Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,365,176	G→A	P149S (CCT→TCT)	ais ←	putative lipopolysaccharide core heptose(II)‑phosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,365,176	0	G	A	100.0%	100.4 / NA	30	P149S (CCT→TCT)	ais	putative lipopolysaccharide core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/10);  total (20/10)

CGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGG  >  NC_000913/2364940‑2365415                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                cGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGt                                                                                                                                                                                                                                    >  1:217823/1‑250 (MQ=255)  gccccccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTaa                                                                                                                                                                                                                                  <  2:210811/247‑1 (MQ=255)  gCCACCCGAAAAACGTTACGTTTGAAAGGTCATCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTa                                                                                                                                                                                                                                   >  1:239894/1‑250 (MQ=255)                           aaGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAAtctc                                                                                                                                                                                                          >  2:145323/1‑250 (MQ=255)                                 ttctGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTGAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTACATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGGCCTTAATGGCACTATAAATCGCATTAcc                                                                                                                                                                                                    <  2:367781/250‑1 (MQ=255)                                                 aCAGCCATAACAGACTGTCCGATTCGAAATTTAGTGGTTAAAGAATTCCCCATCCCGATCAACTTTGCCTTTTTCCGCATGCATGACTAAACCATCCCGATCATCCGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTAAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTTAGTCCTTAATTGCACTATAACTCTCATTACCGCACTGAAGAAGTCGtt                                                                                                                                                                                   >  2:182649/1‑251 (MQ=255)                                                               cTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCCGATAAACTTTGCCTTTTTCCACCTGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGCAGTCGTTTATCTACCGTCAAt                                                                                                                                                                     >  2:164465/1‑251 (MQ=255)                                                                               ttAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGaa                                                                                                                                                      >  2:143634/1‑250 (MQ=255)                                                                                         aCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGc                                                                                                                                           >  1:24017/1‑251 (MQ=255)                                                                                         aCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGc                                                                                                                                           >  2:316772/1‑251 (MQ=255)                                                                                          cGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGcc                                                                                                                                          >  2:111579/1‑251 (MQ=255)                                                                                                 cccATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTGTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCATGTAGCCGACTGaa                                                                                                                                   <  2:179495/251‑1 (MQ=255)                                                                                                    aTCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATgg                                                                                                                                >  1:135968/1‑251 (MQ=255)                                                                                                           tAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGAc                                                                                                                         >  1:293367/1‑251 (MQ=255)                                                                                                                             acaTGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATaa                                                                                                         >  2:177271/1‑249 (MQ=255)                                                                                                                               atgcatgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGTCGACTGAATGGTCCGGACGGTATTACTGGAATaa                                                                                                         >  1:19232/1‑247 (MQ=255)                                                                                                                                                   gATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGcc                                                                                 >  2:8738/1‑250 (MQ=255)                                                                                                                                                         cAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTaaaa                                                                           <  2:60329/251‑1 (MQ=255)                                                                                                                                                                 aaTGTCGCGCCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGcc                                                                   >  1:64252/1‑251 (MQ=255)                                                                                                                                                                       gcgTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTc                                                             <  1:184863/251‑1 (MQ=255)                                                                                                                                                                            aCGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg                                                        <  2:81076/251‑1 (MQ=255)                                                                                                                                                                            aCGCTTATCTTTAGAAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACAGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACggg                                                        >  2:338461/1‑251 (MQ=255)                                                                                                                                                                                ttATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATc                                                    >  2:311058/1‑251 (MQ=255)                                                                                                                                                                                 tATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCAt                                                     <  2:102231/249‑1 (MQ=255)                                                                                                                                                                                   tCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTCTTACTGGAATAAAGATCGAAATCAGGAATATCAGCACTAAAAACGTTGCCAAATTCACGGGCATCCTg                                                 >  1:254372/1‑251 (MQ=255)                                                                                                                                                                                      ttAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTgg                                                <  1:142692/249‑1 (MQ=255)                                                                                                                                                                                                   tCAGGAAATGATTGTGGGTGAAAATAACGATATTTTTATCAGACGCTTTCCCTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGAACTGAAGAAGTCGTTTATCTACCGTCACTTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGCATAAAGATCGAAATCAGGGATATCAGCAATAAAAGCGTTGCCCAGTTCACGGGCATCATGGGTACCTTTAACCGt                                  >  2:149282/1‑250 (MQ=255)                                                                                                                                                                                                          aTGATTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTg                          <  1:143634/251‑1 (MQ=255)                                                                                                                                                                                                             aTTATGGGTGAAAATAACGATATTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGttt                       <  2:118267/251‑1 (MQ=255)                                                                                                                                                                                                                                  atTTTTATCAGACGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGTCGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTgg  >  2:269251/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                CGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGG  >  NC_000913/2364940‑2365415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑