Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,296,874	G→A	P51S (CCG→TCG)	ccmB ←	cytochrome c maturation protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,296,874	0	G	A	100.0%	171.3 / NA	50	P51S (CCG→TCG)	ccmB	cytochrome c maturation protein B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

GCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTC  >  NC_000913/2296628‑2297119                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gcGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑GAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGGCACGGAACAGTCGTTCCAGCGCC‑CGCCAGGGAGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGc                                                                                                                                                                                                                                                    >  2:362782/1‑250 (MQ=255)         gcCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGGCGTTCCCGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAAACCAGATAATGCCCGATGCCATACgcgc                                                                                                                                                                                                                                           >  1:97179/1‑251 (MQ=255)                  tGCCAGCCATAATCATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTCCCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGtt                                                                                                                                                                                                                                   <  2:255127/250‑1 (MQ=255)                      agccaTAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑GAACGGCAACAACATCAATTGTTCCAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑CGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGc                                                                                                                                                                                                                              >  1:124008/1‑251 (MQ=255)                         caTAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTcc                                                                                                                                                                                                                           <  1:139169/251‑1 (MQ=255)                                    aTTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACt                                                                                                                                                                                                                <  1:67591/251‑1 (MQ=255)                                               cATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCCCCTTCGCCAGCACCACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGaaaaa                                                                                                                                                                                                     <  2:211056/251‑1 (MQ=255)                                               cATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGCCGGGCCAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGACGGCCCGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAACAGCGCAGCAACCCAGCTAATGCCCGATGCAATACGCGCCAGCCGTTGCGGCTCCGGACCGATACTGTGCGGaaaa                                                                                                                                                                                                      >  1:242780/1‑250 (MQ=255)                                                aTTGATACCAGTGGCGAATGGATAAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCCCCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCCAGGATGAAAGCAGCGCAGCAACCCATATAATGACCGATGCAATACGCGCCAGCAGTTGCGGTTCCGGACCGATACTGAGCGGAAAAAg                                                                                                                                                                                                    <  1:194212/251‑1 (MQ=255)                                                  tGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAACGCCAA‑AACCTCCATAGTTCTAGACTGCCGTCCTGCAAAACGTCACGGAACAGTCGGTCCAACGCCCAG‑AAGGAAGAAAGCAGAGGAGCCAACAAGATAATGGCCCATGCAAAACGCGACAGAAGTTGCGGCCCCGCACCGATACTGAGAGGGAAAAggg                                                                                                                                                                                                  >  1:226267/1‑251 (MQ=255)                                                              cGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCCCGGAACAGTCGGTCCAGCGCC‑AGCAAGGAAGAAAACAGCGCCGCAACCCCGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGGAATTACAATc                                                                                                                                                                                       >  1:126961/1‑250 (MQ=255)                                                                       gAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCGTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTACGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGgaagatcc                                                                                                                                                                             <  2:26481/251‑4 (MQ=255)                                                                           aaCGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACcaca                                                                                                                                                                          <  1:177435/250‑1 (MQ=255)                                                                                     cGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCg                                                                                                                                                               <  1:25175/251‑1 (MQ=255)                                                                                          aCCATCCAGTGAGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑ACACGGCCACAACATCCATTGGTTAAGACCTCCGTCCCGCCCAACGTCCCGGAAAAGTCGTTCCCACGCA‑CGCAAGGGTGAAAGCCGCGCAGCAACCCAGCTAATGCCCGATGCAATAAGCGCCCGCAGTTGCGGGTCCGGGCCGCTACTGTGCGGAGAAAGGGGAATTTCAATCAGGGAGAACACCAGCGGGTTGGCGAtttt                                                                                                                                                          >  2:183054/1‑250 (MQ=255)                                                                                                     cgcCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACAGCAATTGTTCAAGCCGGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGTGTTGGCGATTGCGGCGCTATGGc                                                                                                                                               <  2:16994/251‑1 (MQ=255)                                                                                                            aCCTTCGCCAGCACAACGGCGGGCAAGGG‑GAACGGCCACAACATCAATTTTTCAAGACTGCCGTCCTGCAAATCCCCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCCGATAATGCCCCATGCAATAAGCGCCAGCAGGTGCGGCTCCGGAACCAAACTGCGAGGGAAAAGGGGAATTACAATCAGGGAGCACCCCAACGGGTTGGCGATTTCGGCGCTATGGCGaaaagg                                                                                                                                        >  2:291202/1‑248 (MQ=255)                                                                                                                gcGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACTGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATTCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGTGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTac                                                                                                                                     <  1:253431/249‑1 (MQ=255)                                                                                                                     aGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCGAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAg                                                                                                                               <  2:196716/251‑1 (MQ=255)                                                                                                                       cacaACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCt                                                                                                                             <  2:264557/251‑1 (MQ=255)                                                                                                                          aaCGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCaa                                                                                                                          <  1:362782/251‑1 (MQ=255)                                                                                                                               cggGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACgg                                                                                                                     <  2:125286/251‑1 (MQ=255)                                                                                                                                        g‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCTAGCAGTTGCGGCTCCGGACCGATACTGAGCg                                                                                                                                                                                                           >  2:266175/1‑156 (MQ=255)                                                                                                                                        g‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCg                                                                                                                                                                                                           <  1:266175/156‑1 (MQ=255)                                                                                                                                              ggCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGa                                                                                                        >  1:109540/1‑250 (MQ=255)                                                                                                                                                             tGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTc                                                                                        >  1:36340/1‑251 (MQ=255)                                                                                                                                                             tGTTCAAGACGGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCTAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAAATACAATCAGGAAGAACCTCAGCGGGTTGGCGAGGTCGTCGCTATGGCTAAACGCTACACTGAGCTCAAGACTGAAAATGGGCCATAAcatctagcggctct                                                                                         <  2:25986/250‑11 (MQ=255)                                                                                                                                                              gTTCAAGACTGCCTGCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTc                                                                                        <  2:341423/250‑1 (MQ=255)                                                                                                                                                                  aaGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTg                                                                                   <  1:106465/251‑1 (MQ=255)                                                                                                                                                                  aaGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTg                                                                                   <  1:131860/251‑1 (MQ=255)                                                                                                                                                                              cTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAACTGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAAt                                                                       >  2:248855/1‑251 (MQ=255)                                                                                                                                                                                 cAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAAGGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATgcg                                                                    <  2:2307/251‑1 (MQ=255)                                                                                                                                                                                  aaaTCGGCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATgcgg                                                                   <  2:207451/251‑1 (MQ=255)                                                                                                                                                                                        tCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAAtt                                                             >  2:278105/1‑251 (MQ=255)                                                                                                                                                                                                 aGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCa                                                    <  2:154458/251‑1 (MQ=255)                                                                                                                                                                                                    cGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAACAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCCAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAACTGCGGCGAATTTTACTTTCAGc                                                  >  1:163533/1‑250 (MQ=255)                                                                                                                                                                                                     gTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCaa                                                <  1:200020/251‑1 (MQ=255)                                                                                                                                                                                                        ccAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACg                                              <  2:321/250‑1 (MQ=255)                                                                                                                                                                                                        ccAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACg                                              <  2:42198/250‑1 (MQ=255)                                                                                                                                                                                                            cgcC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTg                                           >  2:276279/1‑249 (MQ=255)                                                                                                                                                                                                                 aGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGc                                     <  2:159735/251‑1 (MQ=255)                                                                                                                                                                                                                   cAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCtg                                   >  2:90653/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaGCAGCGCAGCAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGt                             >  1:245877/1‑249 (MQ=255)                                                                                                                                                                                                                            aaGCAGCGCAGCAACCCAGATCATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAACTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGt                          >  2:320795/1‑251 (MQ=255)                                                                                                                                                                                                                              gcagcgcagcAACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCa                        <  1:20653/251‑1 (MQ=255)                                                                                                                                                                                                                                agcgcagcAACCCAGATACTGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTc                         >  1:92950/1‑248 (MQ=255)                                                                                                                                                                                                                                      gcaACCCAGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATcac                 >  1:132910/1‑250 (MQ=255)                                                                                                                                                                                                                                             aGATAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccc         >  2:216617/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTAATGCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATccccccc       <  1:9208/251‑1 (MQ=255)                                                                                                                                                                                                                                                    gCCCGATGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTc  >  2:117689/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GCGTCAGCGCCATCACTTGCCAGCCATAAACATCCATTCCCAGTAGCATTGCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGG‑TAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCC‑AGCAAGGATGAAAGCAGCGCAGCAACCCAGATAATGCCCGGTGCAATACGCGCCAGCAGTTGCGGCTCCGGACCGATACTGAGCGGAAAAAGGGTAATTACAATCAGGAAGAACCACAGCGGGTTGGCGATTTCGGCGCTATGGCGAAACGCTACACGCAGCTCAAGACGGAAAATGCGCCAGAACATCATGCGGCCCTCGTTTGCGTCAGTGAAATGCGGCGAATTTTACTTTCAGCAACGTTGAGCGGCTGGTGGGTAGTCAGAATCACAATCCCCCCCTGCTC  >  NC_000913/2296628‑2297119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑