Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,041	G→A	Q392Q (CAG→CAA)	atoC →	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,041	0	G	A	97.4%	134.6 / ‑6.3	38	Q392Q (CAG→CAA)	atoC	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/22);  minor base C (0/1);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATACTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCT  >  NC_000913/2322797‑2323265                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 caatgcTGCCGCCTCTGCGCGTTCGCCGGGAAGATATTTCCCTTTTAGCTAATTGCTTTTTACAAAATTTCAGGATTGAGAATCAGCTAGATTTTTTCGTCTGCGATCCTATGGCAATGTCGCTGCTTTCCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTGCAACTGTATTGAAGGCGCCGGCGTTATTAATGTAGAGCCGAGCATTTTTTCTGAGGAGCGTCCTCCACAGATTTGTCACCCAGTc                                                                                                                                                                                                                            <  2:379201/246‑1 (MQ=255)                   gcgATCGCCGGTAAGATATTTCCCTGTTAGCTAATCACGTTTTGCAAAAATACAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGTGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTa                                                                                                                                                                                                          <  1:293430/251‑1 (MQ=255)                   gcgATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGt                                                                                                                                                                                                           >  2:22707/1‑250 (MQ=255)                    cgATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTaa                                                                                                                                                                                                         <  1:167872/251‑1 (MQ=255)                     gATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTaa                                                                                                                                                                                                         >  1:232627/1‑250 (MQ=255)                            gggAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGcc                                                                                                                                                                                                 <  2:19245/251‑1 (MQ=255)                               aaGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCt                                                                                                                                                                                              <  2:201569/251‑1 (MQ=255)                                 actatTTCCCAGTTAGCTAATCACTTTTTGCAAAAATTAAGTAGTGAGAATCAGCGCGATATTCTCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCCCAGATTCGTCAACCAGTCGGTACTGCTGGAGAGGTAAAACCAGCCCCTGt                                                                                                                                                                                            >  2:243653/3‑251 (MQ=255)                                  atatTTCCCTGTTAGCTAATCACCTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGTTCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGTCGAGGTAAAAACAGCCCCTGTc                                                                                                                                                                                           >  2:350856/1‑251 (MQ=255)                                    atTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCgg                                                                                                                                                                                         >  2:134055/1‑251 (MQ=255)                                       tCCCTGTTAGCTAATCTCTTTTTGCAAAAATTCAGTAGTTAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTg                                                                                                                                                                                       <  2:299858/250‑1 (MQ=255)                                                   aaTCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTa                                                                                                                                                                            <  1:166850/249‑1 (MQ=255)                                                      cACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAag                                                                                                                                                                        <  1:18563/250‑1 (MQ=255)                                                               cAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTa                                                                                                                                                               >  1:13021/1‑250 (MQ=255)                                                                        agtagtGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGa                                                                                                                                                     <  1:213462/251‑1 (MQ=255)                                                                              gagaATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACg                                                                                                                                               >  1:317627/1‑251 (MQ=255)                                                                                gaATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc                                                                                                                                              >  2:223309/1‑250 (MQ=255)                                                                                                       tCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac                                                                                                                        >  1:316868/1‑249 (MQ=255)                                                                                                       tCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac                                                                                                                        <  2:316868/249‑1 (MQ=255)                                                                                                                    aagggcACTGGTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCAGCATTATGGAAGTGCTGGAACAACAAGAAGGAAAcc                                                                                                         <  1:158964/246‑1 (MQ=255)                                                                                                                    aaTGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAcc                                                                                                         <  1:281017/251‑1 (MQ=255)                                                                                                                       gTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGa                                                                                                       >  2:212511/1‑250 (MQ=255)                                                                                                                       gTCACTGCTTACCGCCGGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGaa                                                                                                      <  1:35520/251‑1 (MQ=255)                                                                                                                                 aCCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGACTTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGCGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGc                                                                                            >  1:210610/1‑251 (MQ=255)                                                                                                                                   cGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCtt                                                                                          <  1:15270/251‑1 (MQ=255)                                                                                                                                        ggTCATGGCCTGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGTTTTAATg                                                                                     >  2:370915/1‑251 (MQ=255)                                                                                                                                            aTGGCCGGGAAATATTCGAGCGATCTACAACGTTATTGCCCGCGCCGTCGTGATGACTTCAGGCCCCATCCTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTCATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGCGGAAATTAAAAGCGTCGCAAAACGCATCATTATGGCAGTGCTGGAACAACAAGAAGGAAAACGAACCCGCACTGCTTTAATGCTgg                                                                                 >  2:225672/1‑251 (MQ=255)                                                                                                                                                     aaaTATTCGAGAGCTTTCAAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTc                                                                        <  2:359393/251‑1 (MQ=255)                                                                                                                                                                    ttCCAACGTTATTGAACGCGCCTTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCTCCGTGCATTGATg                                                          <  2:347924/250‑1 (MQ=255)                                                                                                                                                                    ttCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATg                                                          <  2:97256/250‑1 (MQ=255)                                                                                                                                                                           gTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGCTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACt                                                  >  1:58336/1‑251 (MQ=255)                                                                                                                                                                                     ccgcCGTCGTGATGAATTCAGGCCCGATCATGTTTTCTGAGGAGCGTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATAc                                        <  2:161515/250‑1 (MQ=255)                                                                                                                                                                                      cgcCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACg                                       <  1:345680/251‑1 (MQ=255)                                                                                                                                                                                                    aTTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCcggcg                         <  2:100965/251‑1 (MQ=255)                                                                                                                                                                                                                 cATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGAATGTATAacac             <  1:212511/250‑1 (MQ=255)                                                                                                                                                                                                                       ttcggAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGAATGTATAACACCAAAAc       <  2:13021/246‑1 (MQ=255)                                                                                                                                                                                                                        tCTGAGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGTAATACGGTATCGATCCGGCGAATGTATAACACCAAAACt      <  2:371902/250‑1 (MQ=255)                                                                                                                                                                                                                            aGGATCTTCCGCCACAGATTCGTCAACCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGAATGTATAACACCAAAACTCGCt  >  1:277076/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 TAATACTGCCGCCTCTGCGCGATCGCCGGGAAGATATTTCCCTGTTAGCTAATCACTTTTTGCAAAAATTCAGTAGTGAGAATCAGCGCGATATTATCGACATCGATCCGATGGCAATGTCACTGCTTACCGCCTGGTCATGGCCGGGAAATATTCGAGAGCTTTCCAACGTTATTGAACGCGCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCT  >  NC_000913/2322797‑2323265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑