Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	445,499	C→T	E390K (GAA→AAA)	yajR ←	putative transport protein YajR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,499	0	C	T	100.0%	55.8 / NA	17	E390K (GAA→AAA)	yajR	putative transport protein YajR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/6);  total (11/6)

GCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445262‑445690                                                                                                                                                                                                                                              |                                                                                                                                                                                                gCCTGATAAGCGTAGAGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTaatg                                                                                                                                                                                    >  1:285773/1‑248 (MQ=255)           cGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTCTGCCTGACGAATTGCCTGATCTCTCTAAAAGCAATACGCCCCTTTGCCGTCGCTTTTCCCATAAGCTGAATCATCTTCTTCTGCAATCAACACTTATTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCACTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGCTTTTTCATGGTCCTAGCGACTGTc                                                                                                                                                                           >  1:372172/1‑250 (MQ=255)                   agcagGCAATTCGCAGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACTCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGTACTTGCGACTGTccgaccaact                                                                                                                                                                  >  1:311247/1‑242 (MQ=255)                     cagGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGTACTGGCGCCTGTCAGCCCCACTGc                                                                                                                                                                >  1:76480/1‑251 (MQ=255)                      agGCAATTCGCCGCGTGTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCg                                                                                                                                                               <  2:15738/251‑1 (MQ=255)                        gCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGc                                                                                                                                                             >  1:18259/1‑251 (MQ=255)                                              ccTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCACTGCGCCAGCGAGAAAt                                                                                                                                       >  1:54354/1‑251 (MQ=255)                                                 gACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCCGCCTTGCGCCAGCGAGAAATAcc                                                                                                                                    >  1:282980/1‑251 (MQ=255)                                                   cGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAcccc                                                                                                                                  <  2:270886/251‑1 (MQ=255)                                                                                                      aaaTAAGCGGAATTTTCTTCTTCTGCAATAAACACTTCTTTGATGCCTTCATTTTCTAGCAAACGCCTTTTTAACGCCTCGTTTGGGGCAATGTTCGCGGGGATTTCAATGCGCAAACGGCTGACATGCGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAGAAACCCCCCCTGACCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                               <  2:36632/249‑1 (MQ=255)                                                                                                          aaGCGGAATTTTCTTCTTCTGCAATCACCCCTTCTTTGATGCCTTCAGTTTCTAGCAACCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCGGGGATTTCAATGCGCAAACTGCTGACATCCGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacg                                                                           <  1:273698/251‑1 (MQ=255)                                                                                                            gCTGAATGTTCTTCTTCTGCAATCAACACTTCTTGGATGCCTTCAGTTTCTAGCAACCGCATTTTTAACGCCTCGTTTGCGGCAATTTTCGCGGGGATTTCAATGCGAAAACGGCTGACATACGGCGTTTTTTTCATGGTACGGGCACCTGTCAGCCACACTGCGGCCAGCATGGCGCCAGCGAGAAATACCCCTTGACCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcc                                                                         <  2:144706/251‑1 (MQ=255)                                                                                                                aaTGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcca                                                                        >  1:66233/1‑248 (MQ=255)                                                                                                                        tcttctGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAATACCCCCCGCCCGTCAAACATGCCGTTAATCCAGACGCCCAGCGAACCGCCAAACGCCACCCCAAAAAActggcc                                                             >  2:119222/1‑250 (MQ=255)                                                                                                                                           cttTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCCCACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTTACCGTCAAACATGCCGCTAATCCAGCCGCCCAGCGGAACGCCCATCGCCCCGCCCAGAAActggcttgggtggtcaacaaccacc                                          >  2:63184/1‑232 (MQ=255)                                                                                                                                                                                cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGGGGAGGAAACAACCATCGCCGGACCTTTGTAACCTGCTGGCGACTCTTTACTg      >  1:304979/1‑250 (MQ=255)                                                                                                                                                                                     cGTTTGCGGCAGTGTTCCCCGGGATTTAAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCAGGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa  <  1:188201/249‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                GCCTGATAAGCGTAGCGCAGCAGGCAATTCGCCGCGTTTTTTATGCCTGACGAATTGCCTGTTCTATCTCAAAGCGATTCGTCACTTTGCTGTCGATTTTCACATAAGCTGAATGTTCTTCTTCTGCAATCAACACTTCTTTGATGCCTTCAGTTTCTAGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445262‑445690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑