Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,373,621	G→A	intergenic (‑83/+27)	pmrD ← / ← menE	signal transduction protein PmrD/o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,621	0	G	A	100.0%	109.6 / NA	32	intergenic (‑83/+27)	pmrD/menE	signal transduction protein PmrD/o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/10);  total (22/10)

ATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACG  >  NC_000913/2373377‑2373852                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        aTAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACGTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACGCTTTATCAACGGCAATGGAATGGCAAAAATga                                                                                                                                                                                                                                   <  2:36673/251‑1 (MQ=255)        gcATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGa                                                                                                                                                                                                                            <  2:264111/251‑1 (MQ=255)            tCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGCTTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACg                                                                                                                                                                                                                        >  2:358863/1‑251 (MQ=255)                                      aCTTTCACGGCGACGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATC‑CACCAGTTTAAGTACATGTCTGTTATCTTGTTTCTTGCAGCACGATTTTTTGACCAGCCATTCCGTTGCGTTATCCTGTTTGCTAAGCGTTTGCACATCAGTTTACATCTTAACAGTTAAACTCTTTATCAACGGCAATGGAATGGCAAAACTGAGAAGTGAAACGGAGGTTTAATTATTGCTGACGTTGc                                                                                                                                                                                               >  2:61351/1‑250 (MQ=255)                                         ttCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCAccc                                                                                                                                                                                           >  1:72100/1‑251 (MQ=255)                                          tCACGGCGACGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGCGGGTTAATTATTGCTGACGTTGCAccc                                                                                                                                                                                           >  1:263991/1‑250 (MQ=255)                                                cGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCttt                                                                                                                                                                                    >  1:189455/1‑251 (MQ=255)                                                                                  cgcgCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGttt                                                                                                                                                  <  1:249218/251‑1 (MQ=255)                                                                                                    g‑agCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCggcggc                                                                                                                                   >  2:115882/1‑248 (MQ=255)                                                                                                                           tattGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGgttgtt                                                                                                          >  1:283828/3‑251 (MQ=255)                                                                                                                           tCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGgttgtt                                                                                                          >  1:283821/1‑251 (MQ=255)                                                                                                                                tttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGaaa                                                                                                      >  2:92613/1‑250 (MQ=255)                                                                                                                                tttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGaaa                                                                                                      >  1:220397/1‑250 (MQ=255)                                                                                                                                  tATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAAc                                                                                                    <  1:115882/250‑1 (MQ=255)                                                                                                                                   aTTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACgg                                                                                                  >  2:367742/1‑251 (MQ=255)                                                                                                                                                    tGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCTAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCa                                                                                  <  2:170446/250‑1 (MQ=255)                                                                                                                                                            cattccattGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCAc                                                                         >  2:286578/1‑251 (MQ=255)                                                                                                                                                                 cattGCATTATCCTGTTTGCTAAGAGTTTTCACATCAAGTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGTCGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTGTCACCCATTCACTAAGa                                                                    <  1:256216/251‑1 (MQ=255)                                                                                                                                                                    tGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTACGATc                                                                  >  2:82030/1‑250 (MQ=255)                                                                                                                                                                       aTTATCCTGTTTGTTATGTGTTTTCACATAAATTTACAAATTAACAGATAACCTCTTTATCAACGGTAATGGATAGGCAAAAAGGAGAAGTGAAACGGGGGGTTATTTATTTCTGACGTTGCACCCACTCTTTTAGCGCCTGATGAGAATTGTTAATCCCGCCTGTTTTCAGCTCCGGCGTCAGAGTTTCCCTGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCTCCCAGTCACTAAGGTCAAc                                                               <  2:249851/250‑1 (MQ=255)                                                                                                                                                                                      aaGAGTTTTCACATCAATTTCCAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATAc                                               >  1:126701/1‑251 (MQ=255)                                                                                                                                                                                                  aTCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGcc                                   >  1:267951/1‑251 (MQ=255)                                                                                                                                                                                                     aaTTTACAGATTAACAGATAAACTTTTTATCAACGGCAATGGAATGCCAAAAATGAGAATTGAACCGGAGGTTTAATTATTGCTGACTTTGCCCCCCCTCTTTTAGCGCCTGCCGTGAAATTTTAATCCCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCCCCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCAcc                                <  1:367742/251‑1 (MQ=255)                                                                                                                                                                                                      aTTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCg                               >  2:244488/1‑251 (MQ=255)                                                                                                                                                                                                                     gATACACTCTTTATCAACGGCAATGGAATGGCCAAAATGAGACGTGAAACGGCGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCCGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACt                >  2:355965/1‑251 (MQ=255)                                                                                                                                                                                                                      aTAAACTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTc               <  1:121249/251‑1 (MQ=255)                                                                                                                                                                                                                          aCTCTTTATCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTg           >  2:162403/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCa     >  2:379179/1‑250 (MQ=255)                                                                                                                                                                                                                                  tCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGGGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAACCTCCTTGTCGGCAAc   >  1:37933/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAAc   >  1:121431/1‑251 (MQ=255)                                                                                                                                                                                                                                   cAACGGCAATGGAATGGCAAAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACg  >  1:121255/1‑251 (MQ=255)                                                                                                                                                                                                                                   cAACGGCAATGGAATGGCAAAAATGAGAAGGGAAACGGAGGGTTAATTATTGCTGACGTTGCCCCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAAc   <  1:305418/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        ATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATTTTTATCGCGCCGCCAGCATCGCAG‑AGCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGGCAACG  >  NC_000913/2373377‑2373852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑