Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,446,743	G→A	L48L (CTG→TTG)	rpsN ←	30S ribosomal subunit protein S14

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,446,743	0	G	A	100.0%	118.3 / NA	35	L48L (CTG→TTG)	rpsN	30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/19);  total (16/19)

GATACGGGTCAGCATATCCGCGATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATG  >  NC_000913/3446504‑3446974                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        gATACGGGTCAGCATATCCGCGATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGAGTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGCCGCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGATCTACCGCAGGAAACCATGCGGACGACCTGTTGGACGGCAGTGGTTACGATGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACCTGAGAACAg                                                                                                                                                                                                                              <  2:117352/251‑1 (MQ=255)           aGCATAGCCGCGAGCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGATACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTTAGAACAGCGTTCCAACg                                                                                                                                                                                                                    <  2:367268/251‑1 (MQ=255)                atacgcgATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGTACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGAAGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGACCAGCGTTCCAACGATCtt                                                                                                                                                                                                               >  2:105027/4‑251 (MQ=255)                atCCGCGATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCtt                                                                                                                                                                                                               >  2:89224/1‑251 (MQ=255)                    gcgATCGGATATTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGACTCACGCGGCAGAGTCTGCAACTTGAGACCAGCGTTCCAACGATCTtcgtc                                                                                                                                                                                                           >  2:173938/1‑251 (MQ=255)                      gATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCgg                                                                                                                                                                                                         >  1:240502/1‑251 (MQ=255)                         cGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGa                                                                                                                                                                                                        >  1:9328/1‑249 (MQ=255)                                tGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                                                                                                                  <  1:213539/248‑1 (MQ=255)                                                 ttACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGc                                                                                                                                                                                <  2:325674/249‑1 (MQ=255)                                                       ccGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTttcagtt                                                                                                                                                                        >  2:13310/1‑251 (MQ=255)                                                           gATTCAATTGGTGACAATTACCAAATAGCCTTTTTCAGAACCGGGATTTCACCGCGCATAGCGGCTTCACGGACATTAATACGGCTCAAACCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGCCGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAgc                                                                                                                                                                    >  2:238489/1‑251 (MQ=255)                                                                aaTTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGtt                                                                                                                                                               >  1:42359/1‑251 (MQ=255)                                                                       gACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGaa                                                                                                                                                        <  1:24066/251‑1 (MQ=255)                                                                       gACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGaa                                                                                                                                                        <  1:150871/251‑1 (MQ=255)                                                                                     aGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAgt                                                                                                                                            <  2:196582/249‑2 (MQ=255)                                                                                     aGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCt                                                                                                                                           <  2:151209/250‑1 (MQ=255)                                                                                                    cGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGtttt                                                                                                                            >  2:156209/1‑250 (MQ=255)                                                                                                          ttCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcg                                                                                                                      <  2:65566/250‑1 (MQ=255)                                                                                                                                 ggACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGcc                                                                                              >  1:183853/1‑251 (MQ=255)                                                                                                                                    ccTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCAtt                                                                                           <  1:207074/251‑1 (MQ=255)                                                                                                                                        aaTACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCTATTTAg                                                                                        <  2:291097/250‑1 (MQ=255)                                                                                                                                           aCGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAc                                                                                    <  2:48619/251‑1 (MQ=255)                                                                                                                                           aCGGCTCAACCAGACCTTCCGCACGACCACATGCCGACGCCCTGTTTGACGCCAGCGGTTACGCAGACGAGCCGGACTGCAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTAATTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAAc                                                                                    >  2:229250/1‑251 (MQ=255)                                                                                                                                              gCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCt                                                                                 >  1:206920/1‑251 (MQ=255)                                                                                                                                                               gCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACg                                                                >  2:148513/1‑251 (MQ=255)                                                                                                                                                                   gAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTtcgtcg                                                                                                                                                                                                          >  2:117254/1‑109 (MQ=255)                                                                                                                                                                   gAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGGTCCAACGATCTtcgtcg                                                                                                                                                                                                          <  1:117254/109‑1 (MQ=255)                                                                                                                                                                                        ttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                                                          <  2:273349/136‑1 (MQ=255)                                                                                                                                                                                        ttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                                                                                                                          >  1:273349/1‑136 (MQ=255)                                                                                                                                                                                         tGACGGCAGCGGTTACGCTGACGAGACGGACTGGAATCACGCGGCAGAGTCTGCAACTTGATAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTCCCTTACTTGCGGCGCGTCAAGTCAAACGCAGCCAGCAGAgc                                       <  2:77185/250‑1 (MQ=255)                                                                                                                                                                                                              cGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACTAGCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCGTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTTCGCGTTTTCCTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTTCCTTACTTGCGTAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTc                 <  1:206045/251‑1 (MQ=255)                                                                                                                                                                                                                  aCGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCag             <  1:259914/251‑1 (MQ=255)                                                                                                                                                                                                                   cGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCagt            >  2:55669/1‑251 (MQ=255)                                                                                                                                                                                                                            aTCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaat   <  1:219307/251‑1 (MQ=255)                                                                                                                                                                                                                              cACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGtggtaatg  <  1:68953/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                        GATACGGGTCAGCATATCCGCGATCGGATCTTGCATGCTCATCTGTCTTTACTCCCGTGATTCAATTGGTGACAATTACCAGCTAGCCTTTTTCAGACCCGGGATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGCTTAGCCATTTAGTAACCCTACCTTACTTGCGGAACGGGAAGTCAAAGGCAGCCAGCAGAGCGCGGCCTTCTTCGTCAGATTTCGCAGTAGTGGTAATG  >  NC_000913/3446504‑3446974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑