Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	429,421	C→T	T592I (ACC→ATC)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	429,421	0	C	T	100.0%	158.0 / NA	43	T592I (ACC→ATC)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

ACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTT  >  NC_000913/429183‑429659                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               aCCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCgg                                                                                                                                                                                                                                    <  1:108369/251‑1 (MQ=255)          gTGGCGGTCGATGCGCACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCACAGGACGTACTGTTCAACAGGCAAGTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCTCGCTGATTAAAGTCGTCATCCTGTACGCAGTGGGTACCGGGGCTATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGCTGTTTATCGCGATTGTCGGTACGCGTGc                                                                                                                                                                                                                           <  1:54266/251‑1 (MQ=255)                  cGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTaa                                                                                                                                                                                                                   <  1:145311/251‑1 (MQ=255)                      gCGAACGTACTTATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACTCTGATTAAAGTCATCATCCTGTACGCAGTTGTTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGTCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAAcc                                                                                                                                                                                                                <  1:185342/250‑1 (MQ=255)                           cGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCtat                                                                                                                                                                                                          <  2:303519/251‑1 (MQ=255)                                 gATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATggcg                                                                                                                                                                                                    >  1:341747/1‑251 (MQ=255)                                      acgaacgTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCacg                                                                                                                                                                                               >  1:302588/1‑249 (MQ=255)                                      acgaacgTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAg                                                                                                                                                                                               <  2:221832/251‑1 (MQ=255)                                            gTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTc                                                                                                                                                                                         >  1:235857/1‑251 (MQ=255)                                                tAAAGCAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCa                                                                                                                                                                                        >  2:17249/1‑248 (MQ=255)                                                               cAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGTCGGCAAGCGCGTCGAGAAGCTGTCAATCTgag                                                                                                                                                                      <  1:306134/251‑1 (MQ=255)                                                                   ggACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGt                                                                                                                                                                  >  1:84755/1‑251 (MQ=255)                                                                    gACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTg                                                                                                                                                                 <  2:84755/251‑1 (MQ=255)                                                                     aCGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGATTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGc                                                                                                                                                                <  2:258656/251‑1 (MQ=255)                                                                               cAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGca                                                                                                                                                       <  1:5363/250‑1 (MQ=255)                                                                                 aCAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGccc                                                                                                                                                      >  1:100575/1‑247 (MQ=255)                                                                                  aaGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGCACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAgg                                                                                                                                                   <  2:361113/250‑1 (MQ=255)                                                                                          tgatgaAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATAc                                                                                                                                            <  1:307654/250‑1 (MQ=255)                                                                                                aGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGaa                                                                                                                                     <  2:374064/251‑1 (MQ=255)                                                                                                 ggTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGaac                                                                                                                                    >  1:58737/1‑251 (MQ=255)                                                                                                    tATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGaacaac                                                                                                                                 >  1:204121/1‑251 (MQ=255)                                                                                                         tGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAAc                                                                                                                            >  1:369076/1‑251 (MQ=255)                                                                                                                                             catgCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGac                                                                                        <  2:295326/248‑1 (MQ=255)                                                                                                                                              acGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGact                                                                                       >  1:337575/1‑251 (MQ=255)                                                                                                                                                  tGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACt                                                                                                      <  1:122028/232‑1 (MQ=255)                                                                                                                                                  tGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACt                                                                                                      >  2:122028/1‑232 (MQ=255)                                                                                                                                                               catcCTGTACGCAGTGGGTACCGTGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCAt                                                                       <  1:100325/250‑1 (MQ=255)                                                                                                                                                                     gTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCACGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGAATTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGt                                                                >  2:186191/1‑251 (MQ=255)                                                                                                                                                                        cGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGaacaac                                                                                                                                 >  1:153564/1‑183 (MQ=255)                                                                                                                                                                        cGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGaacaac                                                                                                                                 <  2:153564/183‑1 (MQ=255)                                                                                                                                                                              gggTACCGGGGCAATTAAAGGGTGCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCTCGATTGTCGGTACGCGGGCCATCGTAATCCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACGTTTGAGCAACTAATCCACGGCCGTAAAGTCTATGCCTTTATGCGCTGGGACTACTGGGCGTTCGGGATCTCTGGTCTGCTGTTa                                                       <  2:234183/251‑1 (MQ=255)                                                                                                                                                                                     ggggCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGca                                                                                                                                                       <  2:177248/148‑1 (MQ=255)                                                                                                                                                                                     ggggCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGca                                                                                                                                                       >  1:177248/1‑148 (MQ=255)                                                                                                                                                                                          aaTTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATc                                           <  1:261224/251‑1 (MQ=255)                                                                                                                                                                                             tAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGt                                         <  1:72233/250‑1 (MQ=255)                                                                                                                                                                                                        cgATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTCTAGCGATTGTCGGTACGCCTGCCATCGTAAAAACGCTATATGGCGGCCAGCGCGTCACGAAGCTGACAATCTGAGGAGAGCGATGAGGCACAGGAATATACTGTTGAACAACTAAACCAAGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTACTGTTCATCGCTGCTATCGATCTTATgggagt                             >  1:364190/1‑248 (MQ=255)                                                                                                                                                                                                              ctacCGGTATCGGTGTGGCGCCGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCgg                       >  2:78698/1‑251 (MQ=255)                                                                                                                                                                                                                        cGGTGTGGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGATGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTgg             <  2:266673/251‑1 (MQ=255)                                                                                                                                                                                                                         ggTGTGGAGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTAAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTAGTATGGGCGTGCGCGGCTTTAACTggg            >  2:311369/1‑251 (MQ=255)                                                                                                                                                                                                                            gtgGCGACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTgggtg          <  1:17249/250‑3 (MQ=255)                                                                                                                                                                                                                            gtgGCGACGTCGATGTTTATCGCGATTGCCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGAATTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTCTCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGc         >  2:325687/1‑251 (MQ=255)                                                                                                                                                                                                                                 gACGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGAt    >  2:299712/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGTCGATGTTTATCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGAttt  <  1:271748/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               ACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCGTGGCGCATTCAGTTCTATCTTCGATGCGAACATCACCACGCTGATTAAAGTCATCATCCTGTACGCAGTGGGTACCGGGGCAATTAAAGGGTTCGCGATTACTACCGGTATCGGTGTGGCGACGTCGATGTTTACCGCGATTGTCGGTACGCGTGCCATCGTAAACCTGCTATATGGCGGCAAGCGCGTCAAGAAGCTGTCAATCTGAGGAGTGCGATGTGGCACAGGAATATACTGTTGAACAACTAAACCACGGCCGTAAAGTCTATGACTTTATGCGCTGGGACTACTGGGCTTTCGGCATCTCTGGTCTGCTGTTAATCGCTGCTATCGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTT  >  NC_000913/429183‑429659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑