Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,169,206	C→T	I165I (ATC→ATT)	yegS →	lipid kinase YegS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,169,206	0	C	T	100.0%	102.2 / NA	30	I165I (ATC→ATT)	yegS	lipid kinase YegS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

TGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATCATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATGAAATACTTCCGGCTCTCGTATCAACCTTAAAATCTGACGAAGATAA  >  NC_000913/2168960‑2169445                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                tGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACattattt                                                                                                                                                                                                                                             >  2:299936/1‑250 (MQ=255)  gatatccccGCGCTGGGAATTTTGCATTTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACAttattc                                                                                                                                                                                                                                             <  1:55489/244‑1 (MQ=255)        cccGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCtt                                                                                                                                                                                                                                      >  2:39022/1‑251 (MQ=255)                gggAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGt                                                                                                                                                                                                                               >  1:125877/1‑250 (MQ=255)                              tAGGCACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCa                                                                                                                                                                                                                 >  2:16232/1‑250 (MQ=255)                                ggAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAAcc                                                                                                                                                                                                              >  1:92220/1‑251 (MQ=255)                                                              gggATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCCCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACtt                                                                                                                                                                                <  1:238614/251‑1 (MQ=255)                                                                 aTTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCAGTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTAGTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCTCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTTGGCAACCGGACCGGTGTGAAATCCGCGGTTAAAACTTTct                                                                                                                                                                             <  2:231307/251‑2 (MQ=255)                                                                        aGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTGGGGACGCGTATTACCACAGAAACGCCGTAAAAATTAAAAGCCGCGCTGGGTATCGTCTCTTACATTATTCATGGCGTAATGCGTATGGATACTCTGGAAGCGGTCCGTTGGGAAATCCGCGGTGAAAACTTTCACTGGCaa                                                                                                                                                                      <  1:362722/251‑1 (MQ=255)                                                                           cACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACTGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGt                                                                                                                                                                   >  1:54801/1‑251 (MQ=255)                                                                                 aTAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGcc                                                                                                                                                             >  1:149472/1‑251 (MQ=255)                                                                                       cgcTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTgggc                                                                                                                                                       >  2:287019/1‑249 (MQ=255)                                                                                                 ggCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCattggtattg                                                                                                                                             >  1:220285/1‑251 (MQ=255)                                                                                                           ggagacgacATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACAGGCAAGGTGACGCCCTGGTCCTTGGTATTGGTAACGGGCg                                                                                                                                   >  2:46254/4‑251 (MQ=255)                                                                                                             tGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTc                                                                                                                                 >  1:3014/1‑251 (MQ=255)                                                                                                               aCGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAg                                                                                                                               <  1:270147/251‑1 (MQ=255)                                                                                                                          atagataTGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCgg                                                                                                                    <  1:16232/251‑1 (MQ=255)                                                                                                                           tagataTGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGt                                                                                                                   >  2:327893/1‑251 (MQ=255)                                                                                                                                atGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGc                                                                                                               <  2:313852/250‑1 (MQ=255)                                                                                                                                  ggCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGGCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAAt                                                                                                            <  1:39022/251‑1 (MQ=255)                                                                                                                                          tCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCg                                                                                                    >  1:103306/1‑251 (MQ=255)                                                                                                                                              caaacaaaCCTGTTTTATTAATATTGCGACAGGCGGATTTGGTACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAAGCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTTCCGTTCAGCAATTGTTTCCGatcg                                                                                                <  2:33450/251‑4 (MQ=255)                                                                                                                                              caaacaaaCCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAAcg                                                                                                >  1:233376/1‑251 (MQ=255)                                                                                                                                                   aaacCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGtt                                                                                            >  2:227777/1‑250 (MQ=255)                                                                                                                                                         gTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATGAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGGAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAGTTGTGTCCGAACGCTttaatta                                                                                       <  2:246481/249‑1 (MQ=255)                                                                                                                                                                           cAGGCGGATTTGGGACGCGTATTACCACAGCAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGGCTCTTACATTATTCATGGCTTAATGCGTAAGGATCCTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAACACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTACCGGGCGTCAGGCCGGTGGCGGTCAGCCATTGAGTCCGCACGCGTTAATTAACGATGGCTTGATGCAACTg                                                                   >  1:362525/1‑251 (MQ=255)                                                                                                                                                                                                      caGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATTAAATACtt                                        <  1:252047/251‑1 (MQ=255)                                                                                                                                                                                                          aaCGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCcg                                                                                                                                                                                            <  1:321998/99‑1 (MQ=255)                                                                                                                                                                                                          aaCGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCcg                                                                                                                                                                                            >  2:321998/1‑99 (MQ=255)                                                                                                                                                                                                              ccGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATGAAATACTTCCGGctct                                >  2:282656/1‑251 (MQ=255)                                                                                                                                                                                                                        tAAAAGCCGCGCTGGGTAGCGTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATGAAAGACTTTCGGCGCGCGTATCAAc                       <  1:127794/250‑1 (MQ=255)                                                                                                                                                                                                                                            gTCTCTTACATTATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATGAAATACTTCCGGCTCTCGTATCAACCTTAAAATCTGACGAAGATaa  <  1:299936/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                TGACATACCCGCGCTGGGAATTTTGCCATTAGGAACCGCCAATGATTTTGCCACCAGTGTAGGGATTCCTGAGGCACTGGATAAGGCGCTGAAACTGGCAATTGCCGGTGACGCCATTGCGATAGATATGGCGCAGGTCAACAAACAAACCTGTTTTATTAATATGGCGACAGGCGGATTTGGGACGCGTATTACCACAGAAACGCCGGAAAAATTAAAAGCCGCGCTGGGTAGCGTCTCTTACATCATTCATGGCTTAATGCGTATGGATACTCTGCAACCGGACCGTTGTGAAATCCGCGGTGAAAACTTTCACTGGCAAGGTGACGCCCTGGTCATTGGTATTGGTAACGGGCGTCAGGCCGGTGGCGGTCAGCAATTGTGTCCGAACGCGTTAATTAACGATGGCTTGCTGCAACTGCGCATTTTTACCGGCGATGAAATACTTCCGGCTCTCGTATCAACCTTAAAATCTGACGAAGATAA  >  NC_000913/2168960‑2169445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑