Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	432,667	C→T	S62N (AGT→AAT)	yajI ←	putative lipoprotein YajI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,667	0	C	T	100.0%	161.5 / NA	44	S62N (AGT→AAT)	yajI	putative lipoprotein YajI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/21);  total (23/21)

GGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCACTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAG  >  NC_000913/432424‑432889                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               ggggCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGa                                                                                                                                                                                                                         <  1:217698/251‑1 (MQ=255)  gggCATTAATCAATTGATTCTGCACATTGCTTTCCTGCACGATGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTAACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGAt                                                                                                                                                                                                                        >  1:135105/1‑251 (MQ=255)   ggCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGAtt                                                                                                                                                                                                                       >  1:53864/1‑251 (MQ=255)     cATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGAGGGCAAGGGCTCATTCGCCTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGAtttt                                                                                                                                                                                                                     >  1:252144/1‑251 (MQ=255)               ttGATGCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGGGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCAAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGcc                                                                                                                                                                                                           >  2:326982/1‑251 (MQ=255)                tGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCt                                                                                                                                                                                                          >  2:249070/1‑251 (MQ=255)                       gCACAGTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTg                                                                                                                                                                                                    <  2:29873/250‑1 (MQ=255)                            ttGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGt                                                                                                                                                                                              >  1:342864/1‑251 (MQ=255)                              gATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTg                                                                                                                                                                                             <  2:362214/250‑1 (MQ=255)                                 ttCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGAttt                                                                                                                                                                                         >  1:69505/1‑251 (MQ=255)                                       aaaGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTg                                                                                                                                                                                   <  1:38068/251‑1 (MQ=255)                                                     ttCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGt                                                                                                                                                                     >  1:7896/1‑251 (MQ=255)                                                                    cATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTgg                                                                                                                                                      <  1:361525/251‑1 (MQ=255)                                                                       atTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGtt                                                                                                                                                    <  1:91493/250‑1 (MQ=255)                                                                              aGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGc                                                                                                                                            >  2:233179/1‑251 (MQ=255)                                                                                ttCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTg                                                                                                                                          >  1:223762/1‑251 (MQ=255)                                                                                  ccGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGAc                                                                                                                                        >  1:140453/1‑251 (MQ=255)                                                                                       gAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCtg                                                                                                                                    <  1:105990/250‑1 (MQ=255)                                                                                       gAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCt                                                                                                                                     <  1:249070/249‑1 (MQ=255)                                                                                                                     cTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTtgctgc                                                                                                      >  2:298186/1‑250 (MQ=255)                                                                                                                           aCGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTTCACACAgg                                                                                               <  1:145565/251‑1 (MQ=255)                                                                                                                             gTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGgcg                                                                                             >  1:14647/1‑251 (MQ=255)                                                                                                                                gtgtAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCt                                                                                           >  2:61590/1‑250 (MQ=255)                                                                                                                                 tgtAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGAt                                                                                                                                                                                           >  1:303651/1‑153 (MQ=255)                                                                                                                                 tgtAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGAt                                                                                                                                                                                           <  2:303651/153‑1 (MQ=255)                                                                                                                                  gtAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTaa                                                                                         <  1:58938/250‑1 (MQ=255)                                                                                                                                     aGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCt                                                                                     >  1:97302/1‑251 (MQ=255)                                                                                                                                             gCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCGCTTTGCTGCACACAGGCGCTAAGGCTGAACAgg                                                                              <  1:158784/250‑1 (MQ=255)                                                                                                                                                aTCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTc                                                                          >  1:312222/1‑251 (MQ=255)                                                                                                                                                    gCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCAGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCcag                                                                      <  2:256259/251‑1 (MQ=255)                                                                                                                                                        cAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCcagcag                                                                   <  1:161403/250‑1 (MQ=255)                                                                                                                                                             gTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAg                                                             <  2:121919/251‑1 (MQ=255)                                                                                                                                                               aaTATTCACCAGCGACATACGTAAAGGACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAATGTAAACCCCATTGCGGGATTTTGCATTCAGCCTGTTTTGCTGAGTGAGTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCGGACGCTGGGTTTCATCTGTCGCACTTCGCTTTGCTGCACGCAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTc                                                           <  2:185101/251‑1 (MQ=255)                                                                                                                                                                   ttCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaa                                                        >  1:27603/1‑250 (MQ=255)                                                                                                                                                                   ttCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGaa                                                        >  1:143960/1‑250 (MQ=255)                                                                                                                                                                         aGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGtt                                                 <  2:278556/251‑1 (MQ=255)                                                                                                                                                                            gACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGt                                              >  1:301028/1‑251 (MQ=255)                                                                                                                                                                            gACATACGTAAAGTACCGATCTGGCATTCCAGTCTTGCCGGTGTTTTCGCTCCAGCCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGATGAACAGGCTTCCCAGCAGGAGCCGTCGAAAAACGTTAGt                                              >  1:301019/1‑251 (MQ=255)                                                                                                                                                                               aTACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTAtt                                           >  1:327236/1‑251 (MQ=255)                                                                                                                                                                                     aaaGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAgg                                     <  2:306829/251‑1 (MQ=255)                                                                                                                                                                                                  ggCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTTCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTccc                              <  1:207860/245‑1 (MQ=255)                                                                                                                                                                                                               tGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGTCGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGcc           >  2:259503/1‑251 (MQ=255)                                                                                                                                                                                                                    gtgtTTTCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATg      <  2:359677/251‑1 (MQ=255)                                                                                                                                                                                                                         ttCGCTCCAGGCAGAAGGTAAACCCCATTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTag  <  1:259503/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               GGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCTGAATTTGCCCATATTCAACAGTTCCGCTGAATGCGGGCAAGGGATCATTCGACTCACCCTGAATACGTAGTGTAAGGGTGGTGCCATCTGCATCAGGCGTAATATTCACCAGCGACATACGTAAAGTACCGATCTGGCTTTCCAGTCTTGCCGGTGTTTTCGCTCCAGGCAGAAGGTAAACCCCACTGCTGGATTTTGCATTCAGCCTGTTTTGCTGAGTGATTTTGACTGTTTCTTGATTGAGTTGCGTCATCTCCTGGTTCAGCGTGCTGACGCTGTGTTTCATCTGTCGCACTTCACTTTGCTGCACACAGGCGCTAAGGCTGAACAGGCTTCCCAGCAGGAGCAGTCGAAAAACGTTAGTATTCATAGGTCGTCCCTTTCTCGTCGCAATTAGCCTAATGGTAG  >  NC_000913/432424‑432889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑