Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	625,977	G→A	V365M (GTG→ATG)	entC →	isochorismate synthase EntC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	625,977	0	G	A	100.0%	175.6 / NA	49	V365M (GTG→ATG)	entC	isochorismate synthase EntC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/27);  total (22/27)

GTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTT  >  NC_000913/625732‑626220                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTTCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCt                                                                                                                                                                                                                                                <  2:131034/251‑1 (MQ=255)       cGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGcgtcg                                                                                                                                                                                                                                          <  2:206117/251‑1 (MQ=255)          aTTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCgtcgtc                                                                                                                                                                                                                                        >  1:127360/1‑250 (MQ=255)                     aaCGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTg                                                                                                                                                                                                                             >  2:24900/1‑250 (MQ=255)                             gACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCCGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGcgcg                                                                                                                                                                                                                    >  2:319929/1‑251 (MQ=255)                                 ctGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTGCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTGTGGCGTCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGaa                                                                                                                                                                                                                  <  2:113111/249‑1 (MQ=255)                                        gTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGt                                                                                                                                                                                                          <  1:190496/250‑1 (MQ=255)                                        gTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGt                                                                                                                                                                                                          <  1:96246/250‑1 (MQ=255)                                        gTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTc                                                                                                                                                                                                         <  1:370482/251‑1 (MQ=255)                                        gTCTGCTGCATCCGACCCCCGCGCGGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGGGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTc                                                                                                                                                                                                         <  1:349143/251‑1 (MQ=255)                                            gctgcATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAAc                                                                                                                                                                                                     <  1:10726/251‑1 (MQ=255)                                              tgcATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACtt                                                                                                                                                                                                   >  1:270142/1‑251 (MQ=255)                                                   ccGACCCCCGCGCTGAGCGGTTGCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCTTTCGACCGCGAACTGTGTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCAGCTGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTa                                                                                                                                                                                               <  1:369067/250‑1 (MQ=255)                                                      ccccccTCGCTGAGCGGTTTCCCGCATCCGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTGGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACATGCGTCAAACTGTCTACCAt                                                                                                                                                                                           <  2:227162/250‑1 (MQ=255)                                                        ccccGCGCTGAGCGGTTTCCCGCATCCGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGCCCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGt                                                                                                                                                                                         >  1:339045/1‑251 (MQ=255)                                                             cgcTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAAc                                                                                                                                                                                    >  1:162937/1‑251 (MQ=255)                                                                 gAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGt                                                                                                                                                                                  <  1:203818/249‑1 (MQ=255)                                                                           ccGCATCAGGCCGCGACCCAGGTTATTGCGGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTGCCATGTTGACCGTTTTTGGATTGCa                                                                                                                                                                      <  2:162937/251‑1 (MQ=255)                                                                                tCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTa                                                                                                                                                                   >  1:309809/1‑249 (MQ=255)                                                                                       gcgACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAg                                                                                                                                                          <  1:225861/251‑1 (MQ=255)                                                                                             cAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAg                                                                                                                                                    <  1:320653/251‑1 (MQ=255)                                                                                                 ttATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGcatt                                                                                                                                                >  1:57958/1‑251 (MQ=255)                                                                                                        tGAACTGGAACCGTTCGACCGCGAACTGTTTGGAGGCCTTGTGAGCTGGTGTGACAGCGCAGGTAACGGCGAAAGGGTGGTGCCCACCCGCTGCGCGAAGCTGAGGGAAAATCAGGTGAGTCTGTATGCCGGACAGGGGATTATGCCTGCGTCGTAACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTATACCATGTTGACCGTTTTTGGATTGCATTAAGGACCGAGGATGAGcattccattc                                                                                                                                          >  1:344786/1‑250 (MQ=255)                                                                                                          aaCTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCAcc                                                                                                                                       <  1:87143/251‑1 (MQ=255)                                                                                                              ggAACCGTGCGACCGCGAACTGTTTGGCGGGATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCTAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCTCCCGCt                                                                                                                                   <  2:112968/251‑1 (MQ=255)                                                                                                                aaCCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCCTCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTgg                                                                                                                                 >  1:61936/1‑251 (MQ=255)                                                                                                                        gACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGa                                                                                                                            >  1:77542/1‑248 (MQ=255)                                                                                                                                   gTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCg                                                                                                                <  1:352768/249‑1 (MQ=255)                                                                                                                                     ttGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGAGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCg                                                                                                             <  2:239654/250‑1 (MQ=255)                                                                                                                                     ttGGCGGCATTGTGGGTTGGGGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGCTGCGCATTCCATTCACCCGCTGGCCGGAAGGGTTTGCCCGTCGc                                                                                                            >  2:79982/1‑251 (MQ=255)                                                                                                                                       ggcggcATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTa                                                                                                          <  1:53053/251‑1 (MQ=255)                                                                                                                                             aTTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCggg                                                                                                     >  2:54508/1‑250 (MQ=255)                                                                                                                                                  gggTTGGCGTGCCAGCGAACGTAACGGAGAATCGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAACTCAGGAGCGTCTGCTTGCCGGAGCGGGGATTATGACTGCGTCGTCACCGTTGGGAGAGTGGCGCGAAACAGGCGTCACACTTTCTACCATGTTGAACGTTTTTGGCTTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGCGTTTGCCAGTCGCTATccggcaac                                                                                                 >  2:238691/1‑242 (MQ=255)                                                                                                                                                         ggtgACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTg                                                                                        <  2:49210/250‑1 (MQ=255)                                                                                                                                                          gtgACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTg                                                                                        <  2:347721/250‑1 (MQ=255)                                                                                                                                                                gtgAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGAt                                                                                 <  2:281377/249‑1 (MQ=255)                                                                                                                                                                  gAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGAttt                                                                               >  1:342429/1‑251 (MQ=255)                                                                                                                                                                   aaGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTg                                                                              >  1:194112/1‑251 (MQ=255)                                                                                                                                                                                      ggtgACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTAGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTg                                                            <  2:265808/250‑1 (MQ=255)                                                                                                                                                                                             aTCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGAc                                                     >  2:41017/1‑250 (MQ=255)                                                                                                                                                                                                  cTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGa                                                                                                                            >  1:115435/1‑174 (MQ=255)                                                                                                                                                                                                  cTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGa                                                                                                                            <  2:115435/174‑1 (MQ=255)                                                                                                                                                                                                    gcgcGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACAtgctgc                                              <  1:167863/250‑1 (MQ=255)                                                                                                                                                                                                                              cGTCTGTTTGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACgg                   <  1:317406/251‑1 (MQ=255)                                                                                                                                                                                                                                     ttGCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGgcgagcg             >  2:97795/1‑250 (MQ=255)                                                                                                                                                                                                                                       gCCGGAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGGGACAGCATCGCGGTTATCGACGGCGAGCGAc           >  2:185261/1‑250 (MQ=255)                                                                                                                                                                                                                                           gAGCGGGGATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTg      <  2:179382/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggggATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGtt  >  1:360757/1‑251 (MQ=255)                                                                                                                                                                                                                                                gggATTATGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGtt  >  2:71328/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCGACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAACCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGCGAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCCGGAGCGGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAACGTTTTTGGATTGCATTAAGGAGCGAGGATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCTGACCGACATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTT  >  NC_000913/625732‑626220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑