Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,340,384	C→T	intergenic (+95/+33)	ubiG → / ← yfaL	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase/putative autotransporter adhesin YfaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,340,384	0	C	T	100.0%	120.4 / NA	35	intergenic (+95/+33)	ubiG/yfaL	bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase/putative autotransporter adhesin YfaL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/22);  total (13/22)

AGAATTGCTGGGCTGGGTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCA  >  NC_000913/2340151‑2340628                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     aGAATTGCTGGGCTGGGTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTg                                                                                                                                                                                                                                     <  1:141164/251‑1 (MQ=255)   aaTTGCTGGGCTGGGTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGcc                                                                                                                                                                                                                                   <  2:146773/251‑1 (MQ=255)            gctgggTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGAc                                                                                                                                                                                                                           >  2:306885/1‑250 (MQ=255)              tgggTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAGGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGt                                                                                                                                                                                                                        >  2:144284/1‑251 (MQ=255)                            cAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATGACCATTTCAc                                                                                                                                                                                                          <  1:64370/251‑1 (MQ=255)                                    aaGAGCGGCATATCACTGGGCTGCAGTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCg                                                                                                                                                                                                  <  2:513/251‑1 (MQ=255)                                                  aCTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGcc                                                                                                                                                                                    >  1:20234/1‑251 (MQ=255)                                                                             aaTACTTTTAAACTCGGCCCCGGCGTGGATGTGAGCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTAGCAGGCCGACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGAGCAAAATCATCACTCCCtt                                                                                                                                                           <  2:182260/249‑1 (MQ=255)                                                                             aaTACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTc                                                                                                                                                         >  1:341075/1‑251 (MQ=255)                                                                              aTACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCt                                                                                                                                                        <  2:13037/251‑1 (MQ=255)                                                                                   tttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgcc                                                                                                                                                     <  1:270375/249‑1 (MQ=255)                                                                                    ttAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCATCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTgccac                                                                                                                                                   <  1:305796/250‑1 (MQ=255)                                                                                                ccGGCGTGGATGTGAACTATATGCTGCACACGCAGAAGAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCcgc                                                                                                                                      <  2:66984/251‑1 (MQ=255)                                                                                                  ggCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCgcgc                                                                                                                                    >  1:158139/1‑251 (MQ=255)                                                                                                  ggCGTGGATGGGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGGGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCCCGCCACGCTGCCgcgc                                                                                                                                    >  2:4693/1‑251 (MQ=255)                                                                                                    cGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAg                                                                                                                                  <  1:186120/251‑1 (MQ=255)                                                                                                             gAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTc                                                                                                                          <  1:231721/250‑1 (MQ=255)                                                                                                             gAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTGGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAACCCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTc                                                                                                                          <  2:322981/250‑1 (MQ=255)                                                                                                                        tgcACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGatat                                                                                                              >  2:276201/1‑251 (MQ=255)                                                                                                                                        gTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCAc                                                                                              >  1:36102/1‑251 (MQ=255)                                                                                                                                             gTTGATGTTTGGCCGCGACAATGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTACACATGCTCCCCGTAGGCCGGATCAGGCGTTTACGTCGCCTCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCACTTTCTGCCACGCCACGCTGCCGCGCAGGGACAATCGCTGACTGATATTGCCCGTGACTcctccttct                                                                                          >  2:218008/1‑243 (MQ=255)                                                                                                                                               tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTAt                                                                                        <  1:57032/250‑1 (MQ=255)                                                                                                                                                   gttaggCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTCATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGGCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCCGCCGTCTGGGCAAAAGCATCACTCCCTTTCTGCCGCGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTGTTACTTccc                                                                                   <  2:201899/247‑3 (MQ=255)                                                                                                                                                          cgcgCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTg                                                                            <  2:150834/251‑1 (MQ=255)                                                                                                                                                                aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCAc                                                                      >  1:64839/1‑251 (MQ=255)                                                                                                                                                                    ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGc                                                                   >  1:330094/1‑250 (MQ=255)                                                                                                                                                                            gACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACt                                                          >  2:99413/1‑251 (MQ=255)                                                                                                                                                                               gCTTGCCGCGTCTTATCAGGCCTACAAACGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGAt                                                       <  1:328264/251‑1 (MQ=255)                                                                                                                                                                                                 ggCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTc                                     >  1:119114/1‑251 (MQ=255)                                                                                                                                                                                                            gCTCCCCGTAGGCCGGATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCg                          <  1:348057/251‑1 (MQ=255)                                                                                                                                                                                                                           gATAAGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTGCTGCCACGCCACGCTGCCGCGCAGGGACACGCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTGCACCCCGTTGCTTCACCGCATCGGCACGGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTg             <  1:50166/249‑1 (MQ=255)                                                                                                                                                                                                                            aTAAGGCGTTTGCGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCGGGGCAAAATCATCACTCCCTTTCTGCCACGCCTCGCTGCCGCGCAGGGACACTCGCTGACTGATATTTCCCGTGACTCCCACTTTTATTTCTCCCCGTTGCTTCACCGCATCGTCACGGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGata          <  1:38785/251‑1 (MQ=255)                                                                                                                                                                                                                              aaGGCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGataat        <  1:26689/251‑1 (MQ=255)                                                                                                                                                                                                                                ggCGTTTACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGAtaataa      <  1:98008/251‑1 (MQ=255)                                                                                                                                                                                                                                     ttACGTCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATCGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCa  <  2:119114/250‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                     AGAATTGCTGGGCTGGGTGGATCAGACCAGTTTAAAAGAGCGGCATATCACTGGGCTGCATTACAACCCGATCACTAATACTTTTAAACTCGGCCCCGGCGTGGATGTGAACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAATGCTCCCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAACCGTGCCGACTAGACAGTGATTACCATTTCACCGTCATCGACAAAAACCCTGCCGTCTGGGCAAAATCATCACTCCCTTTCTGCCACGCCACGCTGCCGCGCAGGGACACTCGCTGACTGATATTGCCCGTGACTCCCACTTTTATTTCACCCCGTTGCTTCACCGCATCGTCACTGATAGTGCTGCCATCCTCTTCAATTTCCGTCGAATGGGGATCGTGATAATAATTCA  >  NC_000913/2340151‑2340628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑