Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,454,572	C→T	intergenic (‑2236/+511)	yfcS ← / ← yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,454,572	0	C	T	100.0%	83.6 / NA	24	intergenic (‑2236/+511)	yfcS/yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAATTGTATTT  >  NC_000913/2454329‑2454815                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    catTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCg                                                                                                                                                                                                                                              <  2:19993/251‑1 (MQ=255)         cgcgTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAGTGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCt                                                                                                                                                                                                                                      >  2:140137/1‑251 (MQ=255)            gTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAACTGATTAATGCGGACTGGCTTa                                                                                                                                                                                                                                    >  2:283363/1‑250 (MQ=255)              aTGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAAt                                                                                                                                                                                                                                 >  1:221001/1‑251 (MQ=255)                                ccAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGc                                                                                                                                                                                                               >  1:260412/1‑251 (MQ=255)                                 cAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGcc                                                                                                                                                                                                              >  1:171126/1‑251 (MQ=255)                                           gcATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTg                                                                                                                                                                                                     >  2:31005/1‑250 (MQ=255)                                               aCGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGCAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCgg                                                                                                                                                                                                >  2:103146/1‑251 (MQ=255)                                                                aagccccTACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAAGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTAcc                                                                                                                                                                                <  2:260717/247‑1 (MQ=255)                                                                       gACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATTGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGc                                                                                                                                                                        <  2:296076/251‑1 (MQ=255)                                                                             cccGTTGCCGCTGATCTCGTGACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCAGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTGCCATCGTGGCTCCAtt                                                                                                                                                                  <  2:260412/251‑1 (MQ=255)                                                                                              cGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCg                                                                                                                                                      <  1:150359/246‑1 (MQ=255)                                                                                                                                            gATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAg                                                                                                   <  2:272328/251‑1 (MQ=255)                                                                                                                                                                          gCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCa                                                                      <  1:31005/250‑1 (MQ=255)                                                                                                                                                                                                     ccAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCg                                          <  2:96440/251‑1 (MQ=255)                                                                                                                                                                                                      cAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCATATTTTTCGCAACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGc                                         <  1:189554/251‑1 (MQ=255)                                                                                                                                                                                                            ggCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATgg                                   <  2:358905/251‑1 (MQ=255)                                                                                                                                                                                                                aGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGt                               >  1:121895/1‑251 (MQ=255)                                                                                                                                                                                                                         cGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAg                       <  2:273115/250‑1 (MQ=255)                                                                                                                                                                                                                         cGAGGTAAGCCTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCAGACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAg                       <  1:229963/250‑1 (MQ=255)                                                                                                                                                                                                                                   cTGCGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTCAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGt             >  2:301969/1‑250 (MQ=255)                                                                                                                                                                                                                                      cGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCATCCTTAATTTCCTCGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAATCTGCGCAACCAGTCCCGGTGTCATACAATCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCATTAAATATCTTACTCTTCCGCCTATGGCTGTTTATTTAGTTGAACCTGTAAAt         <  1:373290/251‑1 (MQ=255)                                                                                                                                                                                                                                      cGGTAGTGAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAAt         >  2:347887/1‑251 (MQ=255)                                                                                                                                                                                                                                             gAAATGATTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAATTGTAttt  >  1:305759/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CATTACTGCGCGTATGTTGATAGTTGGTCTGCCAGTCAGCGCGCATACGCCATGGCCCCAGGTTAACCCCGACCGTCCCGTTGCCGCTGATCTCGTTACTGTCATCGCCGCCATTTTCTTCGTGTCGTGTTTGCGCAGTGATGCTGTAGTCCGCAATGATCCCGGAGATGCCATCATCCCAACGAGAAGGTGGATCCCAGTCGGGCCAGGTATATTCGAGGTAAGCCTGCGGTAGTGAAATGACTAATGCGGACTGGCTTAAATCAGCCTTAATTTCCACGCCTTCCAGTTGACCGGGCTTCAGGCATTTACCATCGTGGCTCCATTGCAGATTTTTCGCCACGTCTTCTTTTAAACCAAACTGCGCCACCAGTTCCGGTGTCAGACAAGCATAGCTTTTGCTAACGTCATCTTCACCAGCATACCAGTAAATATCGTACTCTTCCGCCAATGGCTGTTTATTTAGTTGAACCTGTAAATTGTATTT  >  NC_000913/2454329‑2454815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑