Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,002	C→T	A182T (GCA→ACA)	ampG ←	muropeptide:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,002	0	C	T	100.0%	131.3 / NA	38	A182T (GCA→ACA)	ampG	muropeptide:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/14);  total (24/14)

ACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTC  >  NC_000913/452761‑453242                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 aCGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTaa                                                                                                                                                                                                                                         <  2:363375/251‑1 (MQ=255) aCGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTa                                                                                                                                                                                                                                          >  2:188967/1‑250 (MQ=255)       acttcaccagaacccaacacgtcgccccGAATCCCAAACGTGGTTGTCAGGCTCATGGCGCATGCGTCGCCCACCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGt                                                                                                                                                                                                                                    >  2:166230/23‑250 (MQ=255)            aCCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGc                                                                                                                                                                                                                              <  1:205154/251‑1 (MQ=255)              ccGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGccc                                                                                                                                                                                                                            >  2:308293/1‑251 (MQ=255)                                   gAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCt                                                                                                                                                                                                       >  1:166544/1‑251 (MQ=255)                                                   tGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCATCCACAGGGCt                                                                                                                                                                                        <  1:118863/250‑1 (MQ=255)                                                    gtctggCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAAAAACCGCTTGTTCCAGCGTTTGGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCGGCCGGCCCAGCGTTTTATCTGCCAGCCACAGGGCTAg                                                                                                                                                                                      <  2:216772/247‑1 (MQ=255)                                                               ggCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGaaa                                                                                                                                                                           <  1:188967/251‑1 (MQ=255)                                                                              cAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAAc                                                                                                                                                            <  1:278315/251‑1 (MQ=255)                                                                               aGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACg                                                                                                                                                           >  1:17075/1‑251 (MQ=255)                                                                                gCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACgg                                                                                                                                                          <  2:309588/251‑1 (MQ=255)                                                                                     taCAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAAcc                                                                                                                                                     >  1:2733/1‑251 (MQ=255)                                                                                           aCGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCAc                                                                                                                                               <  2:279309/251‑1 (MQ=255)                                                                                             gATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGc                                                                                                                                             >  1:357383/1‑251 (MQ=255)                                                                                                            aaGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCg                                                                                                                              <  1:161924/251‑1 (MQ=255)                                                                                                                 aGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGcaccc                                                                                                                         >  2:356326/1‑250 (MQ=255)                                                                                                                    cATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGt                                                                                                                      >  1:36870/1‑251 (MQ=255)                                                                                                                          tGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTtcttct                                                                                                                >  1:122642/1‑251 (MQ=255)                                                                                                                                  aaGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGa                                                                                                          >  1:375766/1‑249 (MQ=255)                                                                                                                                      aagctcGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGcaca                                                                                                    <  1:361526/248‑1 (MQ=255)                                                                                                                                      aaTCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGcac                                                                                                     >  1:330750/1‑250 (MQ=255)                                                                                                                                             cAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGttt                                                                                             >  1:195181/1‑251 (MQ=255)                                                                                                                                                  gTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCATTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGGAACCCAGCACGCGGATTGCCGGGCCCGCAGCGCGTTCTTCTGCCGGAAGCACATCGGTTTTCCAc                                                                                        <  1:213722/251‑1 (MQ=255)                                                                                                                                                                       gCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTgg                                                                   >  2:21308/1‑251 (MQ=255)                                                                                                                                                                         gTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTggg                                                                  >  1:11760/1‑250 (MQ=255)                                                                                                                                                                             ggggCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCa                                                             <  2:105187/250‑1 (MQ=255)                                                                                                                                                                                 cacaGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGCCTATATCCTGGGAAGCAGAGc                                                         >  2:234483/1‑251 (MQ=255)                                                                                                                                                                                   caGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCa                                                        >  2:313/1‑250 (MQ=255)                                                                                                                                                                                           gtgtCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGAt                                               <  2:20369/251‑1 (MQ=255)                                                                                                                                                                                                                  caaCGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATcc                        <  1:212093/251‑1 (MQ=255)                                                                                                                                                                                                                   aaCGTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATcc                        >  1:234555/1‑250 (MQ=255)                                                                                                                                                                                                                      gTCGCGATAATACAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGCAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCCTCCAGCg                    >  2:285372/1‑251 (MQ=255)                                                                                                                                                                                                                              aataCAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGTTTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTggg            >  2:259474/1‑251 (MQ=255)                                                                                                                                                                                                                               ataCAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGt           >  1:298701/1‑251 (MQ=255)                                                                                                                                                                                                                                taCAGGGGATCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTg          >  1:18568/1‑251 (MQ=255)                                                                                                                                                                                                                                taCAGGGGATCAACAGTGTCGCAATTAACCAGGACATGCCATGACAGCACAGCCATTTATATGCCAGCAACCCGGCTAGCCCGCCGGAACACAGCATCCAAAAACGGTAACCAAGCACGATGATTGCCGCGACCGAACCACGTTCTTCTGCCGGACGCACATCGGTTTTCAACGCATCGAAGACTATATCCTGGGAAGCAGAGAAAAACGCGATCACCACTGCCAGAGCCGCCAACCAGCGGAGTTggttg          >  1:234203/1‑248 (MQ=255)                                                                                                                                                                                                                                        aTCAACAGTGTCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTc  >  2:129043/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ACGCCTACTTCACCCGCATCAAACCCGACGCCGCGAATCAAAAACGTGGTTGTCAGGCTCATGGCGAATGCGTCGCCCAGCTTATACAGCACGATAAGAAGCAAAATAAGCCAGGCATTATTGCGACCAAAGAAATCTCGCAGAGGTGCAACAACCGCTTGTTCCAGCGTTTTGGGCACAGGAATGGTGTCGGTTGGTTCTGGTGCAAGCAACGTCGCGATAATACAGGGGATCAACAGTGCCGCCATTAACCAGTACATGCCCTGCCAGCCCAGCCATTTATCTGCCAGCCACAGGGCTAGGCCGCCGGAAACCAGCATCCCTAAACGGTAACCCAGCACGCTGATTGCCGCGCCCGCACCACGTTCTTCTGCCGGAAGCACATCGGTTTTCCACGCATCGAAGACTATATCCTGGGAAGCAGAGCAAAAAGCGATCACCACTGCCAGCGCCGCCATCCAGCGGAGTTGGGTGCCTGGTTC  >  NC_000913/452761‑453242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑