:140585/251‑1 (MQ=255)                                                                                           ctcGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGAt                                                                                                                                                     >  2:234684/1‑251 (MQ=255)                                                                                                      cATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCACTTCATACGCGGCAAAATAGTTTCATCCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCgta                                                                                                                                          <  2:257769/251‑3 (MQ=255)                                                                                                                         aGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCGTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAg                                                                                                                       <  1:348662/251‑1 (MQ=255)                                                                                                                            cGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTc                                                                                                                     >  1:4559/1‑250 (MQ=255)                                                                                                                                atatTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTc                                                                                                                >  1:18999/1‑251 (MQ=255)                                                                                                                                        cTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCcggcgg                                                                                                          >  1:365904/1‑249 (MQ=255)                                                                                                                                                   cttcttCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCaa                                                                                               <  1:47705/249‑1 (MQ=255)                                                                                                                                                        tcGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCacg                                                                                        >  1:316392/1‑251 (MQ=255)                                                                                                                                                                gTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGtt                                                                                                                                                                                   >  1:93615/1‑152 (MQ=255)                                                                                                                                                                gTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGtt                                                                                                                                                                                   <  2:93615/152‑1 (MQ=255)                                                                                                                                                                 ttATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCCCTGCAAAACCACGACGCCACTg                                                                               >  2:86993/1‑251 (MQ=255)                                                                                                                                                                   aTCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGtt                                                                             <  2:18999/251‑1 (MQ=255)                                                                                                                                                                           atTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGc                                                                     >  2:377946/1‑251 (MQ=255)                                                                                                                                                                             ttCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCt                                                                    <  1:313386/250‑1 (MQ=255)                                                                                                                                                                                        aCGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACt                                                        >  2:16199/1‑251 (MQ=255)                                                                                                                                                                                            aaaGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACtttt                                                     <  2:317725/250‑1 (MQ=255)                                                                                                                                                                                               gagTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTcagc                                                 >  1:149796/1‑251 (MQ=255)                                                                                                                                                                                                  ttACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTCACGCGGATCGGGACCAGGATCACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCAACTGCTCGTCGCCATACTTTTCAGCAGt                                              >  2:327161/1‑251 (MQ=255)                                                                                                                                                                                                       gtgtGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCg                                         <  2:207480/251‑1 (MQ=255)                                                                                                                                                                                                         gtgCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTTCAAAACCACGACGCCACTGTTTCACCTGCTCGTCTCCATACTTTTCAGCAGTTTCCGCt                                       <  1:254172/251‑1 (MQ=255)                                                                                                                                                                                                                      cACGCGCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCt                          <  1:86421/251‑1 (MQ=255)                                                                                                                                                                                                                        cgcgCTCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCTGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCTGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGc                        <  2:266156/251‑1 (MQ=255)                                                                                                                                                                                                                             tCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCCCGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGc                   >  2:303988/1‑251 (MQ=255)                                                                                                                                                                                                                             tCACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGc                   >  1:161666/1‑251 (MQ=255)                                                                                                                                                                                                                              cACCGCTCTTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACg                    <  2:120871/249‑1 (MQ=255)                                                                                                                                                                                                                                     ctTCATACGCGGCAAAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTa             <  1:123506/249‑1 (MQ=255)                                                                                                                                                                                                                                              cgGCAAAATCGTTTCCTTCAAGTAAAGGATCACGCGATCAATGGTCAGCGCAAGGCTTTCCATCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGACCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGAcgttcgt  >  2:181986/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CTTCGCTTTACCCTGGTTTGCAACCGCCGCTGCTTTCGCTGCGATCTCGTCAGCATTACCCAGATAATAGCGTTTCAGCGGTTTGAAATTCTCGTCGAACTCATACACCAGCGGCACGCCAGTCGGGATATTAAGCTCAAGAATCTCTTCTTCGCTCATGTTATCAAGATATTTCACCAGCGCACGTAAAGAGTTACCGTGTGCAGCGATGATCACGCGCTCACCGCTCTTCATACGCGGCAGAATAGTTTCATTCCAGTAAGGGATCACGCGGTCAATGGTCAGCGCCAGGCTTTCCGTCAGCGGCAGTTCTTTCTCGCTCAGTTTCGCGTAACGCGGATCGTGACCCGGATAACGCTCATCATCTTTAGTCAGTTCCGGCGGAGTCACTGCAAAACCACGACGCCACTGTTTCACCTGCTCGTCGCCATACTTTTCAGCAGTTTCCGCTTTGTTCAGACCCTGCAACGCACCGTAGTGACGTTCGT  >  NC_000913/786846‑787333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑