Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,215,073	T→C	Q134R (CAG→CGG)	mug ←	stationary phase mismatch/uracil DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,215,073	0	T	C	100.0%	166.1 / NA	46	Q134R (CAG→CGG)	mug	stationary phase mismatch/uracil DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

GCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCTGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGC  >  NC_000913/3214840‑3215318                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      gCGTCACCCGAGCCGTTCTGAAGTGCTGCGCAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACACGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTg                                                                                                                                                                                                                                      >  1:166174/1‑251 (MQ=255)  cGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTg                                                                                                                                                                                                                                      >  2:87436/1‑250 (MQ=255)           aGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCgtttgtt                                                                                                                                                                                                                            <  1:89380/251‑1 (MQ=255)            gCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCgtttgttt                                                                                                                                                                                                                           >  1:79479/1‑251 (MQ=255)               gTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTccc                                                                                                                                                                                                                        <  1:125967/251‑1 (MQ=255)                         gctgcGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCa                                                                                                                                                                                                              <  1:327408/251‑1 (MQ=255)                                         gACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATc                                                                                                                                                                                              >  1:25977/1‑251 (MQ=255)                                             aTTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTg                                                                                                                                                                                          <  2:245776/251‑1 (MQ=255)                                             aTTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTg                                                                                                                                                                                          <  1:304308/251‑1 (MQ=255)                                                          ccGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCAACAACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGt                                                                                                                                                                             >  1:13216/1‑251 (MQ=255)                                                            ggATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCGTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGttt                                                                                                                                                                           <  2:27481/251‑1 (MQ=255)                                                                cAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGccc                                                                                                                                                                       >  1:186494/1‑251 (MQ=255)                                                                   gCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGa                                                                                                                                                                    <  2:377437/251‑1 (MQ=255)                                                                        aCGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCCGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGc                                                                                                                                                               >  1:366161/1‑251 (MQ=255)                                                                        aCGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGc                                                                                                                                                               <  1:197495/251‑1 (MQ=255)                                                                                 ccGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTCTCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTGCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCGCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACGGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTg                                                                                                                                                      <  1:154501/251‑1 (MQ=255)                                                                                    gCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCgg                                                                                                                                                   <  1:118917/251‑1 (MQ=255)                                                                                      aCTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGcc                                                                                                                                                 >  2:28832/1‑250 (MQ=255)                                                                                      aCTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCt                                                                                                                                                 >  1:3164/1‑251 (MQ=255)                                                                                         aGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTg                                                                                                                                                <  1:256444/249‑1 (MQ=255)                                                                                         aGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTg                                                                                                                                                <  1:120463/249‑1 (MQ=255)                                                                                                ctGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTc                                                                                                                                       >  2:161596/1‑251 (MQ=255)                                                                                                             aCCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATc                                                                                                                          >  2:288098/1‑251 (MQ=255)                                                                                                                    cGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTAc                                                                                                                   >  1:375226/1‑251 (MQ=255)                                                                                                                             tttttATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTg                                                                                                          >  2:127398/1‑251 (MQ=255)                                                                                                                                                                      gATACGCTTCAACCAGTGTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGtt                                                                 <  2:77875/251‑1 (MQ=255)                                                                                                                                                                                  ccAGTTTCTCCAGTGAAACGCGACTTAACCCGCTGGGATTTGGCAGCACCCAAATCCGCGTCTAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCCCCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCCACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGtgcaag                                                     <  2:97422/251‑6 (MQ=255)                                                                                                                                                                                        tctcCAGTGAACCGCGACTTAAACCGCTGGGCTTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAACCTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTg                                               >  2:30772/1‑251 (MQ=255)                                                                                                                                                                                         ctcCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTgg                                              >  2:128976/1‑251 (MQ=255)                                                                                                                                                                                              gTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACg                                         >  1:76953/1‑251 (MQ=255)                                                                                                                                                                                                 aaaCGCGACTTAAACCGCTGCGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGCGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGcc                                       >  1:133414/1‑250 (MQ=255)                                                                                                                                                                                                  aaCGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCcac                                     <  2:278000/251‑1 (MQ=255)                                                                                                                                                                                                      cgACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACg                                 <  1:161596/251‑1 (MQ=255)                                                                                                                                                                                                       gACTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGACACAACg                                 >  1:12495/1‑250 (MQ=255)                                                                                                                                                                                                        aCTTAAACCGCTGGGATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGAt                               <  1:224712/251‑1 (MQ=255)                                                                                                                                                                                                           tAAACCGCTGGGATTTGGCAGCACCCAAATCCGAGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATaa                             <  2:80160/250‑1 (MQ=255)                                                                                                                                                                                                                    gggATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCcagcag                     <  2:179029/249‑1 (MQ=255)                                                                                                                                                                                                                      gATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGc                 <  1:306499/251‑1 (MQ=255)                                                                                                                                                                                                                      gATTTGGCAGCACCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGc                 <  2:79479/251‑1 (MQ=255)                                                                                                                                                                                                                            gcagcaCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGcc           <  1:197041/251‑1 (MQ=255)                                                                                                                                                                                                                            gcagcaCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGcc           >  1:3694/1‑251 (MQ=255)                                                                                                                                                                                                                             cagcaCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGcct          <  1:128976/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg      <  2:103011/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg      <  2:208967/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCCAAATCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTg      >  1:124765/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaTCCGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGc  >  1:70550/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      GCGTCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTGGACGATTAATCGGTAGGCCGGATCAGGCGTTACGCCGCACCCGGCACTAGGCCCTCTGCACAAACGCCACCTTTTCGGTGGCGTTTTTTATCGCCCACGCACTACCAGCGCCTGGTCCAGCTCGCGATACGCTTCAACCAGTTTCTCCAGTGAAACGCGACTTAAACCGCTGGGATTTGGCAGCACCCAAATCTGCGTCGAACCAATGGTGAGCGTTTGTTTCCCCCACTGTGCACCGCGCTGGCTGAATCCCTGTTCATATGCTTGTTTGCCCAGAATCGCCAACGCCTGCGGCTGATAATCTTCAATTTTTTCAATCAGCTTACGCCCGCCTGCGTGTAGCTCCTGCTTTGAAACTTCATTGGCTTGCACCGTTGGACGGTCTACCAGTTTGGTGACGCCACAACGATAATCCAGCAGATGCTGTGCCTCCTGCGGC  >  NC_000913/3214840‑3215318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑