Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,943	C→G	G41G (GGG→GGC)	mlaF ←	intermembrane phospholipid transport system, ATP binding subunit MlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,943	0	C	G	100.0%	124.7 / NA	34	G41G (GGG→GGC)	mlaF	intermembrane phospholipid transport system, ATP binding subunit MlaF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/20);  total (14/20)

TGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTT  >  NC_000913/3339701‑3340178                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            tGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGAt                                                                                                                                                                                                                                     >  1:234839/1‑251 (MQ=255)                           aTGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACg                                                                                                                                                                                                            <  2:346131/250‑1 (MQ=255)                                   gcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTTAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGaa                                                                                                                                                                                                   <  1:346112/251‑1 (MQ=255)                                                 aCGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGa                                                                                                                                                                                         >  2:235379/1‑247 (MQ=255)                                                  cGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATg                                                                                                                                                                                    <  1:125304/251‑1 (MQ=255)                                                      gtaaaaTCCGTTCATATCAGTGAACAACGCCCCGGACTGAAAAAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCAAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCCCCCCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGc                                                                                                                                                                                <  2:34495/248‑1 (MQ=255)                                                             aCGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGcc                                                                                                                                                                         <  1:78866/251‑1 (MQ=255)                                                               gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCg                                                                                                                                                                                                                                   <  2:270916/191‑1 (MQ=255)                                                               gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCg                                                                                                                                                                                                                                   >  1:270916/1‑191 (MQ=255)                                                               gTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCAc                                                                                                                                                                       <  1:269987/251‑1 (MQ=255)                                                                    tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGt                                                                                                                                                                   >  1:33481/1‑250 (MQ=255)                                                                        cgTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGCGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAACTATTATCGAAGATGCAGCGATTGCCACGCGTAAAAc                                                                                                                                                              >  2:365557/2‑251 (MQ=255)                                                                          tGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTg                                                                                                                                                            >  1:229064/1‑251 (MQ=255)                                                                                                  aCATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTc                                                                                                                                    >  1:371324/1‑251 (MQ=255)                                                                                                            ccGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGAc                                                                                                                           <  1:6927/250‑1 (MQ=255)                                                                                                             cGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTg                                                                                                                         <  1:10096/251‑1 (MQ=255)                                                                                                                  gcgcACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTcc                                                                                                                     >  1:65411/1‑250 (MQ=255)                                                                                                                    aagttgattaCAGGGGCGAACGACATATCGCCGGAATATTCTCCCCGTCTAAAAGGTTCTCCCCA‑TGTTCGGGTTCGATTTTCCCGCCAATCAGACGGAGTATCGTCGTTTTACCGAGGCCCGATGGGCCCATGCGCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGGTTGCCACGCGTAAAACTGACATCACGCATATGGTCCAAATTCGCCACAGACTGCTCCATa                                                                                                                  <  2:322572/243‑1 (MQ=255)                                                                                                                           ataCAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCAcc                                                                                                           >  1:214217/1‑251 (MQ=255)                                                                                                                                     gAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTg                                                                                                 >  2:315721/1‑251 (MQ=255)                                                                                                                                      aacaagaCATCGCCGTAATTTCCTCACCATCAAAAAGGTTCTCACCA‑TGATCTGGTGCGTTTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGc                                                                                                <  2:279015/247‑1 (MQ=255)                                                                                                                                             cATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAtt                                                                                         <  1:338849/251‑1 (MQ=255)                                                                                                                                              aTCGCCGGAATATTCTCACCATCAAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGAttt                                                                                        <  1:77587/251‑1 (MQ=255)                                                                                                                                                    ggAATATTCTCACCATCGAACAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGCTGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTa                                                                                    >  2:75259/1‑249 (MQ=255)                                                                                                                                                       atatTCTCACCATCGAAAAGGATCTCCCCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCAt                                                                               <  1:250325/251‑1 (MQ=255)                                                                                                                                                             cccccATCGAAAAGTATCTCACCA‑TGATCTGGTTCGTTTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCt                                                                         <  2:176033/248‑1 (MQ=255)                                                                                                                                                                      aaaaGGATCTCACCAGTG‑TCTGGGGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCGTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAc                                                                <  2:55806/251‑1 (MQ=255)                                                                                                                                                                                           tGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTAc                                            >  1:34979/1‑251 (MQ=255)                                                                                                                                                                                            ggTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCGCCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACatat                                                <  1:176255/246‑1 (MQ=255)                                                                                                                                                                                                 gATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGa                                                           >  1:247106/1‑230 (MQ=255)                                                                                                                                                                                                 gATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGa                                                           <  2:247106/230‑1 (MQ=255)                                                                                                                                                                                                  aTTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGGCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAatat                                     <  1:343608/251‑1 (MQ=255)                                                                                                                                                                                                                                     cccGATGCCCGATGGGCCCATGACCGCCGTGATCTTCCCTCGCGGCACGGTCAGGAAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCCCCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTACCCTTGACCCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGttt  <  1:82772/250‑1 (MQ=255)                                                                                                                                                                                                                                     aCCGATGCCCGATGGGCCCATGATCGCCGTCATCTTCCCTCGCGGCACAGACACGGAAATATTATCGAAGCTGCAGAAATCGCCCCGCGAAAAACTGACATCGCGCATATCGACTACCATCCCCACAGCCTGCACCATAATTCACCCTTCCTCTTGCGTTGATTTTCTAAGCATGGCGCTCCATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGttt  >  1:172569/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCA‑TGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCTCGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACCCGCCGTGGTTAGCGAAAGCTGGCATTTGTTT  >  NC_000913/3339701‑3340178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑