Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,391	G→A	E23K (GAA→AAA)	folX →	dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,391	0	G	A	100.0%	35.7 / NA	12	E23K (GAA→AAA)	folX	dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

GACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC  >  NC_000913/2421155‑2421592                                                                                                                                                                                                                                              |                                                                                                                                                                                                          gACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTaac                                                                                                                                                                                             >  2:105644/1‑251 (MQ=255)     tAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGACACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAAcc                                                                                                                                                                                         >  1:157704/1‑251 (MQ=255)      aGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTaacaac                                                                                                                                                                                          <  1:122156/249‑1 (MQ=255)              aTCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGata                                                                                                                                                                                <  2:295951/251‑1 (MQ=255)                          aGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAAtg                                                                                                                                                                    >  2:9397/1‑251 (MQ=255)                               tagCATGAGCGAATCCGTTCGCGCCCTGCCAACGGGCAGGCACTGATAAAAGCAAAACTCGGTGATGAGCGTTCGGATAGGTAACAGAAACAAATACTAGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACATCCTGCCGCTATTATTCGTATAAAGAAACTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGATCTTGTTATCAATGTGACg                                                                                                                                                               >  2:199175/3‑251 (MQ=255)                                     gAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGTTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAAACGTCATGATATTGTTAGCAATGTGACGATCCa                                                                                                                                                          <  1:97205/250‑1 (MQ=255)                                                       acTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAgcg                                                                                                                                       >  2:200324/2‑251 (MQ=255)                                                                    agaccctaaaaaaaGCACAACCCGGTGATGAGTGTTGGGATATTAAAAAAAAACGGTTTCGCGTGTTTTTTTTCATCC‑AAATAAAAAAAGGGGAAACCCGAAAATGCCACACCCTGCCTTTATTATTCGAAAAAAGACCCTTCTTTTGCGTACTTTTATCGAAATAAAGAAAAAAAAAATAAACACCCGTCAGAATTTTTTTAAAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAg                                                                                                                          <  1:117896/241‑1 (MQ=255)                                                                                                                                                                       tGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGAtg                       >  2:323609/1‑251 (MQ=255)                                                                                                                                                                                              tttattaaaaaaaaGACCCTTCGTTTGCGTACTTTTAGCGAAATTAAAAAAAAAAAAATAAACAACTATCAGTATTTTTTAAACAGAGGGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATGATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAc  <  2:179896/239‑1 (MQ=255)                                                                                                                                                                                              attattCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAc  >  1:306936/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                          GACCTAGCAATGTATCCGGCAGTCAAGAACTGGCATGAGCGGATCCGTTCGCGCCCTGCCACCGGGCAGGCACTGCTAAAAGCACAACTCGGTGATGAGCGTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAA‑TGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTTCTCTTTGCTGGAAAAATTAACTCAGGATGTGCTCGATATCGCACGTGAAC  >  NC_000913/2421155‑2421592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑