Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	424,438	C→T	D161N (GAC→AAC)	acpH ←	acyl carrier protein phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	424,438	0	C	T	100.0%	141.8 / NA	43	D161N (GAC→AAC)	acpH	acyl carrier protein phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/28);  total (15/28)

TGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGA  >  NC_000913/424211‑424683                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gggtgCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGggc                                                                                                                                                                                                                                <  2:86619/250‑1 (MQ=255)              gaaaCCGTTTCTCAATGCCGTGCAAGGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTCGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGACAGAAGCGGGGTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGAGGGCGGCGGCTTGCCAt                                                                                                                                                                                                                   >  2:257910/4‑251 (MQ=255)              gTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCAt                                                                                                                                                                                                                   <  1:223191/251‑1 (MQ=255)                   cGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGt                                                                                                                                                                                                              <  1:1492/251‑1 (MQ=255)                           aaTGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACg                                                                                                                                                                                                      >  1:67465/1‑251 (MQ=255)                            aTGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGt                                                                                                                                                                                                     <  2:182512/251‑1 (MQ=255)                                cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGggcggcg                                                                                                                                                                                                                            >  2:274326/1‑224 (MQ=255)                                cGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGggcggcg                                                                                                                                                                                                                            <  1:274326/224‑1 (MQ=255)                                               cAAAGAAGGGCATGGGCAACTGACTGACGGGAGTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGAGGAAATCCATATc                                                                                                                                                                                  <  2:239524/251‑1 (MQ=255)                                                                   tgactgacGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACt                                                                                                                                                              >  2:285704/1‑251 (MQ=255)                                                                       tgacGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGctc                                                                                                                                                          >  1:91398/1‑251 (MQ=255)                                                                        gacGGGATGCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCAGAATGAGCGTCTAAATCTTGCCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGct                                                                                                                                                           <  1:183367/249‑1 (MQ=255)                                                                             gATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACca                                                                                                                                                    <  2:306989/251‑1 (MQ=255)                                                                                         gcctgccATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGt                                                                                                                                         <  1:93882/250‑1 (MQ=255)                                                                                               cATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGTATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGAtt                                                                                                                                  <  1:160955/251‑1 (MQ=255)                                                                                                   tCGGCTACGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGAt                                                                                                                               <  1:369952/250‑1 (MQ=255)                                                                                                    cGGCTCCGGCATGGAGGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCACCCGCGAATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTCCGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCCTTTACCACGTTCTGGATGAATTCCATATCGCGATAGCGAACCAGCCACTGCTCTGACCACAATTCATTGTGCAGATTGAtta                                                                                                                             <  2:240895/251‑3 (MQ=255)                                                                                                         aCGGTATGGATGAACTAACGTTTTATACCGTATTGCGTGACGCCTGCGCAATCATCCGCGGATAAAACTGCCAGAAGAGGGTTTCGAGGGCGTCATAATGAGCGTCTACATCGTACCAGGAGTTACGCAGGGCATCCAGAAGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGt                                                                                                                        >  1:130561/1‑251 (MQ=255)                                                                                                         aCGGTATGAATAAACTAACGTTTTATACCGTCTTGCGTGGCGCCTGCGCCAGCATCAGCGGATAAAACGGCCAGAGGCGGGTTTCGAGGGCGTCATAATTAGCGTCTAAATCGTACCAGGAGTTACTCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTACCACGTTCTGGATGAAATCCTTATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACg                                                                                                                         <  2:305544/250‑1 (MQ=255)                                                                                                          cGGTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTg                                                                                                                       >  1:248024/1‑251 (MQ=255)                                                                                                            gTATGGATGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc                                                                                                                     <  1:40227/251‑1 (MQ=255)                                                                                                                   tGAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCAATCATCCGCGGATAAAACTCCCAGAAGCGGGCTTCGAGGGCGCCAAAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGATTGCCATGCCGTTTAACACGTTCTGGATGAAATCCCTATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTAAGATTGATAAAACGTGGCGGTGAGt                                                                                                              >  1:246433/1‑251 (MQ=255)                                                                                                                    gAACTAACGTTTTATAACGTCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTc                                                                                                             <  1:377401/251‑1 (MQ=255)                                                                                                                     aaCTAACGGTGTAGAACGTCTTGCTTGACGCCTGCGCCATCAGCCGCGAATAAAACTGCCAGAAGCGGTTTTCGAGTGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACTCAGGGCATCCAGACGTGGGCGGCGGCTGGCCATGCCGGTTAACACGTTCTGTAGGAAACCCAGATCGCGAGAGCGCACCAGTCGCTGCTCTGACCACAAGTAATTTTACAGATTGATAAAACGTGGCGGTGAGTcc                                                                                                            <  1:174725/251‑1 (MQ=255)                                                                                                                           cGTTTTATAACGTCTTGCGAGACGCCTGAGCCATCATCCGCGGATAAAAATGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGAAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCggaaa                                                                                                       >  1:318239/1‑247 (MQ=255)                                                                                                                                        cTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACtt                                                                                         >  1:73687/1‑251 (MQ=255)                                                                                                                                               gACGCCTGCGCCATCATCCGCGGATAAAACTGCCCGCAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTCACCCGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGCCCACAAGTAATTGTTCAGATTGATAAAAAGTGGCGGTGAGTCCGGCAACATCGTCACCACTTGCTcgcg                                                                                  >  1:177723/1‑251 (MQ=255)                                                                                                                                               gACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTcgcg                                                                                  <  1:86817/251‑1 (MQ=255)                                                                                                                                                          caacatcCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGGAATAAGGAGCGTCTAAAACGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATAGCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAGAACGTGGCGGTGAGTCCGGCAAAATCGGGATCACTTGCTCGCGGGCATAACAGa                                                                       <  2:304251/248‑1 (MQ=255)                                                                                                                                                           atcatcCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGAc                                                                      <  2:67465/251‑1 (MQ=255)                                                                                                                                                              atcCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAACACGTGGCGGTGAGTCCGGCAAAATCGTCATCCCTTGCTCGCGGGCATAACCGACaa                                                                    >  1:100493/1‑250 (MQ=255)                                                                                                                                                                         aaaCTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCg                                                        <  1:106457/251‑1 (MQ=255)                                                                                                                                                                                   aaGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCgg                                              >  2:309521/1‑251 (MQ=255)                                                                                                                                                                                          tttCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGc                                       >  1:3796/1‑251 (MQ=255)                                                                                                                                                                                           ttCGAGGGCGTCATAATGAGCGTCTAAAGCGTACCAGGAGTTACGCGGGGCATCCTGCCGTGGGCGGCGGCTTGCCAGGCCGTTTAACACGTTCTGGATGAAATCCATAGCGCGATAGCGCCCCAGCCACTGCTCTGACCACAAGTAAGTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAAGCGTCATCACTTGCTCGCGGGCATAACAGACTAATTCCTGGAGCGGAAAGTCCGGCGACAGc                                       <  2:177723/250‑1 (MQ=255)                                                                                                                                                                                             cGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGc                                    <  1:83301/251‑1 (MQ=255)                                                                                                                                                                                                    gTCATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGt                             <  1:324333/251‑1 (MQ=255)                                                                                                                                                                                                      cATAATGAGCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGAGTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTg                            <  2:378095/250‑1 (MQ=255)                                                                                                                                                                                                              gCGTCTAAATCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAAGCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGgaaag                   <  1:16669/251‑1 (MQ=255)                                                                                                                                                                                                                    aaaTCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGt              <  1:359459/250‑1 (MQ=255)                                                                                                                                                                                                                    aaaTCGTACCAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGt              <  1:270436/250‑1 (MQ=255)                                                                                                                                                                                                                         gtcccAGGAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATccc        <  1:19430/248‑1 (MQ=255)                                                                                                                                                                                                                                gAGTTACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCAATGCCGTTTAACACGTTCTGGCTGAAATCCATATCGCGATAGCGCACAAGCCAATGCTCTGACCACAAGTACTTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATAACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGACCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAAAGATCCCACATGa  >  1:356836/1‑250 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      TGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACGCCTGCGCCATCATCCGCGGATAAAACTGCCAGAAGCGGGTTTCGAGGGCGTCATAATGAGCGTCTAAATCGTACCAGGAGTCACGCAGGGCATCCAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCGGAAAGTCCGGCGACAGCTGCGACCAGTGGCGGGAAAGAAAGTGATCCCACATGA  >  NC_000913/424211‑424683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑