Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	488,145	C→T	A537V (GCT→GTT)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	488,145	0	C	T	100.0%	95.0 / NA	29	A537V (GCT→GTT)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/16);  total (13/16)

CCTGACTCAGCAAATCTTTTGGGTGAACAGTAACC‑GTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGGTGTTTGGCCTGTGCTGGAAGGTACTGGAGAAAAACGGC  >  NC_000913/487906‑488365                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             ccggACTCAGCAAATCTTTGGGGTGAACAGTACCC‑GTCCAATGGACTGGGACTGGATCAAAGCGTCCCCGCAAAGCCTGAAAGAGGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTccc                                                                                                                                                                                                                   <  1:206184/248‑1 (MQ=255)   tGACTCAGCAAATCTTTTGGGTGAACAGTAACC‑GTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTg                                                                                                                                                                                                                 >  1:267132/1‑251 (MQ=255)       ccAGCAAATTTTTTGGGTGAACAGTAACC‑GGCCAAGGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGATTTTAAGTCGATAAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGTTTCCCTGCGTa                                                                                                                                                                                                             <  1:274409/250‑1 (MQ=255)        cAGCAAATTTTTTGGGTGAACA‑TAACCCGTCCAAGGGACTGGGACTGGATCAAAGCGTTCCCGAAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACGGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGt                                                                                                                                                                                                              <  1:348300/249‑1 (MQ=255)                 ttttGGGTAAAAAGAAACC‑GTCCAATGGATGGGGACTGGATCAAGGCGTTCCCGAAAAGCCTGAAAGATGAATTAAGGTCAAGAAAAATCACGGTAAACGGGAAAAAAGCCGGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGAcagcc                                                                                                                                                                                                    <  2:352998/250‑1 (MQ=255)                               tAACC‑GTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCa                                                                                                                                                                                      >  1:65953/1‑250 (MQ=255)                                              actgggactggATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATc                                                                                                                                                                       >  2:286475/1‑251 (MQ=255)                                                         aTCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCg                                                                                                                                                            >  2:246934/1‑251 (MQ=255)                                                                                    gATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATtct                                                                                                                                 >  1:126887/1‑251 (MQ=255)                                                                                            tAAGTCGATGAAAATCACGGTGAACTGGCAAAACGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGCGTGCCTGATTATTCTCGCGGTTg                                                                                                                         >  2:50302/1‑251 (MQ=255)                                                                                                gagaggaaagtcgcggagaacggaaaaaagGCCTGTCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCt                                                                                                                     <  2:238255/226‑1 (MQ=255)                                                                                                            agggTGAACGGGAAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGAc                                                                                                         <  1:362586/249‑1 (MQ=255)                                                                                                              ggtaaacgggCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCa                                                                                                       <  1:142325/243‑1 (MQ=255)                                                                                                                           aaaGCCGGGCCCGCCGTTTTTATCGCTTGCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACAt                                                                                          <  1:159813/251‑1 (MQ=255)                                                                                                                                    cccGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACt                                                                                 <  1:360354/251‑1 (MQ=255)                                                                                                                                      cgccgTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGc                                                                               >  1:189759/1‑251 (MQ=255)                                                                                                                                              ttATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCg                                                                       >  1:75749/1‑251 (MQ=255)                                                                                                                                               tATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGt                                                                      >  1:183912/1‑251 (MQ=255)                                                                                                                                                      ttCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCa                                                                >  2:128374/1‑250 (MQ=255)                                                                                                                                                        ccTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCaaaa                                                             <  2:183912/251‑1 (MQ=255)                                                                                                                                                           cGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCaaaaaa                                                           <  1:170179/250‑1 (MQ=255)                                                                                                                                                           cGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATGATTCGCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAAc                                                          <  2:179780/251‑1 (MQ=255)                                                                                                                                                                    ggcGCTGCTGTTGATTGTCGGGCTGTTCCACTGGCGTCTGGGCTGGCTGAAAGCGTAGCAACAAAAACTGGTTTCCTTTGTTGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGGGTGCCTGATTATTCTCGCGTTTGGGCTGAGTTGGTTGTCCATGCGGCTCAACATCATTGAACTGCTATGGTCGCTGAGCaaaaggcttgcgata                                                  <  2:171644/248‑12 (MQ=255)                                                                                                                                                                     ccGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATAtt                                                <  1:355727/251‑1 (MQ=255)                                                                                                                                                                          gctgTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTctgg                                            <  2:280570/250‑1 (MQ=255)                                                                                                                                                                              ttGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGgt                                       >  1:115431/1‑251 (MQ=255)                                                                                                                                                                                                    ggCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGGTGTTTGGCCTGTGCTGGAAGGTa                 <  2:150881/251‑1 (MQ=255)                                                                                                                                                                                                            ggctggctGAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGGTGTTTGGCCTGTGCTGGAAGGTACTGgaga          >  1:279326/1‑250 (MQ=255)                                                                                                                                                                                                                   tgAAAGCGTATCAACAAAAACTGGCTTCCGTTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGGTGTTTGGCCTGTGCTGGAAGGTACTGGAGAAAAACGGc  >  1:9477/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                             CCTGACTCAGCAAATCTTTTGGGTGAACAGTAACC‑GTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGGCAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTGGCGTCTGGGCTGGCTGAAAGCGTATCAACAAAAACTGGCTTCCGCTGTGGGTTCCCTGCGTAACGACAGCCAGCTCAACACACCAAAAGCGATCCTTATCGACCTGATCCGTGCGCTGCCGGTGTGCCTGATTATTCTCGCGGTTGGCCTGATTCTGTTGACCATGCAGCTCAACATCAGCGAACTGCTATGGTCGTTCAGCAAAAAACTGGCGATATTCTGGCTGGTGTTTGGCCTGTGCTGGAAGGTACTGGAGAAAAACGGC  >  NC_000913/487906‑488365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑