Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,549,730	G→A	T114I (ACC→ATC)	malQ ←	4‑alpha‑glucanotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,549,730	0	G	A	100.0%	115.3 / NA	35	T114I (ACC→ATC)	malQ	4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/20);  total (15/20)

CCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTT  >  NC_000913/3549494‑3549970                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  cgggagagagCGCATGAATCGGGTTCAGGCCAATGAACACCCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCgtg                                                                                                                                                                                                                                     <  2:181261/245‑1 (MQ=255)      aagagCGCATGAAGCGGGTTCAGCCCAATGACCGCCCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATa                                                                                                                                                                                                                                <  1:273262/250‑1 (MQ=255)                          cAGGCAAAGGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTtcgtcg                                                                                                                                                                                                            <  1:346078/251‑1 (MQ=255)                                   gAACGCCCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATACCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGGTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGa                                                                                                                                                                                                   <  2:343596/251‑1 (MQ=255)                                                 gTTTTGCCACATCCACCAGCAGCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCGGAACGCAGGCACCCCACAGCGTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCGCCCGGCAATGCGCGCGCTGGTCGGCCTGGATGAGTGTCAGCGGGTGATAACCTGTCGGCAGCGTCGTCGGTAGATTGAACGCTTTGcccccc                                                                                                                                                                                     <  2:365975/251‑1 (MQ=255)                                                     tGCCACATCCACCAGCATCGCTTTGAGATCGCCCCAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGACCGAAGCAACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAAAAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCCGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGtt                                                                                                                                                                                  >  1:168832/1‑250 (MQ=255)                                                              aaccaGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATACCCTACCGGCAGCTTCGTCGGTAGCTTGAACGATTTGCCCCCCGTTACATGGCCtt                                                                                                                                                                        >  2:199875/2‑251 (MQ=255)                                                                           ttGAGATCGCCAAAATCCCCAATTCCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTATATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGtt                                                                                                                                                             <  1:156818/249‑1 (MQ=255)                                                                           ttGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAAAGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTcc                                                                                                                                                           >  2:21354/1‑251 (MQ=255)                                                                                    ccAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggt                                                                                                                                                  <  1:193542/251‑1 (MQ=255)                                                                                     cAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtg                                                                                                                                                 <  1:342064/251‑1 (MQ=255)                                                                                      aaaaTCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCggtgg                                                                                                                                                <  1:295991/251‑1 (MQ=255)                                                                                                  aCCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGcagccagc                                                                                                                                    >  2:349220/1‑251 (MQ=255)                                                                                                                  gATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGTGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATGGAACGCTTGGCCCCCCGGTACATGGCCTTTGTGCTGCGTTCCTGCGTCGGTGGGCAGCAGCCATCTATATTCGCCGCTGcc                                                                                                                    <  2:180624/251‑1 (MQ=255)                                                                                                                     cGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑Ct                                                                                                                 <  2:174673/250‑1 (MQ=255)                                                                                                                        aGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTccac                                                                                                             <  2:39020/251‑1 (MQ=255)                                                                                                                        aGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTccac                                                                                                             >  2:97703/1‑251 (MQ=255)                                                                                                                           gTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTccaccac                                                                                                          <  1:168835/251‑1 (MQ=255)                                                                                                                             ataAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTccaccacc                                                                                                         <  1:243096/250‑1 (MQ=255)                                                                                                                                             gCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCCCCATCGGCATTTTTTTGc                                                                                         >  2:206688/1‑250 (MQ=255)                                                                                                                                             gCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGc                                                                                         <  1:206688/250‑1 (MQ=255)                                                                                                                                             ccACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTGCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGATTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCAGCGGCATTTTTTTGcc                                                                                        <  2:286436/250‑1 (MQ=255)                                                                                                                                                                        aaCGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGAc                                                             >  1:78459/1‑251 (MQ=255)                                                                                                                                                                               gCGGGTCGTAACAGCGTTTCGGGGCGACAATCAACCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTGCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGtt                                                      <  2:140807/251‑1 (MQ=255)                                                                                                                                                                                          cAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACttt                                           >  1:308151/1‑251 (MQ=255)                                                                                                                                                                                               tttCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTgg                                      >  1:192210/1‑251 (MQ=255)                                                                                                                                                                                                      gCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTAcc                               >  2:214812/1‑250 (MQ=255)                                                                                                                                                                                                        gACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGtt                             >  2:41608/1‑251 (MQ=255)                                                                                                                                                                                                           aaTCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGAt                          >  2:123352/1‑251 (MQ=255)                                                                                                                                                                                                           aaTCACCCGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGAt                          >  1:352521/1‑251 (MQ=255)                                                                                                                                                                                                              cccccGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTTTGATACCTTTCTGGCAGCTTCGTCGGTAGATTGAACTTTTTGCCCCCGTTTACAGGGCCTGTGTCGTGCTTTCCTTCTTCTGTGGGCAGCAGCCAGATATATTCGCC‑TTGCCGCTCCCCCCCCAGCGGCTTTTTTTTGCCTGTGGTATAAACCATGACAGTCGGGACGTGCTTTACCGCCACTTTCGTGGCGGTACGTTGATGCa                       <  1:25039/249‑1 (MQ=255)                                                                                                                                                                                                                  cGGCAATGCGCGCGCTGGTCGTCCTGGATGAGTGTCAGCGTTTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGCCATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATcgc                   <  1:97703/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaggcccgcgcTGGTCGTCCTGGATGAGTGTCAGCGGGTGATACCCTTCCGGCAGCTTCGTCGGTAGATTGACCGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTc                <  1:349220/244‑1 (MQ=255)                                                                                                                                                                                                                                   gtcgtcCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTTATGCATCGCGTCAAGCAACCGCCGtt  >  1:148028/1‑251 (MQ=255)                                                                                                                                                                                                                                   gtcgtcCTGGATGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTAATGTGATGAATCGCGTCTAGCAAAAGCCGtt  >  1:148019/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  CCGGATAGAGCGCATGAATCGGGTTCAGGCCAATGAACGACCCGCCACGTTTTGCCACATCCACCAGCATCGCTTTGAGATCGCCAAAATCCCCAATACCCCAGTTTTTTTCCGATCGCAGCGTATAAAGCTGAACGCAGGCACCCCACAGCTTTTGTTTATTCAGCAACGCCTGCGGTTCGTAACAGCGTTTCGGGGCGACAATCACCCGGCAATGCGCGCGCTGGTCGTCCTGGGTGAGTGTCAGCGTGTGATAACCTTCCGGCAGCTTCGTCGGTAGATTGAACGCTTTGCCCCCCGTTACATGGCCTTTGTACTGCGTTCCTTCTTCGGTGGTCAGCAGCCAGCTATATTCGCCGCTGCC‑CTCCACCACCATCGGCATTTTTTTGCCGCTGGTATAAACCATGACATTCGGGACTGGCGTTACCGCCACTTTCGTGGCGGTACGTTGATGCATCGCGTCAAGCAAACGCCGTT  >  NC_000913/3549494‑3549970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑