Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,115,770	C→T	A485T (GCA→ACA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,115,770	0	C	T	100.0%	155.4 / NA	45	A485T (GCA→ACA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

AGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCG  >  NC_000913/4115527‑4116008                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               agcGCCGCTGATTCGGTGACGCGGGAGAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACgg                                                                                                                                                                                                                                         <  1:375792/251‑1 (MQ=255)    gccgcTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTaa                                                                                                                                                                                                                                      <  1:124252/251‑1 (MQ=255)         tGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACg                                                                                                                                                                                                                                 >  2:195443/1‑251 (MQ=255)           aTTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCt                                                                                                                                                                                                                               >  2:316778/1‑251 (MQ=255)                  gACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGt                                                                                                                                                                                                                        <  1:211040/251‑1 (MQ=255)                            aaTTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCt                                                                                                                                                                                                              >  1:298806/1‑251 (MQ=255)                                cGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGAc                                                                                                                                                                                                          >  1:111105/1‑251 (MQ=255)                                      cAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACt                                                                                                                                                                                                    >  1:249751/1‑251 (MQ=255)                                              cGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACGCGCGCTCa                                                                                                                                                                                            <  1:82922/251‑1 (MQ=255)                                                      aGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCg                                                                                                                                                                                     <  1:146770/250‑1 (MQ=255)                                                      aGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGc                                                                                                                                                                                    <  2:106223/251‑1 (MQ=255)                                                         aaaCTCTAAGCAGTCAGGAACGGCGAGAAGCGCAGCCCAGGGGAAGGGAGGGCTTATTGATGCGTGCGGGGGTGCCACAGCCCGTTTCGCGACTACCGGACGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGAACGGAACTCGCGCTCAATCACCGCttt                                                                                                                                                                                 >  2:360916/1‑251 (MQ=255)                                                           aCTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTtct                                                                                                                                                                               >  1:378596/1‑251 (MQ=255)                                                           aCTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTtct                                                                                                                                                                               >  2:367120/1‑251 (MQ=255)                                                                 aGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCTCCGCTTTCTCTTGCa                                                                                                                                                                         <  1:322878/251‑1 (MQ=255)                                                                     gTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCtc                                                                                                                                                                     <  2:311410/251‑1 (MQ=255)                                                                                         tAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACt                                                                                                                                                                                                    >  2:303752/1‑200 (MQ=255)                                                                                         tAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACt                                                                                                                                                                                                    <  1:303752/200‑1 (MQ=255)                                                                                            cacaGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCaa                                                                                                                                              <  1:317382/251‑1 (MQ=255)                                                                                             acaGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAAc                                                                                                                                             >  2:352269/1‑251 (MQ=255)                                                                                             acaGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAAc                                                                                                                                             >  1:88312/1‑251 (MQ=255)                                                                                                 gggAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGcc                                                                                                                                         >  1:345326/1‑251 (MQ=255)                                                                                                     agggagggATTATTGATGTGTGTGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGc                                                                                                                                     >  1:170520/1‑251 (MQ=255)                                                                                                       ggagggaTTATGTATGTGTGCGGGGTTGCCCCCGCACGTTTCGGTACTACCGGATGCGGCATAAACTCTTCATTCGGCATTTACATTATTGGTCGTTTTCTTCCCACGCCTTCGCGCGTTTAACCGCTTTTGTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTGTCGATGCTTGGACGGAACTCGCGCTCAATCACCCCTTTCCCTTGCGGCTCGCCTAGTTTCTGCCAGAAGCCAGCCTCCAGGCCt                                                                                                                                   <  2:228797/251‑1 (MQ=255)                                                                                                                 ttGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCCCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAg                                                                                                                          <  2:298806/250‑1 (MQ=255)                                                                                                                     tgtgtgCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGc                                                                                                                     >  2:296236/1‑251 (MQ=255)                                                                                                                              ggTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGc                                                                                                            >  1:119708/1‑251 (MQ=255)                                                                                                                                  gCCCCCGCACGTTTGGGGACTCCCGGATGCGGCATAACCGCTTCATCCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCTTTTAACCGCTTTTTTCCAGCCTGTTTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGACCTCGCGCTCAATCCCCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGt                                                                                                         <  2:81404/250‑1 (MQ=255)                                                                                                                                  ccccccGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTg                                                                                                        <  2:119708/250‑1 (MQ=255)                                                                                                                                     cccGCACGTTTCGGGACTACCGGATGCGACATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACt                                                                                                     <  1:213715/251‑1 (MQ=255)                                                                                                                                                             tGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCAAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAAcg                                                                             >  2:89517/1‑251 (MQ=255)                                                                                                                                                                   aTAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTg                                                                       >  2:102685/1‑251 (MQ=255)                                                                                                                                                                     aaaCGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGcc                                                                     >  1:321834/1‑251 (MQ=255)                                                                                                                                                                                tCGGCATTTACATTATTCGTCGTGTTCTCCCCACCCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCg                                                           <  2:313544/250‑1 (MQ=255)                                                                                                                                                                                        tACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAAc                                                  >  1:340995/1‑251 (MQ=255)                                                                                                                                                                                          cATTATTCGTCGTGTTCTTCCCCCGCCTTCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTg                                                <  1:185663/251‑1 (MQ=255)                                                                                                                                                                                             tatgcgtcgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCa                                              <  1:358351/246‑1 (MQ=255)                                                                                                                                                                                                    cgtGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGaa                                       <  1:352269/250‑1 (MQ=255)                                                                                                                                                                                                                 cGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTg                         >  2:352327/1‑251 (MQ=255)                                                                                                                                                                                                                       cgcgcgTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGcca                    <  1:259752/250‑1 (MQ=255)                                                                                                                                                                                                                        gcgcgTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGccacc                  >  2:244736/1‑251 (MQ=255)                                                                                                                                                                                                                        gcgcgTTTAACCGCTTTTTTCAAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACGCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGccacc                  <  2:242169/251‑1 (MQ=255)                                                                                                                                                                                                                          gcgTTTAACCGCTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGCGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCAt                >  2:372321/1‑251 (MQ=255)                                                                                                                                                                                                                                     cTTTTTTCCAGCCTGTGTAACGGTAATTACGCTCCGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAgg     >  1:115012/1‑251 (MQ=255)                                                                                                                                                                                                                                         tttCCAGCCTGTGTAACGGTAATTACGCTCAGTGGTTGCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCg  <  1:346858/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               AGCGCCGCTGATTCGGTGACGCGGGAAAATTCGATGGCAAGTTCTCGTCTCATAGCAAACTCTAAGCAGTAAGGAATGGCGCGAAGTGTAGCACAGGGGAAGGGAGGGATTATTGATGTGTGCGGGGTTGCCCCCGCACGTTTCGGGACTACCGGATGCGGCATAAACGCTTCATTCGGCATTTACATTATTCGTCGTGTTCTTCCCACGCCATCGCGCGTTTAACCGCTTTTTTCCAGCCTGCGTAACGGTAATTACGCTCAGTGGTTTCGATGCCTGGACGGAACTCGCGCTCAATCACCGCTTTCTCTTGCAGCTCGTCGAGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCGCAGGGCG  >  NC_000913/4115527‑4116008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑