Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,457,433	C→T	G576D (GGC→GAC)	fadJ ←	3‑hydroxyacyl‑CoA dehydrogenase FadJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,457,433	0	C	T	100.0%	95.4 / NA	29	G576D (GGC→GAC)	fadJ	3‑hydroxyacyl‑CoA dehydrogenase FadJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/11);  total (18/11)

ACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGC  >  NC_000913/2457198‑2457662                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      aCCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGATACGGCTAACCTGCTCATCAACACACCGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATcgtcgt                                                                                                                                                                                                                        >  1:228594/1‑251 (MQ=255)       gCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCaaaa                                                                                                                                                                                                                  <  1:285180/251‑1 (MQ=255)            aTACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTga                                                                                                                                                                                                              >  2:251955/1‑250 (MQ=255)                cgcgCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTgaagaa                                                                                                                                                                                                          >  2:162784/1‑250 (MQ=255)                      aaTATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACAt                                                                                                                                                                                                   >  2:331267/1‑251 (MQ=255)                            cccGTAACGCACGCTACGGATAACCTGCTCCTCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGAGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCCTTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGc                                                                                                                                                                                               >  2:295021/1‑249 (MQ=255)                                                         catcaACACAACGTACTGCTTCATTCAGCATCAACATCACACACAGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCt                                                                                                                                                                >  1:357189/1‑251 (MQ=255)                                                               cacaACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGt                                                                                                                                                          <  2:380161/251‑1 (MQ=255)                                                                                    gCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCg                                                                                                                                     <  1:11457/251‑1 (MQ=255)                                                                                     cATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCgg                                                                                                                                    >  2:119034/1‑251 (MQ=255)                                                                                            aTCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGAt                                                                                                                             <  2:178176/251‑1 (MQ=255)                                                                                                      gTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTg                                                                                                                                                                                                <  1:285301/174‑1 (MQ=255)                                                                                                      gTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTg                                                                                                                                                                                                >  2:285301/1‑174 (MQ=255)                                                                                                                cTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGtt                                                                                                         >  2:56907/1‑251 (MQ=255)                                                                                                                                 gCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGTAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCCACCTCATCCAAAAGTTGGGTTGGGCCTACCgg                                                                                         >  2:83514/1‑250 (MQ=255)                                                                                                                                       gCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCACAAGTTGGATTGGGCCTACCGGAAAAcc                                                                                   >  1:163634/1‑250 (MQ=255)                                                                                                                                                       cAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCgg                                                                  <  2:366876/251‑1 (MQ=255)                                                                                                                                                           gggTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATc                                                              >  2:63702/1‑251 (MQ=255)                                                                                                                                                                 aTGGCGGGATCGACCTGTGTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGTCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAAtgtg                                                        <  2:31981/251‑1 (MQ=255)                                                                                                                                                                   ggCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGct                                                      >  2:86889/1‑251 (MQ=255)                                                                                                                                                                            gACCTGTTTTTTGCTTTTACGCCATTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGtt                                             >  2:26713/1‑251 (MQ=255)                                                                                                                                                                              ccTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGACGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGCCATCAATGTGCTCTACCCGTTCa                                           <  2:153293/251‑1 (MQ=255)                                                                                                                                                                                     tttGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTggg                                    >  2:319131/1‑251 (MQ=255)                                                                                                                                                                                             tACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATg                            >  1:257837/1‑251 (MQ=255)                                                                                                                                                                                              aCGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATg                            <  2:292722/250‑1 (MQ=255)                                                                                                                                                                                               cGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCg                          <  1:318841/251‑1 (MQ=255)                                                                                                                                                                                                       atGACCATAACGATAGAACCCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCACGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATATTTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTCCTCTACCCGTTCACCTTGGGTCACCATGCGGATAGCtt                  >  2:257191/2‑251 (MQ=255)                                                                                                                                                                                                        tGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTc                 >  1:278228/1‑251 (MQ=255)                                                                                                                                                                                                                       aaaCCCCGGCCATTTTTTCTGTCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGGCCCGGTGTCGATTCCGACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATGTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGgcgc  <  2:192640/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      ACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCATCAACATCACACACCGTTCAGCAACCTGCGGTGCGGAGATTCGCCCCTGCCCTTGTGTGCCAATCAGCGGGTAAATGGCGGGATCGACCTGTTTTTTGCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTCTGCCTTTGCGATCGTCGTTCAAAATTGAAGAAACAACATTTGCAGGCGCGCTAAAACGTTCTCCATAAGCGGCTTCCAGTACAGGAATAATTTTAGTCCCGGTGTCGATTCCTACCTCATCCAAAAGTTGGATTGGGCCTACCGGAAAACCAAATTTCACTAGCGCGGCATCAATGTGCTCTACCCGTTCACCTTGGGTCAACATGCGGATAGCTTCATTAATGTAAGGCGC  >  NC_000913/2457198‑2457662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑