Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,443,906	C→T	Q88Q (CAG→CAA)	yfcL ←	PF08891 family protein YfcL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,443,906	0	C	T	97.7%	134.4 / NA	43	Q88Q (CAG→CAA)	yfcL	PF08891 family protein YfcL
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (17/25);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCCTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACC  >  NC_000913/2443663‑2444127                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               aTGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGGGTTTGCAGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGGTAAACGTCGCATCAGGCATGCTGCTCCAGAAGCCGCAAATTAATGCTGTGAAGCTTGCTGa                                                                                                                                                                                                                          >  1:311567/1‑250 (MQ=255)   gAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAAc                                                                                                                                                                                                                       <  2:8061/251‑1 (MQ=255)        acgaACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTAAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGGAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCCTGCTGCTCCAGACGCCCCCACTTACTGCTGTTACGCTTGCTGAAACA‑GGtt                                                                                                                                                                                                                 >  1:138598/1‑251 (MQ=255)         cgaACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGttt                                                                                                                                                                                                                <  2:282501/251‑1 (MQ=255)           aaCCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTc                                                                                                                                                                                                              <  2:281140/251‑1 (MQ=255)            aCCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTcc                                                                                                                                                                                                             <  1:336095/251‑1 (MQ=255)              cGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCca                                                                                                                                                                                                           <  1:51940/251‑1 (MQ=255)                    cGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATc                                                                                                                                                                                                     <  1:304723/251‑1 (MQ=255)                         gatgCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCa                                                                                                                                                                                                <  2:40149/251‑1 (MQ=255)                            gCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCa                                                                                                                                                                                             <  1:255096/251‑1 (MQ=255)                                             gTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAAACGCGATAAGCGTCGCCTCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGGTTTCCCACATATCGGTCAACAGCGCCTGGGCACgcggg                                                                                                                                                                            >  1:378681/1‑250 (MQ=255)                                             gTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGAAATCTGATCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTTTCCGACCTACATGTGACGGTTAGGGCTTATAAAACGGGGTAAACGTCGCAACCAGCATACTGCGCCCGACGCCGCCACTTACAGCTGGGACGCCTGCTGCAACAAGGTTTTACC‑CCCACCCGTCACCAGCGCCTGGTCACggggc                                                                                                                                                                            >  2:359503/1‑251 (MQ=255)                                              ttAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCAcgcg                                                                                                                                                                              <  2:319620/248‑1 (MQ=255)                                              ttAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCAcgcg                                                                                                                                                                              >  1:319620/1‑248 (MQ=255)                                                            gTTATGGGTGCGGAAATTGTTGAAGGGAAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGGGGGAAAGCCTTATCCGAACTACGTGTGACGAGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGGCACTTACTTCTGTGACGCTTGCTGCAACA‑GGTTTTCCCACCTATTGGTCACCAGCGCCTGGGCACGCGGCGACAATTAACCAGc                                                                                                                                                              >  1:105802/1‑250 (MQ=255)                                                                 gggTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAAt                                                                                                                                                        <  1:366191/251‑1 (MQ=255)                                                                     gCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCt                                                                                                                                                    <  1:172149/251‑1 (MQ=255)                                                                      cGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCtt                                                                                                                                                   <  1:207323/251‑1 (MQ=255)                                                                         aaaTTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGGCGGGAGCGGCGTGAACGCCTTATCCGCCCTACGTGTGACGGGTAGGCCTGGTAAGACGCGATAAGACTCGCATCAGGCCTGCCCCTCCAGACGCCGCGACTTACTGCCGTGACGCTTGCTGAAACA‑GGTTTTCCCACATTACGGTCACCCGCGCCTGGTCCCGCGGCGACAATTCAACAGCAACACTGGCttttt                                                                                                                                                >  2:309839/1‑251 (MQ=255)                                                                             tgttgAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCGCGCGGCGACAATTCACCAGCACCAATGTCTTTTTCCa                                                                                                                                             <  2:28722/250‑1 (MQ=255)                                                                             tgttgAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCa                                                                                                                                             <  1:323640/250‑1 (MQ=255)                                                                                        aaaGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTTCCGGATGCGGCGTGAACGCCTTATCCGAACTACGTGGGACGTGTAGGCCTGGTAAGACGCGATAAACGTCGCATCAGGCATGCTGCCCCCGAAGCCGCAAATTACTGCCGTGGCGCTTGCTGAAACA‑GGGTTTCCCACATATCCGTCACCAGCGCCTGGGCCCGCGGCGCCCATTCACCAACACCAATGGCTTTTTTCCGActcctgcta                                                                                                                                  >  2:43516/1‑244 (MQ=255)                                                                                                              cTGCGCTCGCAGTGTTTGCTGGATGCGGCGTGAACGCCTTATCTGACCTACGTGTGACGTGTAGGCCTGATAAGAAGCGATAAGCGTCGCAGCAGGCATGATGCTCCAGACGCCGCAAATTACTGCTGTGAAGGTTGCTGAAACA‑GGGTTTCCAACATATAGGTCACCAGAGCCTGGTCACGCGGCGAAAATTCACCAGCACCAATGGCTTTTTCCAGACTCCTGCTAACGGTCGTATGCACCGCCTgcgc                                                                                                           >  2:209522/1‑248 (MQ=255)                                                                                                                gCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAg                                                                                                            <  1:226313/248‑1 (MQ=255)                                                                                                                   ctcGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTACCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGa                                                                                                        <  1:92869/249‑1 (MQ=255)                                                                                                                      gCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGGCGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTCAGCGGAGTGGt                                                                                                   >  1:208775/1‑251 (MQ=255)                                                                                                                       cAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGtc                                                                                                  >  1:26193/1‑251 (MQ=255)                                                                                                                              tGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTGTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATcacc                                                                                            <  1:225110/250‑1 (MQ=255)                                                                                                                                         gcgTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTc                                                                                <  1:190439/251‑1 (MQ=255)                                                                                                                                                cGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATg                                                                         >  1:9611/1‑251 (MQ=255)                                                                                                                                                cGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATg                                                                         >  1:94022/1‑251 (MQ=255)                                                                                                                                                         cGACCTACGTGAAACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCAATAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCAGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTc                                                                >  2:17813/1‑251 (MQ=255)                                                                                                                                                          gACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCa                                                               <  1:69975/251‑1 (MQ=255)                                                                                                                                                             cTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGt                                                            >  2:109554/1‑251 (MQ=255)                                                                                                                                                                             ggCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATAccc                                            <  2:293308/251‑1 (MQ=255)                                                                                                                                                                                                           aGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATg              <  2:261337/251‑1 (MQ=255)                                                                                                                                                                                                            ggCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCGCTGGCAAACAACTCATCATCACTGGCATgg             <  1:300961/251‑1 (MQ=255)                                                                                                                                                                                                            ggCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATgg             <  1:353249/251‑1 (MQ=255)                                                                                                                                                                                                             gCATGCTGCTCCAGCCGCCGAAACTTACTGCTGTGACGCTCGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCATGGTCACGCGGCGACACTTCAACAGCACAAATGGCCTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCCAATACCCACTGGCAAACAACTCATCATCACTgacgtggg            >  2:234306/1‑244 (MQ=255)                                                                                                                                                                                                               aTGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTACAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTc           >  1:225850/1‑250 (MQ=255)                                                                                                                                                                                                                    gctCCAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCAt     >  1:64745/1‑251 (MQ=255)                                                                                                                                                                                                                       ccAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATa    <  2:152332/249‑1 (MQ=255)                                                                                                                                                                                                                       ccAGACGCCGCAACTTACTGCTGTGACGCTTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATAcc  <  1:321808/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               ATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGCGGGGTAATCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCGATAAGCGTCGCATCAGGCATGCTGCTCCAGACGCCGCAACTTACTGCTGTGACGCCTGCTGAAACA‑GGTTTTCCCACATATCGGTCACCAGCGCCTGGTCACGCGGCGACAATTCACCAGCACCAATGGCTTTTTCCAGACTCTGGCTAACGGTCGTATGCACCGCCTGAGCGGAGTGGTCATCACCACTTTCCAGTTCTGCGATGGCTAATGTCAGGTGGCCACGCAAATACCCACTGGCAAACAACTCATCATCACTGGCATGGTCAACCATACC  >  NC_000913/2443663‑2444127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑