Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,567,058	G→A	L326L (CTA→TTA)	glgA ←	glycogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,567,058	0	G	A	97.1%	118.8 / NA	36	L326L (CTA→TTA)	glgA	glycogen synthase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (12/22);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGCCCACCGGTGCGCCGCACTAACGGCAGCGTACCGTACTTCAATCCATAAAGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAGC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTAC  >  NC_000913/3566830‑3567304                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        catgCCCACCGGTGCGCCACACTCACGGCATCGTTCCGTACTTTAATCCATAAAGTTGCGTTAAGCCTCTCGGTTCAAAACGCCGGTGCACCAGAATGACGTACGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTTATAGCCAATCTTAACGCCCCCCTTACCGGGGTATTCCGTTGCCGCCGCAATTAAACCTTCCTGCAGCACCGGATCTCCCGCGCCAAGTAAC‑GCCAGATGCCCGGCCGGCTcc                                                                                                                                                                                                                                  <  2:316593/248‑1 (MQ=255)              gggccgcACTAACGGCAGCGTACCGTACTTCAATCGATAAAGGTGCGTTAAGCCGCACGGTTCAAAACGGCGGGGCACCAGAATGACGCCCGCGCCGCCCATAATGCAATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCGGACCGGTGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAGC‑GCCAGCTGCCCGCCCTGCTCAAGAAGACCCGGTa                                                                                                                                                                                                                     <  2:331005/249‑1 (MQ=255)                 ccgcACTAACGGCAGCGTACCGTACTACAATCCATAAAGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAgg                                                                                                                                                                                                                  <  1:43985/251‑1 (MQ=255)                                      gtacTTCAATCCATAAAGTTGCGTTAAGACGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGAAGCGAAAATGCTTCGTGATAACCAATCTGAACGCACACCTGACCGGGGTATTCCGATGACGCCGAAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTACC‑GACAGCTGCCCGCCCTGCTCAAGAAGACCCGGTAAGGCCTCCAGACCCAGGTCGAGAc                                                                                                                                                                                             >  2:374322/1‑251 (MQ=255)                                             aaTCCATAAAGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATGCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGAGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTctg                                                                                                                                                                                      <  1:369264/251‑1 (MQ=255)                                               tCCATAAAGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGACGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTctggc                                                                                                                                                                                    >  2:228262/1‑251 (MQ=255)                                                             ttaagatgCACGGTTCAAAACGGCTGGGCACCAGCATGACGTCCGCGACGCCCATAATGCGATGCGAAAATGCTACGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTc                                                                                                                                                                      >  2:246851/8‑251 (MQ=255)                                                                aGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTcacc                                                                                                                                                                   <  1:133894/251‑1 (MQ=255)                                                                            cAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGc                                                                                                                                                       <  2:40821/251‑1 (MQ=255)                                                                               aaCGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGc                                                                                                                                                    <  2:114066/251‑1 (MQ=255)                                                                                           ccAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCa                                                                                                                                        <  1:304091/251‑1 (MQ=255)                                                                                                aTGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCtt                                                                                                                                   <  2:253462/251‑1 (MQ=255)                                                                                                   aCGTCCGCGCCGCCCATAATGCGATGCGAAAATGTTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCGTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCTCCCTGCTCCAGAAGACCCTGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACAGGTCACCACTGTAAAAAGCGGCACTTTATCGTCAACCTTAAg                                                                                                                                <  1:165806/251‑1 (MQ=255)                                                                                                                 cATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGAttt                                                                                                                  <  1:72588/251‑1 (MQ=255)                                                                                                                         gatgcgaAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGAGCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTGTGGCTGGGCAGACGGCTCACCACTGCAAAAAGCGGCACTGTATCGGCAACCTTAAGCCCCATTGCGATTTGTAACTgg                                                                                                          <  1:236467/251‑1 (MQ=255)                                                                                                                               aaaaTGCTTCGTGATAGCCACTCAGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGCAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCtt                                                                                                     >  2:25526/1‑250 (MQ=255)                                                                                                                                           gATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTa                                                                                        <  2:103043/251‑1 (MQ=255)                                                                                                                                                  aaTCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGTCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCa                                                                                 <  1:17789/251‑1 (MQ=255)                                                                                                                                                      tGAACGACCACCTGACCGGGGTATTCCGCTGCCGCCGCACGGAAACCTTACTGCAGCACCGCATCGACCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCGGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCAACGTAAACATCGGCACTTTATCGTCCACCTTAAGCCCCATTGCGCTTCGTAACCGGAGCTTATTTTCCGCTTTATCTTCCAACgg                                                                             >  2:249728/1‑250 (MQ=255)                                                                                                                                                       gAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAAc                                                                               <  1:58687/248‑1 (MQ=255)                                                                                                                                                          cGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAAAAAACCTTGCTGGAGCACCGGATCGCCCGCCCCGA‑TAACTGCCAGCTGCCCACCCTGCTCCACAAGCACCGGTAAGGCTTCCAGCACCAGGCCGCGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCACAAAGCGGCCCTTTCTCGTCAACCTTAAGCCCCTTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATcg                                                                         >  1:27601/1‑251 (MQ=255)                                                                                                                                                                    aCCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAAc                                                               <  1:186276/251‑1 (MQ=255)                                                                                                                                                                          gTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATGTTCCGCTGTATCTTCCAACGTATCGCGGGTGTAACGCGAgg                                                         <  2:175620/251‑1 (MQ=255)                                                                                                                                                                                    gccgccGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTAGTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGt                                                 <  1:149120/249‑1 (MQ=255)                                                                                                                                                                                       gccgcAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                            <  2:291045/251‑1 (MQ=255)                                                                                                                                                                                          gcAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGGGTAACGCGAGGCCAACAGTAAGTCCGt                                         <  2:351068/251‑1 (MQ=255)                                                                                                                                                                                              ggAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTctct                                     <  2:116176/251‑1 (MQ=255)                                                                                                                                                                                                  aCCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGAc                                 >  1:157351/1‑251 (MQ=255)                                                                                                                                                                                                        cTGCAGCACCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTcc                              >  1:138691/1‑248 (MQ=255)                                                                                                                                                                                                          gcagcaCCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGAtt                         >  2:46984/1‑251 (MQ=255)                                                                                                                                                                                                               aCCGGATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTtctc                    >  1:333957/1‑251 (MQ=255)                                                                                                                                                                                                                  ggATCGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACAAGGTCGAGACCTTTCTGGCTGGTCCGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTc                 >  1:353477/1‑251 (MQ=255)                                                                                                                                                                                                                      cGCCCGCGCCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg             <  1:106556/251‑1 (MQ=255)                                                                                                                                                                                                                           gcgcCGAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGtt        >  2:270274/1‑251 (MQ=255)                                                                                                                                                                                                                                gAGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGt    >  2:24217/1‑250 (MQ=255)                                                                                                                                                                                                                                 aGTAAC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGTTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTAc  <  1:221576/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        CAAGCCCACCGGTGCGCCGCACTAACGGCAGCGTACCGTACTTCAATCCATAAAGTTGCGTTAAGCCGCACGGTTCAAAACGGCTGGGCACCAGAATGACGTCCGCGCCGCCCATAATGCGATGCGAAAATGCTTCGTGATAGCCAATCTGAACGCCCACCTGACCGGGGTATTCCGCTGCCGCCGCAAGGAAACCTTCCTGCAGCACCGGATCGCCCGCGCCGAGTAGC‑GCCAGCTGCCCGCCCTGCTCCAGAAGACCCGGTAAGGCTTCCAGCACCAGGTCGAGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTAC  >  NC_000913/3566830‑3567304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑