Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,452,924	C→T	intergenic (‑588/+2159)	yfcS ← / ← yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,452,924	0	C	T	100.0%	152.4 / NA	46	intergenic (‑588/+2159)	yfcS/yfcV	putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/15);  total (31/15)

ATCTTGTAAACGCAGCACCGCCATCGCTTTTTGACCACTGATGACGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGCCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATC  >  NC_000913/2452677‑2453162                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               aTCTTGTAAACGCAGCACCGCCATCGCTTTTTGACCACTGATGACGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCAt                                                                                                                                                                                                                                             >  2:197442/1‑251 (MQ=255)     tGTAAACGCAGCACCGCCATCGCTTTTTGACCACTGATGACGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGGTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTGCCACCGGAACATCGGCAACGTCATCg                                                                                                                                                                                                                                           <  2:163181/249‑1 (MQ=255)          aCGCAGCACCGCCATCGCTTTTTGACCACTGATGTCGGCACAATTGCGGTAGCAAATGGCTCCTTCAGTTAGCGTGAATTGCACCACCGCCTGAGGTGCTTCGGGGTTTTGCGGCACTTTGGTGAAGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTACCCCGGAACCTCGGCAACGTCATTGGCatca                                                                                                                                                                                                                                     >  1:358057/1‑250 (MQ=255)                        tCGCTTTTTGAACACTGATGACGGCACATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGAATAAACAGCAGCCCCGTTACCTTCCACCGTAACATCGGCAACGTCATCGGCATCAATCAGCGGGCGTGTc                                                                                                                                                                                                                      >  2:334514/1‑251 (MQ=255)                            tttttGACCACTGATGACGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGc                                                                                                                                                                                                                  >  1:183422/1‑251 (MQ=255)                                 gACCAGTGATGACAGCAAATTTGCGGTAGCCAATGGCTCCCTCAGTTAGCGTGGCTTGCACCACCGAATGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCatt                                                                                                                                                                                                             >  2:212159/1‑250 (MQ=255)                                        gatgaCGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCTCCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTg                                                                                                                                                                                                        <  1:319526/249‑1 (MQ=255)                                             cGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACgg                                                                                                                                                                                                 >  2:18112/1‑251 (MQ=255)                                                   aTTTGCGGTAGCCAATGGATCCTTAAGTTAGCGTGGCTTGCACCACCGACTGGGTTGATTCGGCGTTTACCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCCGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGa                                                                                                                                                                                              >  2:178788/1‑248 (MQ=255)                                                             gCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGCCTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTAACAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGt                                                                                                                                                                                 >  1:339649/1‑251 (MQ=255)                                                              ccAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTCCCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTg                                                                                                                                                                                <  2:333507/251‑1 (MQ=255)                                                                 aTGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGTGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACAGCGGCAACGTCAACGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTTCGGTGAAGTGCGCCGCCGTGGgt                                                                                                                                                                             <  2:117295/251‑1 (MQ=255)                                                                     cTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGgtagt                                                                                                                                                                          >  2:132711/1‑250 (MQ=255)                                                                           cAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGtt                                                                                                                                                                   <  1:314703/251‑1 (MQ=255)                                                                                 gCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGcgccg                                                                                                                                                             <  1:18112/251‑1 (MQ=255)                                                                                   gTGGCTTGCACCACCGACTGGGTTGCTTCGGCGCTTTCCGGAAATTTGTTGCGGTCGATATACGCCTGATTGAGATAATAATTATTAACGTCAGAAACGACGGCTTTACCACAAATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCAGCATCACTCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCgccgcc                                                                                                                                                           >  1:27533/1‑251 (MQ=255)                                                                                     ggCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCt                                                                                                                                                         >  2:133518/1‑251 (MQ=255)                                                                                               accGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACa                                                                                                                                                >  2:227722/1‑250 (MQ=255)                                                                                                  gACTGGGTTGCTTAGGCGTTTACCAGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGAGAGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTCAGCGTTGCGCCGCCCTGAAACGACaaacc                                                                                                                                            >  2:192598/1‑248 (MQ=255)                                                                                                    cTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCg                                                                                                                                          >  1:262907/1‑251 (MQ=255)                                                                                                    cTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCg                                                                                                                                          >  2:101017/1‑251 (MQ=255)                                                                                                           gCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCTCCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCTATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGGTGCGCCGCCCTGTAACGACAAGCCCGCAGAgg                                                                                                                                    <  2:193905/250‑1 (MQ=255)                                                                                                               cGGCGTTGTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTAt                                                                                                                               >  2:108446/1‑251 (MQ=255)                                                                                                                     tttCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGccc                                                                                                                          <  1:215246/250‑1 (MQ=255)                                                                                                                      ttCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCtt                                                                                                                        >  1:101419/1‑251 (MQ=255)                                                                                                                                  gttgAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCg                                                                                                            <  1:242152/251‑1 (MQ=255)                                                                                                                                  gttgAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCg                                                                                                            <  1:156243/251‑1 (MQ=255)                                                                                                                                          cGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAgg                                                                                                    >  1:6591/1‑251 (MQ=255)                                                                                                                                                 cGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCt                                                                                             >  1:70371/1‑251 (MQ=255)                                                                                                                                                                 taatTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCAGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGCACcatc                                                                             >  1:172886/1‑251 (MQ=255)                                                                                                                                                                    ttGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATg                                                                          >  1:140195/1‑251 (MQ=255)                                                                                                                                                                     tGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATgg                                                                         >  2:245890/1‑251 (MQ=255)                                                                                                                                                                      gTTAACGTCAGAAACGACTGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGTAACATCGGCAACGTCATCGGCATCAAGCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTCCGCCAGCGAACCATCATgg                                                                         <  2:350001/250‑1 (MQ=255)                                                                                                                                                                               aGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAGGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGCGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGc                                                               >  1:2709/1‑251 (MQ=255)                                                                                                                                                                               aGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGCGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGc                                                               >  1:219866/1‑251 (MQ=255)                                                                                                                                                                                 aaaCGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCa                                                             <  1:95267/251‑1 (MQ=255)                                                                                                                                                                                         gCTTTACCAAACATCTTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCt                                                     >  1:30098/1‑251 (MQ=255)                                                                                                                                                                                                  aaCATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGttt                                            >  1:197966/1‑251 (MQ=255)                                                                                                                                                                                                           gTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGt                                   <  1:108957/251‑1 (MQ=255)                                                                                                                                                                                                              taAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTgac                                >  1:11621/1‑249 (MQ=255)                                                                                                                                                                                                                   agcagcCCCGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGGATTGCCCTTCATGGTAGTTCGCACTGAGGGCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGACACTGGTGCCGATgg                           >  2:272648/1‑250 (MQ=255)                                                                                                                                                                                                                           cGTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATAAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTg                    >  2:160982/1‑250 (MQ=255)                                                                                                                                                                                                                            gTTACCTTCCACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGAt                  >  2:271319/1‑251 (MQ=255)                                                                                                                                                                                                                                    ccACCGGAACATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGt           <  1:227722/250‑1 (MQ=255)                                                                                                                                                                                                                                     cACCGGAACATCGGCAACGTCATCGGCATCACTAAGCAGACGTGACCAGCCAATATCCTCGGTCCGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGATGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGCAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTcg         >  1:21795/1‑251 (MQ=255)                                                                                                                                                                                                                                            aCATCGGCAACGTCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATc  <  2:118905/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ATCTTGTAAACGCAGCACCGCCATCGCTTTTTGACCACTGATGACGGCAAATTTGCGGTAGCCAATGGCTCCTTCAGTTAGCGTGGCTTGCACCACCGACTGGGTTGCTTCGGCGTTTTCCGGCAATTTGTTGAGGTCGATATACGCCTGATTGCGATAATAATTGTTAACGTCAGAAACGACGGCTTTACCAAACATATTGGTATAAACAGCAGCCCCGTTACCTTCCACCGGAACATCGGCAACGCCATCGGCATCAATCAGCAGGCGTGTCCCGCCCATATTCTGGGTACGGTGAAGTGCGCCGCCGTGGGTAGTAAGCGTTGCGCCGCCCTGTAACGACAAGCCCGCAGAGGTGTATTGCCCTTCATGGTAGTTCGCACTGAGGTCAACCTGCGCCAGCGAACCATCATGGCTGTAGTAGCCATCAACGCTGGTGTGTTTGTCACTGGTGCCGATGTTCAACTGATAGTGAGTCGCGTCATC  >  NC_000913/2452677‑2453162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑