Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,282	C→T	V191M (GTG→ATG)	trmB ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,282	0	C	T	100.0%	108.6 / NA	32	V191M (GTG→ATG)	trmB	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

ACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCT  >  NC_000913/3102038‑3102523                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  aCCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACTGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATGCTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAg                                                                                                                                                                                                                                             <  2:258904/251‑1 (MQ=255)       cGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATg                                                                                                                                                                                                                                       <  1:232528/251‑1 (MQ=255)                         cGAAAATCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTccc                                                                                                                                                                                                                      >  1:265766/1‑250 (MQ=255)                              aTCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAgtcgg                                                                                                                                                                                                                >  1:17951/1‑251 (MQ=255)                                ccTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAgtcggtc                                                                                                                                                                                                              <  1:296142/251‑1 (MQ=255)                                                cgtcgATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGc                                                                                                                                                                                              >  2:238757/1‑251 (MQ=255)                                                                              ggCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGtt                                                                                                                                                                >  1:322082/1‑251 (MQ=255)                                                                               gCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCAGACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTc                                                                                                                                                               <  2:234171/251‑1 (MQ=255)                                                                                             ccATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCt                                                                                                                                                 >  2:224762/1‑251 (MQ=255)                                                                                                 tatgacACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                                                                              <  2:90207/245‑1 (MQ=255)                                                                                                                      aaGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGgcg                                                                                                                        <  2:280077/251‑1 (MQ=255)                                                                                                                              tACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTtgt                                                                                                                >  1:305941/1‑251 (MQ=255)                                                                                                                                      gACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                                                                                        >  1:320558/1‑251 (MQ=255)                                                                                                                                      gACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGCTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                                                                                        >  2:262005/1‑251 (MQ=255)                                                                                                                                               gATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTCCGTAATCATTGCTCTCTGACAGGTTTTTAAAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaa                                                                                                >  1:173145/1‑250 (MQ=255)                                                                                                                                                           gttCAAATTTCGTCACCGGACGTGATTCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCATCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAcc                                                                                   <  1:37260/251‑1 (MQ=255)                                                                                                                                                              cgaaTTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCAGTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACAGCATTTCAAGCATAGGTTCCGCAGAAGGTTCCCTGTCGGTCGCCATATGTAATACGCCCCCCAGCTGCAGTTTGCTTGTTACCATTTCGGCAAACGGCACCTGAACGATACGGCGTTTAGTATTGGGCGCTTTGTTCCACGGGTCAGGGAAAAAGAGCTGCACCATg                                                                                <  1:252895/249‑1 (MQ=255)                                                                                                                                                              cAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCAt                                                                                 <  1:131339/250‑1 (MQ=255)                                                                                                                                                                          ccGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTg                                                                    <  1:238757/251‑1 (MQ=255)                                                                                                                                                                            ggACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTCTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTc                                                                  <  2:202610/251‑1 (MQ=255)                                                                                                                                                                             gACGTGATGCCGGACTCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCa                                                                 <  1:83284/251‑1 (MQ=255)                                                                                                                                                                               cGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAgg                                                               <  2:322082/251‑1 (MQ=255)                                                                                                                                                                                  gATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATTAATTGTCAGGaa                                                             <  1:263619/250‑1 (MQ=255)                                                                                                                                                                                       cGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCAttt                                                       >  1:133549/1‑251 (MQ=255)                                                                                                                                                                                        ggACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTTCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCAtttt                                                      <  1:24385/251‑1 (MQ=255)                                                                                                                                                                                        ggACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCAtttt                                                      >  1:9812/1‑251 (MQ=255)                                                                                                                                                                                               gTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATgcagc                                               >  1:14605/1‑251 (MQ=255)                                                                                                                                                                                                   gTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACtt                                           <  2:320558/251‑1 (MQ=255)                                                                                                                                                                                                         aTTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCg                                     <  2:347992/251‑1 (MQ=255)                                                                                                                                                                                                                 ctGACAGGTTTTTATAACCGTCAATAGAAGACATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCGAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGt                               <  2:265766/249‑1 (MQ=255)                                                                                                                                                                                                                                     tCAATAGAAGACATCATTTCACGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCt         >  2:359381/1‑251 (MQ=255)                                                                                                                                                                                                                                            aagaCATCATTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCAAGCGCAGGTTGCTTAAACct  >  1:266264/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATGTGCATTTTTTTACGCAGACGACGGCTACGGTTCTTTGCCATTATTTCACCCTCTCGAACATTAAGTCCCATACTCCGTGACCAAGACGATGACCACGTTGTTCAAATTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAATCATTTTATGCAGCACTTCAACCGCATCGTGACACATCACGCGCAGGTTGCTTAAACCT  >  NC_000913/3102038‑3102523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑