Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,103	G→A	I43I (ATC→ATT)	qorA ←	putative quinone oxidoreductase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,103	0	G	A	100.0%	107.7 / NA	33	I43I (ATC→ATT)	qorA	putative quinone oxidoreductase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/23);  total (10/23)

CCTTTCAGGAAGGATGCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTT  >  NC_000913/4263858‑4264333                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         ccTTTCAGGAAGGATGCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATaaa                                                                                                                                                                                                                                     <  2:89252/251‑1 (MQ=255) ccTTTCAGGAAGGATGCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATaa                                                                                                                                                                                                                                      >  1:51642/1‑250 (MQ=255)    ttCAGGAAGGATGCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAAt                                                                                                                                                                                                                                   <  1:285874/250‑1 (MQ=255)                gCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATgg                                                                                                                                                                                                                       <  2:358659/250‑1 (MQ=255)                        gtGCTCAAAAGAAATGGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTGAtttt                                                                                                                                                                                                              <  2:47533/250‑1 (MQ=255)                                            caggcagAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTcc                                                                                                                                                                                          <  2:15165/251‑1 (MQ=255)                                                           cTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGGCGCTGCGGGCATAGGTGTCAATAAAATAGATGCCGGTGGCGTTTTTTTTGGCCTGGATTTCCTtcttctcctgacagggcggg                                                                                                                                                                           >  2:201112/1‑234 (MQ=255)                                                                 ccGCAATAATGGTATGCACAGAGCGGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGaa                                                                                                                                                                      <  2:253782/250‑1 (MQ=255)                                                                                  acaGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAc                                                                                                                                                   <  1:202145/251‑1 (MQ=255)                                                                                  acaGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGATTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGAACTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAAATCTAC‑GGCTTggaatac                                                                                                                                                   >  1:323999/1‑245 (MQ=255)                                                                                          gTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCgg                                                                                                                                            <  1:221606/249‑1 (MQ=255)                                                                                            aaGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCa                                                                                                                                        <  1:201878/251‑1 (MQ=255)                                                                                             aGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAAGCCGCTGCCGATATATGTGGCAATAAAAATGATGCCGATGGCTTTATTTTCGACCTGGATTTCCTTCTCCGCCGGATCGGCAGGGGGGAACTCTACCGGCT‑GAAGTACCTTCCGGGcac                                                                                                                                        >  2:330329/1‑249 (MQ=255)                                                                                                           aCTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAAATCTAC‑GGCTTGAAGGAC‑TTTCGGGCCACCCGGGTTGTGaaa                                                                                                                          >  1:98015/1‑250 (MQ=255)                                                                                                                                  cctgccgTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TGCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGtcc                                                                                                  <  1:356049/251‑1 (MQ=255)                                                                                                                                               gCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTAt                                                                                      <  1:336355/250‑1 (MQ=255)                                                                                                                                                    acacCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTGGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCa                                                                                 <  1:17630/250‑1 (MQ=255)                                                                                                                                                     caccacTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCGTGTGTCCTCCAGAACGTATCGTCAgg                                                                               <  2:312815/251‑1 (MQ=255)                                                                                                                                                     caccacTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCGCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAgg                                                                               <  2:156349/251‑1 (MQ=255)                                                                                                                                                                aCTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCat                                                                    <  1:136519/251‑1 (MQ=255)                                                                                                                                                                 cTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCCCCGTGCTTTTGGAATTTAATTTGTGTTGCCATGTGTCCCCCAGAACGTATCGTCAGGGGCTGCTTCatt                                                                   >  1:218749/1‑250 (MQ=255)                                                                                                                                                                        cACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATa                                                              <  2:103992/249‑1 (MQ=255)                                                                                                                                                                               ccTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGa                                                     <  1:120452/251‑1 (MQ=255)                                                                                                                                                                                ctgctgCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGAAGCGGCTATTTGTGTAAATAAAAATGAAGCCGCTGGCTTTATTTTCGAAATAGATTTTATTCTCCGCCGGATCGGCAGGAGCGTACCAAAC‑GGCTTTAAATACATTCCGG‑CCCCCGCGCTTGTGCAATTCAAATTGTGTTTCCCTGTGTCCTCCCGAACCTATCGTCCGGGGCTGCTTCATATGATAACGTTTcca                                                     >  1:56485/1‑248 (MQ=255)                                                                                                                                                                                      ctTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGGAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGGTGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGGGGGGACTCTAC‑GGCTTTGAGGAA‑TTCCCGGCCCCCCGGCTTGTGGAAATCAAATCGTGTTTCCCTGGGTTCTCCCGAACGTATTGTCCGGGGCTGCCTCCTTTGATTAAGTTTTGAccccttt                                              >  2:118965/1‑247 (MQ=255)                                                                                                                                                                                              ccTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTGCGACCCATTCTTTAtctc                                      <  1:118965/251‑1 (MQ=255)                                                                                                                                                                                                 aaTCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGTTAAAGTTGCGACCCATTCTTTATCTCGGt                                   <  2:174836/251‑1 (MQ=255)                                                                                                                                                                                                             aGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCa                        <  2:5288/250‑1 (MQ=255)                                                                                                                                                                                                                         gggTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGaa            >  1:28657/1‑250 (MQ=255)                                                                                                                                                                                                                           gTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATTGTCAGGGTCTGCTTCATATGATAAAGTGTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATa         <  2:183734/251‑1 (MQ=255)                                                                                                                                                                                                                           gTAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATa         <  1:39778/251‑1 (MQ=255)                                                                                                                                                                                                                            tAAAGGCCGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATGCTCCGCCGGAGCGGCAGGAGTTAACTCGAC‑GGCTTTAAGTAC‑TGCCGGGCCACCGTGCTTGTGAAATGCAATTCGTGTTGCCATGGGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCata                                                                   <  1:210127/192‑1 (MQ=255)                                                                                                                                                                                                                                  ccGCTGCGGATATATGTGTCAATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCtt  >  2:304725/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         CCTTTCAGGAAGGATGCCGCAGCTTGCTCAAAAGAAATTGCCGCAGGCAGAATCGCCGCTTTATCCGCAATAATGTTATGCACAGAGCTGTAAGCGCCTAACGCCGACTGCGCATAGACTACACGATCGCCTGCCTTAATATGCTTTACACCACTGCCGACTTTACTCACGATGCCTGCTGCTTCGGTGCCTAATCCGCTGGGTAGCGATGGCGGCGGGTAAAGGCCGCTGCGGATATATGTGTCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTAC‑GGCTTGAAGTAC‑TTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTT  >  NC_000913/4263858‑4264333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑