Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,288	G→A	G342S (GGT→AGT)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,288	0	G	A	95.7%	129.2 / NA	46	G342S (GGT→AGT)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (24/20);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC  >  NC_000913/2355050‑2355532                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     aCAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCCCGCGGAGTGAATGGtat                                                                                                                                                                                                                                           >  2:247665/1‑250 (MQ=255)     ggcCAGTGTGTGCGCCAGGGGGGCGGGTGGATGCCGGGCGATGAAGTGAAAAAATGGACCTGTAAAAATGGCTTAGTTAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCAGTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGTTATAAggg                                                                                                                                                                                                                                      <  2:367651/250‑1 (MQ=255)     cgcCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAACAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGGAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACACACCGCCACGCGGAGTGAAGGGGATAAggg                                                                                                                                                                                                                                      >  2:180896/1‑251 (MQ=255)        cAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGgaga                                                                                                                                                                                                                                   <  1:198552/251‑1 (MQ=255)         aGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAggg                                                                                                                                                                                                                                      >  1:109879/1‑247 (MQ=255)          gTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATCTTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGCGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGGGAtt                                                                                                                                                                                                                                 >  1:123912/1‑251 (MQ=255)            ttGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGAttt                                                                                                                                                                                                                                <  1:316097/250‑1 (MQ=255)             tgtgCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGGAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATACGGGAGAttttt                                                                                                                                                                                                                              >  1:12248/1‑251 (MQ=255)                    cAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACCGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGGGAATGGGATAAGGGAGATTTTTTTTcgcc                                                                                                                                                                                                                       >  2:291160/1‑251 (MQ=255)                       ggTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTGGCGCCGCa                                                                                                                                                                                                                    <  2:28000/251‑1 (MQ=255)                       ggTGGCGTGTGGAGGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCa                                                                                                                                                                                                                    <  1:52647/251‑1 (MQ=255)                             gtgtGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTAAAAACCGCCACGCGGGGTGAATGGTATAAGGGGGATTTTTTTGCGCCGCAACCGTg                                                                                                                                                                                                              >  2:347127/1‑251 (MQ=255)                                   aTGCCGGGCGATGAACTGAAAAAAGTGACCTGTAAAAACGGCGTAGTTAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCCGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGgcagca                                                                                                                                                                                                        <  1:136384/251‑1 (MQ=255)                                     gCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                                                                                                                                      <  1:26390/251‑1 (MQ=255)                                        gggCGATGAAGTGAAAAAAGTGACCTGTAAAAAGGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCg                                                                                                                                                                                                   <  2:377022/251‑1 (MQ=255)                                                   tGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACTGAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGGGCTACAAACCGCCACGCGGGGTGAATGGGATTAGGGAGATTTTTTTTCGCCGCAACCGGGGCAGCCGTTCGGTGTCAcccct                                                                                                                                                                                        >  2:303419/1‑248 (MQ=255)                                                    gAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtg                                                                                                                                                                                       >  1:195835/1‑251 (MQ=255)                                                                   tAAAAATGGCGTAGTGAACGAAATCTGGACCCGCCATCCCGCCGACATTCCGCTCCGTCCACGTTTCGCGGTTCTCGCCAGCGGCCGTTTCTTTAGTGGCGGACTGGTAGCGGCACGTCACGGCATTAGCCAGCCGATTCTCGGCCTTGATGTGATACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACgac                                                                                                                                                                        >  1:57688/1‑249 (MQ=255)                                                                                 tGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGCACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACCCGGAGTGCATGGTCTAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCCACGCCCGTCACAGGCAggg                                                                                                                                                          >  2:253216/1‑251 (MQ=255)                                                                                                   gCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTg                                                                                                                                        <  1:363618/251‑1 (MQ=255)                                                                                                     aaTCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCCTTGAAAACCTGtt                                                                                                                                      >  1:255509/1‑251 (MQ=255)                                                                                                             cGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcg                                                                                                                              <  1:222678/251‑1 (MQ=255)                                                                                                              gATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcgg                                                                                                                             >  1:359785/1‑251 (MQ=255)                                                                                                                  ttCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACCGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAccggttcg                                                                                                                         >  2:246436/1‑251 (MQ=255)                                                                                                                             ccACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCgg                                                                                                              <  1:288166/251‑1 (MQ=255)                                                                                                                               gcGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCGCGGCCTGGATGGGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACGTCTGATGAGACGGTACGCCCGTCACAGGCAGGGCAAACCATTGAAAGCCTGTTTGGCATCGGTTCGTTGCGTGGCGGAt                                                                                                            <  2:36759/250‑1 (MQ=255)                                                                                                                                      gcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGGGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGGGGCAGCAGTTCGGTGTAACCACTGAAGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGGTTTGATcc                                                                                                     >  2:164937/1‑251 (MQ=255)                                                                                                                                       cgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGGGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTTCGCCGCAACCGTGGCAGCAGCTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGACATCGGGTCGGTGCTGGGCGGATTTTATccc                                                                                                    >  2:202469/1‑251 (MQ=255)                                                                                                                                                     ggCAGTTTCTTTAGTGGCGGACTGGTAGCGGAAAGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGATACAAACCGCCCCGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATg                                                                                      >  1:31373/1‑251 (MQ=255)                                                                                                                                                          tttCTTTAGTGGCGTACTGGTAGCGGAACGTAACGGCATTCGAGATCCGATTCTCGGCCTTGATGTGCGACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGTGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGTATgcggc                                                                                  <  2:347204/250‑1 (MQ=255)                                                                                                                                                            tCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcggc                                                                               >  1:263196/1‑251 (MQ=255)                                                                                                                                                                        gACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGcc                                                                   <  1:229269/251‑1 (MQ=255)                                                                                                                                                                           tGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTc                                                                >  1:98103/1‑251 (MQ=255)                                                                                                                                                                               aGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGt                                                             <  1:205850/250‑1 (MQ=255)                                                                                                                                                                                         aaCGGCATTCGAGAGTCGATTCTCGGCCTTGCTGTGCTCCAAACCGCCCCGCGGAGTGAATGGTATACGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAACCCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGCTCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGGGCCGTCCGTGCTTTaacagc                                                  >  2:238707/1‑246 (MQ=255)                                                                                                                                                                                                 tCGAGAGCCGTTTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTTTAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAACCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCaa                                           <  2:332447/250‑1 (MQ=255)                                                                                                                                                                                                    agagCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAAc                                          >  1:270216/1‑248 (MQ=255)                                                                                                                                                                                                                  gCCTTGATGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                         <  2:31373/251‑1 (MQ=255)                                                                                                                                                                                                                        aTGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggaggg                   >  2:1786/1‑250 (MQ=255)                                                                                                                                                                                                                        aTGTGCTACAAACCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGc                   <  2:59188/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaCCGCCACGCGGAGTGAATGGAATAAGGGAGCTTTTTTTGCGCCGCAACCGCGACAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCAGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGATGGGCGGGTTTGATCCCATCGCCCAGGGATGCGGCGGCGGGGTTTGGGCCGTACGTGCTTTACATGCCGCGCACCAGATTGCCCAACCCGCCGGAGGCCAACaatg           >  2:377918/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaCCGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCCGTGCTTTACATGCCGCTCAACAGATTGCCCCACGCGCCGGAGGCCAACaatga          >  2:32275/1‑250 (MQ=255)                                                                                                                                                                                                                                    ccGCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaatg       <  2:206955/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCCACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGac     >  1:350612/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccACGCGGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGGAGCAGTGCGGTGGAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAGCCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGGTCCCATCGCCCAGGGATGCGGCGGCGGTGTGTGTGCTGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGaca    <  2:218291/251‑1 (MQ=255)                                                                                                                                                                                                                                           gcgGAGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATCAATGACAcc  >  2:216246/1‑249 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     ACAGCGCCAGTTTGTGCGCCAGGGTGGCGTGTGGATGCCGGGCGATGAAGTGAAAAAAGTGACCTGTAAAAATGGCGTAGTGAACGAAATCTGGACCCGCAATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACC  >  NC_000913/2355050‑2355532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑