Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	752,785	C→T	G4E (GGA→GAA)	ybgD ←	putative fimbrial protein YbgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	752,785	0	C	T	100.0%	52.5 / NA	16	G4E (GGA→GAA)	ybgD	putative fimbrial protein YbgD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

AGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTCCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTAT  >  NC_000913/752555‑753028                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTcc                                                                                                                                                                                                                                 <  2:55892/251‑1 (MQ=255)          aGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGat                                                                                                                                                                                                                        <  2:281741/251‑1 (MQ=255)                           aCTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAg                                                                                                                                                                                                       <  1:81787/251‑1 (MQ=255)                                   gCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATGTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCTGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGcc                                                                                                                                                                                                <  2:214651/250‑1 (MQ=255)                                              ccctgTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTAt                                                                                                                                                                                    <  2:115317/247‑1 (MQ=255)                                                           ggTTGTCGTGAATTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCt                                                                                                                                                                        >  2:8370/1‑250 (MQ=255)                                                                   tGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCtagttttt                                                                                                                                                                 >  1:237760/1‑249 (MQ=255)                                                                                                       ctGGATGCATTTCACACGGTGCTCCAATAACATCCCCCTTAAAGTGAATTTCGCCCGACCCTTCCTCCGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGCATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAACTATTGTTAGTTTTATTAAATGc                                                                                                                            >  2:43420/1‑250 (MQ=255)                                                                                                          gATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTCAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCaaa                                                                                                                         >  1:16835/1‑250 (MQ=255)                                                                                                                 ttCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAat                                                                                                                  <  1:357837/250‑1 (MQ=255)                                                                                                                                                   tGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATaa                                                                                >  1:154066/1‑250 (MQ=255)                                                                                                                                                      aTTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTGTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTGTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAata                                                                            <  1:142704/251‑1 (MQ=255)                                                                                                                                                                               caTAAACAGGGGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCAtt                                                   >  2:222169/1‑251 (MQ=255)                                                                                                                                                                                                                    tGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAACTTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGttttt              >  2:317190/1‑251 (MQ=255)                                                                                                                                                                                                                    tGCGGCCAATGTTTTTTGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGttttt              >  1:53261/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttttGTTCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTAt  <  1:65586/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTCATCAGCAGCATAAACAGGTGCAGTGAACAGCAGAGATACGGCCAGTGCGGCCAATGTTTTTTGTCCTTTAAACATAACAGAGTCCTTTAAGGATATAGAATAGGGGTATAGCTACGCCAGAATATCGTATTTGATTATTGCTAGTTTTTAGTTTTGCTTAAAAATATTGTTAGTTTTATTAAATGCAAAACTAAATTATTGGTATCATGAATTTGTTGTATGATGAATAAAATATAGGGGGGTATAGATAGACGTCATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTAT  >  NC_000913/752555‑753028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑