Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	637,484	T→C	L45L (TTA→TTG)	ybdO ←	putative LysR family DNA‑binding transcriptional regulator YbdO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	637,484	0	T	C	100.0%	128.1 / NA	35	L45L (TTA→TTG)	ybdO	putative LysR family DNA‑binding transcriptional regulator YbdO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/20);  total (15/20)

CTTGCCGTAGGCAGCGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGTATATATTTTTATTGATTATGTTTTTTTGCTTAACTCAGGATTAAGTTTTTC  >  NC_000913/637252‑637729                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      cTTGCCGTAGGCAGCGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTAGGACTTATTGACTCTATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCt                                                                                                                                                                                                                                     >  1:365109/1‑251 (MQ=255)      cGTAGGCAGCGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATgg                                                                                                                                                                                                                                >  2:33317/1‑251 (MQ=255)        tAGGCAGCGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATgggg                                                                                                                                                                                                                              >  1:283354/1‑251 (MQ=255)               cGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGatata                                                                                                                                                                                                                       <  1:110650/251‑1 (MQ=255)                ggATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGatatat                                                                                                                                                                                                                      >  2:284918/1‑251 (MQ=255)                             attattAGAGCAAGAAATGAGCTGAGGGCCGGAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTTTTCAAGGCCTCTGAGATTGTGCTCAAGATGGTGATGCATATTTAACCCTGTTGTCGTCGGCGCGATACCTGTTCCTGAGCGAATGAATAAAGGGTCATTAAAGTGCGTACGTAAGCGTTGCAACGACTGGCTCACTTCTGATGGGGTGATATATAGTGATTcagca                                                                                                                                                                                                          <  2:343254/250‑1 (MQ=255)                                        aaGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATg                                                                                                                                                                                              <  2:79152/251‑1 (MQ=255)                                           aaaTGAGCTGAGGGCCGTCGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCtt                                                                                                                                                                                           >  1:279824/1‑251 (MQ=255)                                                    gAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTg                                                                                                                                                                                   <  2:67433/250‑1 (MQ=255)                                                    gAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGAATTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCTGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTg                                                                                                                                                                                   <  1:559/250‑1 (MQ=255)                                                                    aaaGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGc                                                                                                                                                                                                                                             >  1:257074/1‑176 (MQ=255)                                                                    aaaGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGc                                                                                                                                                                                                                                      <  1:235386/183‑1 (MQ=255)                                                                    aaaGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGc                                                                                                                                                                                                                                      >  2:235386/1‑183 (MQ=255)                                                                    aaaGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGACCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGc                                                                                                                                                                                                                                             <  2:257074/176‑1 (MQ=255)                                                                      aGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATg                                                                                                                                                                <  2:285887/251‑1 (MQ=255)                                                                                 aGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGAtt                                                                                                                                                     >  2:74288/1‑251 (MQ=255)                                                                                            aTTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGAGGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACTCTCAAATATGACAAGAAGATTTAAGTCGAACt                                                                                                                                          <  2:322257/251‑1 (MQ=255)                                                                                                      ttAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTc                                                                                                                                >  2:121064/1‑251 (MQ=255)                                                                                                            gtttgttCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTagag                                                                                                                          <  1:366550/251‑1 (MQ=255)                                                                                                            gtttgttCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTaga                                                                                                                           <  2:101677/250‑1 (MQ=255)                                                                                                                              agaTTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTc                                                                                                        <  1:126758/251‑1 (MQ=255)                                                                                                                                  ttttCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGACGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGt                                                                                                    >  1:8605/1‑251 (MQ=255)                                                                                                                                           gATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTTAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATAttttt                                                                                           <  2:192088/251‑1 (MQ=255)                                                                                                                                                          ttAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGatatat                                                                             <  1:330505/250‑1 (MQ=255)                                                                                                                                                                  gttgtCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAAt                                                                    <  1:86340/251‑1 (MQ=255)                                                                                                                                                                     gtcgtcGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGGACGTAAGCGTTGCAACGACTGGCGCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTGGCTAATGCTTAAATGCTGGTAAATACACTCAAATAGGACAAGAAGATTTAAGGCGAACTTTTTCAAGTCGTAGATATTGGTCATACACTACTCCGGGTATATTTCTCTTCCATGATATAGTCAGTTAATct                                                                  <  2:237858/250‑2 (MQ=255)                                                                                                                                                                        gtcgGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTa                                                                      <  2:145863/243‑1 (MQ=255)                                                                                                                                                                        gtcgGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTa                                                                      >  1:145863/1‑243 (MQ=255)                                                                                                                                                                           ggCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGtttt                                                           <  1:137343/251‑1 (MQ=255)                                                                                                                                                                               cgATACCTTTTCCTGAGCGAATGCATAAAGGGTCCTTAAATTGCGCACGTAAGAGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGc                                                       >  1:339076/1‑251 (MQ=255)                                                                                                                                                                                   aCCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGtat                                                   >  1:266864/1‑251 (MQ=255)                                                                                                                                                                                          ccTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGTATATAttt                                             <  1:325574/250‑1 (MQ=255)                                                                                                                                                                                                         aaaGGGTCATTAAATTGCGCACGTAAGCGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGTATATATTTTTATTGATTATGttt                              <  1:145956/250‑1 (MQ=255)                                                                                                                                                                                                                    aaaTTGCGCACGTCTGCGTTGCAACGACTGGCTCACGACTGATGGGGAGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGCCAAGAAGATTTAAGTCGAACTTTTTCAAGGCGTAGCGATTGGCCATACTCTATTCCGGTTATCTTTTTCTTACATGATATATTCACTTAATCAATGTTTTTTGCGTATATATTTTTATTGATTATGTTTTTTTGCTTAACt                  >  2:342999/1‑251 (MQ=255)                                                                                                                                                                                                                                    cGTTGCAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGTATATATTTTTATTGATTATGTTTTTTTGCTTAACTCAGGATTAAGTTTTTc  >  1:160985/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CTTGCCGTAGGCAGCGGATCAGCATACTATTATTAGAGCAAGAAATGAGCTGAGGGCCGTAGATAATAAAGTTTTTCTTTAGTTCGGACTTATTGACTATATTAATTGTTTGTTCAAGGCCTCTGAGATTTTTCTCAAGATGGTGATGCAGATTTAACCCTGTTGTCGTCGGCGCGATACCTTTTCCTGAGCGAATGAATAAAGGGTCATTAAATTGCGCACGTAAGCGTTGTAACGACTGGCTCACGGCTGATGGGGTGATATATAGTGATTCAGCAGCTTTGCTAATGCTTAAATGCTGGTAAATACACTCAAATATGACAAGAAGATTTAAGTCGAACTTTTTCAAGTCGTAGAGATTGGCCATACACTACTCCGGTTATATTTTTCTTCCATGATATATTCACTTAATCAATGTTTTTTGCGTATATATTTTTATTGATTATGTTTTTTTGCTTAACTCAGGATTAAGTTTTTC  >  NC_000913/637252‑637729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑