Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,502,726	G→A	E129E (GAG→GAA)	frlC →	fructoselysine 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,502,726	0	G	A	100.0%	144.4 / NA	43	E129E (GAG→GAA)	frlC	fructoselysine 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/14);  total (29/14)

GCATCAAACAAATCAAGGCGCTGGCGCAGACGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGACAGC  >  NC_000913/3502482‑3502972                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gcATCAAACAAATCAAGGCGCTGGCGCAGACGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAAcgt                                                                                                                                                                                                                                                   >  2:159823/1‑248 (MQ=255)                    gcTGGCGCAGACGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTAcg                                                                                                                                                                                                                               <  1:157246/251‑1 (MQ=255)                       ggCGCAGACGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTCTACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGTACTGTGTGAGTACGCgg                                                                                                                                                                                                                            >  2:132987/1‑251 (MQ=255)                               cGTATCAGATGCCGATTGTCGGCTATACGCCAGAAACCAACGGCTAGCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATc                                                                                                                                                                                                                    <  2:88363/251‑1 (MQ=255)                               cGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCACCGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGACAACATc                                                                                                                                                                                                                    >  1:43369/1‑251 (MQ=255)                                     aGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATg                                                                                                                                                                                                              >  1:95820/1‑251 (MQ=255)                                           cGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTCTAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCACAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTACTGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCt                                                                                                                                                                                                         >  1:210392/1‑250 (MQ=255)                                                    gCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGa                                                                                                                                                                                                >  1:77237/1‑250 (MQ=255)                                                       ataCGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCg                                                                                                                                                                                            >  1:184808/1‑251 (MQ=255)                                                                aaaCCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCg                                                                                                                                                                                   <  2:249688/251‑1 (MQ=255)                                                                                   taACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCgtgt                                                                                                                                                                >  2:110226/1‑251 (MQ=255)                                                                                   taACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCgtgt                                                                                                                                                                >  1:4203/1‑251 (MQ=255)                                                                                    aaCATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCgtgtg                                                                                                                                                               <  2:76622/251‑1 (MQ=255)                                                                                      cATGATGCTGGGCGATGAACATATCCGTCGCGAAAGCCTAGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGCTGACCGCGGGTTATACGCTGCTTTCCGCGGCCCACGCGGGCTCTCTCACGCCACCTAATGTTATCTGGGGACGGCTGGAAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCgtgtgt                                                                                                                                                              >  2:23020/1‑250 (MQ=255)                                                                                                 gCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAatgat                                                                                                                                                  >  1:347661/1‑251 (MQ=255)                                                                                                      gAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCt                                                                                                                                             <  1:174566/251‑1 (MQ=255)                                                                                                               cGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATgcgct                                                                                                                                    >  2:13217/1‑251 (MQ=255)                                                                                                               cGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATgcgct                                                                                                                                    >  2:208592/1‑251 (MQ=255)                                                                                                                     gAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATgcgctggcgct                                                                                                                              >  2:126552/1‑251 (MQ=255)                                                                                                                         gCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTg                                                                                                                          >  1:26683/1‑251 (MQ=255)                                                                                                                                  tGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTcgccg                                                                                                                 >  1:94592/1‑251 (MQ=255)                                                                                                                                      cAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGcc                                                                                                             >  2:304032/1‑251 (MQ=255)                                                                                                                                              cGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATc                                                                                                                                                                                                     >  2:144106/1‑155 (MQ=255)                                                                                                                                              cGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACAGCGGCATGGATCTAATc                                                                                                                                                                                                     <  1:144106/155‑1 (MQ=255)                                                                                                                                              cGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCAAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGc                                                                                                     >  2:262771/1‑251 (MQ=255)                                                                                                                                                aTGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGAGGGCTCTCTCACGCCACCTAGTGTTCTCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGCACCGTTAACGCCGTATGAATCGCACGTCGTGTGTACTGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCAt                                                                                                   >  2:188862/1‑251 (MQ=255)                                                                                                                                                                            gTTATACGCCGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTcc                                                                         >  1:306741/1‑249 (MQ=255)                                                                                                                                                                                  cGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGaa                                                                 <  1:81700/251‑1 (MQ=255)                                                                                                                                                                                     tGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCg                                                              >  2:37437/1‑251 (MQ=255)                                                                                                                                                                                      gATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCgg                                                             <  2:10043/251‑1 (MQ=255)                                                                                                                                                                                                  ccACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAACCATCGGCATGGAGCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGATTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCATGCGTAACCGGTGATGAGTTAtt                                                 <  2:336356/251‑1 (MQ=255)                                                                                                                                                                                                    aCGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTc                                               <  1:55903/251‑1 (MQ=255)                                                                                                                                                                                                                tcACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGc                                   >  1:18319/1‑251 (MQ=255)                                                                                                                                                                                                                       aCCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAAt                            <  1:141955/251‑1 (MQ=255)                                                                                                                                                                                                                       aCCTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAAAGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAAt                            >  1:162671/1‑251 (MQ=255)                                                                                                                                                                                                                         cTAATGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAAtt                           >  1:29431/1‑250 (MQ=255)                                                                                                                                                                                                                           aaTGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTa                          <  1:233796/249‑1 (MQ=255)                                                                                                                                                                                                                           aaTGTTATCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACg                        >  1:67380/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCTGGGGACGGCTGGCAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCt                  >  1:156233/1‑251 (MQ=255)                                                                                                                                                                                                                                               gtcAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGAc     >  2:42226/3‑250 (MQ=255)                                                                                                                                                                                                                                                gCAGAAAACCTAAGCAAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGACAg   <  1:262771/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGACAGc  >  1:192645/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cAGAAAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGACAGc  <  2:315055/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GCATCAAACAAATCAAGGCGCTGGCGCAGACGTATCAGATGCCGATTATCGGCTATACGCCAGAAACCAACGGCTATCCGTATAACATGATGCTGGGCGATGAACATATGCGTCGCGAAAGCCTCGACATGATCAAGCTGGCGATGGATATGGCAAAAGAGATGAACGCGGGTTATACGCTGATTTCCGCGGCCCACGCGGGCTATCTCACGCCACCTAATGTTATCTGGGGACGGCTGGCAGAGAACCTAAGCGAACTGTGTGAGTACGCGGAAAACATCGGCATGGATCTAATCCTCGAACCGTTAACGCCGTATGAATCGAACGTCGTGTGTAATGCTAATGATGTGCTTCATGCGCTGGCGCTGGTGCCTTCGCCGCGCCTGTTCAGCATGGTCGACATTTGCGCGCCGTATGTCCAGGCGGAACCGGTGATGAGTTATTTCGACAAACTGGGCGATAAATTACGTCATCTGCATATTGTCGACAGC  >  NC_000913/3502482‑3502972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑